Cultivation of corneal endothelial cells from sheep

Abdulsalam, Najla K. Al; Barnett, Nigel L.; Harkin, Damien G.; Walshe, Jennifer

doi:10.1016/j.exer.2018.04.011

Cited by 4 publications

(2 citation statements)

References 27 publications

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“…The sheep's eye has a deep anterior chamber that allows for surgical treatments, and corneal transplants undergo rejection processes that are clinically and histologically identical to those seen in humans. The ovine corneal endothelium is also essentially amitotic in vivo (Klebe et al, 2001) and again, comparable to the human corneal endothelium (Al Abdulsalam et al, 2018).…”

Section: Eyesmentioning

confidence: 82%

From waste to wealth: Repurposing slaughterhouse waste for xenotransplantation

Khan

Khraibi

Dumée

et al. 2023

Front. Bioeng. Biotechnol.

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Slaughterhouses produce large quantities of biological waste, and most of these materials are underutilized. In many published reports, the possibility of repurposing this form of waste to create biomaterials, fertilizers, biogas, and feeds has been discussed. However, the employment of particular offal wastes in xenotransplantation has yet to be extensively uncovered. Overall, viable transplantable tissues and organs are scarce, and developing bioartificial components using such discarded materials may help increase their supply. This perspective manuscript explores the viability and sustainability of readily available and easily sourced slaughterhouse waste, such as blood vessels, eyes, kidneys, and tracheas, as starting materials in xenotransplantation derived from decellularization technologies. The manuscript also examines the innovative use of animal stem cells derived from the excreta to create a bioartificial tissue/organ platform that can be translated to humans. Institutional and governmental regulatory approaches will also be outlined to support this endeavor.

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Section: Eyesmentioning

confidence: 82%

From waste to wealth: Repurposing slaughterhouse waste for xenotransplantation

Khan

Khraibi

Dumée

et al. 2023

Front. Bioeng. Biotechnol.

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“…[2] The regenerative capacity of CECs differs between species. In humans [3] and sheep, [4] CECs are arrested in a quiescent state, lacking regenerative capacity through cell division. Iatrogenic damage after surgery, genetic diseases such as Fuchs' endothelial cell dystrophy, or infections in species with non-proliferative CECs can cause a decrease in their number, compromising the tissue function and leading to corneal opacity and impaired vision, also referred as corneal endothelial dysfunction.…”

Section: Introductionmentioning

confidence: 99%

Elucidating the Corneal Endothelial Cell Proliferation Capacity through an Interspecies Transcriptome Comparison

Català

Vivensang²,

Beek

et al. 2023

Advanced Biology

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The regenerative capacity of corneal endothelial cells (CECs) differs between species; in bigger mammals, CECs are arrested in a non‐proliferative state. Damage to these cells can compromise their function causing corneal opacity. Corneal transplantation is the current treatment for the recovery of clear eyesight, but the donor tissue demand is higher than the availability and there is a need to develop novel treatments. Interestingly, rabbit CECs retain a high proliferative profile and can repopulate the endothelium. There is a lack of fundamental knowledge to explain these differences. Gaining information on their transcriptomic variances could allow the identification of CEC proliferation drivers. In this study, human, sheep, and rabbit CECs are analyzed at the transcriptomic level. To understand the differences across each species, a pipeline for the analysis of pathways with different activities is generated. The results reveal that 52 pathways have different activity when comparing species with non‐proliferative CECs (human and sheep) to species with proliferative CECs (rabbit). The results show that Notch and TGF‐β pathways have increased activity in species with non‐proliferative CECs, which might be associated with their low proliferation. Overall, this study illustrates transcriptomic pathway‐level differences that can provide leads to develop novel therapies to regenerate the corneal endothelium.

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An improved procedure for the isolation of Ribonucleic acid from methicillin-resistant Staphylococcus aureus

Al-shaibani

Zin

Jalil

et al. 2020

Novel Research in Microbiology Journal

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Extraction and purification of ribonucleic acid (RNA) from Gram-positive methicillin resistant Staphylococcus aureus (MRSA) is problematic, because the MRSA has a rigid cell wall that contains lipoteichoic acid and peptidoglycan, thus causing difficulty when utilizing the standard methods. For this reason, the aim of the current study was to improve and modify the method of extraction of RNA from MRSA, with good integrity, purity, low cost, and with saved time of extraction. A fast and an inexpensive method involving the use of acid phenol: chloroform (5: 1 [v/v]) at low pH (4.5), with lysostaphin and Triton X-100 for effective isolation of RNA from the MRSA is developed. As a result of this study, yields of this method presented high concentration of RNA 1175.26 ng/ µl/ 3 ml of bacterial culture broth, with high RNA integration number (RIN). In similar assays such as using; the RNeasy Mini kit, GeneJET RNA purification kit, TRIzol kit and hot phenol: chloroform (1: 1 [v/v]) extraction method, they yielded low concentrations of RNA (92-700 ng/ µl); with lower purity, quantity, and also little integrity, compared to using the current acid phenol chloroform (5: 1 [v/v]) extraction method. In conclusion, this new method for extraction of RNA from MRSA can be used to save time, cost, and provide high quality of RNA.

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Cultivation of corneal endothelial cells from sheep

Cited by 4 publications

References 27 publications

From waste to wealth: Repurposing slaughterhouse waste for xenotransplantation

From waste to wealth: Repurposing slaughterhouse waste for xenotransplantation

Elucidating the Corneal Endothelial Cell Proliferation Capacity through an Interspecies Transcriptome Comparison

An improved procedure for the isolation of Ribonucleic acid from methicillin-resistant Staphylococcus aureus

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