A new human diploid fibroblast-like cell line has been established from lung tissue of a female fetus. This has been frozen away in large quantity and characterized for use in research and related purposes. This is the first of a planned series of human cell lines to be established, characterized, and banked in large quantity in support of the National Institute on Aging research and general cell biology.

A two-dimensional computational methodology has been developed that uses a phenomenological representation of initial perturbations to model the evolution of magnetically driven Rayleigh-Taylor instabilities in a hollow Z pinch. The perturbed drive current waveform and x-ray output obtained from the two-dimensional models differ qualitatively from the results of unperturbed ͑one-dimensional͒ models. Furthermore, the perturbed results reproduce the principle features measured in a series of capacitor bank-driven pulsed power experiments. In this paper we discuss the computational approach and the computational sensitivity to initial conditions ͑including the initial perturbations͒. Representative examples are also presented of instability evolution during implosions, and the results are compared with experimentally measured current waveforms and visible framing camera images of perturbed implosions. Standard magnetohydrodynamic modeling, which includes instability growth in two dimensions, is found to reproduce the features seen in experiments.

A cell line was derived from an osteosarcoma. Cells grew to high population density in liquid medium and formed colonies in agar medium. The cell line consisted of polygonal or fusiform cells resembling the cells of the original tumor. No collagen fibers or calcium apatite microcrystals could be demonstrated in the cultured cells by electron microscopy nor were virus particles detected. Isoenzyme studies of the cell line revealed the “B” band of glucose‐6‐phosphate dehydrogenase. Chromosome studies of the cell line revealed a range of stem line number from 58 to 65; chromosome counts ranged from 47 to 183. Some cells had marker chromosome of varied morphology and some had minute chromosomes. Chromosome breaks were found in 30.5% of the cells.

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