Cultivation of hepatoma cell line HepG2 on nanoporous aluminum oxide membranes

Hoess, Andreas; Teuscher, Nico; Thormann, Annika; Aurich, H; Heilmann, Andreas

doi:10.1016/j.actbio.2006.07.007

Cited by 58 publications

(40 citation statements)

References 30 publications

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“…For example, the surface chemistry and topography of a potential material for use with cells must be biologically compatible for this substrate to induce productive cell-material interactions. In the case of AAO membranes, the unique surface properties have been investigated by a number of researchers for a number of cells such as keratinocytes and fibroblasts [17], hepatocytes [18], endothelial and vascular smooth muscle cell [19], and osteoblasts [20]. The results of these studies all confirm the importance of addressing the behaviour of a specific cell line to the scaffold environment.…”

Section: Introductionsupporting

confidence: 59%

Can Anodic Aluminium Oxide Nanomembranes Treated with Nanometre Scale Hydroxyapatite be Used as a Cell Culture Substrate

Poinern

Le²,

O’Dea³

et al. 2014

IJMSA

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Abstract:In this study we investigate the biomedical potential of composite membranes composed of anodic aluminium oxide (AAO) and nanometre scale hydroxyapatite (HAP). The nano-porous AAO membranes were produced using a temperature controlled two-step anodization technique. The AAO/HAP composite membranes were formed using the solution template wetting technique. The Cercopithecus aethiops (African green monkey) Kidney (Vero) epithelial cell line was used to demonstrate the biocompatibility of the synthesised membranes and composites. Investigating cell adhesion, morphology and proliferation over a 72 h period assessed cellular interactions and responses of the cell line to the various membranes types.

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Section: Introductionsupporting

confidence: 59%

Can Anodic Aluminium Oxide Nanomembranes Treated with Nanometre Scale Hydroxyapatite be Used as a Cell Culture Substrate

Poinern

Le²,

O’Dea³

et al. 2014

IJMSA

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“…AAO membranes have also been used as nanostructured substrates for a number of different cell types. Osteoblast, 25 neutrophil, 26 hepatoma, 27 and marrow stromal 28 cell types have all been cultured on AAO membranes with demonstrated attachment, proliferation and production of ECM proteins. Here we have used AAO as a template for culture of skin derived cells (specifically keratinocytes) and as demonstrated with other cell types, keratinocytes attached, proliferated, and were viable over a long period of time on the nanoporous alumina surfaces.…”

Section: Aao Membranes As Cell Culture Substratesmentioning

confidence: 99%

The Effect of Nano-Scale Topography on Keratinocyte Phenotype and Wound Healing Following Burn Injury

Parkinson

Rea

Stevenson

et al. 2012

Tissue Engineering Part A

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“…12,13 In particular, PAA has become increasingly important in biomedical applications over the past years because of its chemical stability as well as orthopedic biomimetic and biologicallyinspired properties. [14][15][16][17][18][19] Recently, Wang et al and Hu et al studied NIH 3T3 (fibroblasts) and PC12 (pheochromocytoma) cellular behaviors on PAA with different pore sizes, but the nanopore arrays used in their study were in a disordered arrangement and pore size distribution was not even. [20][21][22] Dalby et al have previously proved that nanoscale disorder on substrates also directly influenced cellular behaviors.…”

Section: Introductionmentioning

confidence: 99%

Understanding improved osteoblast behavior on select nanoporous anodic alumina

Yan

et al. 2014

IJN

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The aim of this study was to prepare different sized porous anodic alumina (PAA) and examine preosteoblast (MC3T3-E1) attachment and proliferation on such nanoporous surfaces. In this study, PAA with tunable pore sizes (25 nm, 50 nm, and 75 nm) were fabricated by a two-step anodizing procedure in oxalic acid. The surface morphology and elemental composition of PAA were characterized by field emission scanning electron microscopy and X-ray photoelectron spectroscopy analysis. The nanopore arrays on all of the PAA samples were highly regular. X-ray photoelectron spectroscopy analysis suggested that the chemistry of PAA and flat aluminum surfaces were similar. However, contact angles were significantly greater on all of the PAA compared to flat aluminum substrates, which consequently altered protein adsorption profiles. The attachment and proliferation of preosteoblasts were determined for up to 7 days in culture using field emission scanning electron microscopy and a Cell Counting Kit-8. Results showed that nanoporous surfaces did not enhance initial preosteoblast attachment, whereas preosteoblast proliferation dramatically increased when the PAA pore size was either 50 nm or 75 nm compared to all other samples ( P <0.05). Thus, this study showed that one can alter surface energy of aluminum by modifying surface nano-roughness alone (and not changing chemistry) through an anodization process to improve osteoblast density, and, thus, should be further studied as a bioactive interface for orthopedic applications.

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Cultivation of hepatoma cell line HepG2 on nanoporous aluminum oxide membranes

Cited by 58 publications

References 30 publications

Can Anodic Aluminium Oxide Nanomembranes Treated with Nanometre Scale Hydroxyapatite be Used as a Cell Culture Substrate

Can Anodic Aluminium Oxide Nanomembranes Treated with Nanometre Scale Hydroxyapatite be Used as a Cell Culture Substrate

The Effect of Nano-Scale Topography on Keratinocyte Phenotype and Wound Healing Following Burn Injury

Understanding improved osteoblast behavior on select nanoporous anodic alumina

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