Cultivation of unculturable soil bacteria

Pham, Van Hong Thi; Kim, Jaisoo

doi:10.1016/j.tibtech.2012.05.007

Cited by 390 publications

(265 citation statements)

References 72 publications

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“…However, as more varied cultivation conditions are tested, representatives of these previously uncultured taxa are gradually being cultured [9]. These efforts should be continued, scaled up [10], and coordinated. A promising development in this respect is the recent success of culturomics (a method using high-throughput culture to enable the description of the microbial composition [11]) in human gut microbiome studies, which demonstrated a greater culturability when diverse culturing conditions were applied [12].…”

Section: The Need To Preserve Rhizosphere Microbial Diversity In Cultmentioning

confidence: 99%

PERN: an EU–Russia initiative for rhizosphere microbial resources

Declerck

Willems

Heijden

et al. 2015

Trends in Biotechnology

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Section: The Need To Preserve Rhizosphere Microbial Diversity In Cultmentioning

confidence: 99%

PERN: an EU–Russia initiative for rhizosphere microbial resources

Declerck

Willems

Heijden

et al. 2015

Trends in Biotechnology

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“…These strategies include the use of modified media, changes of growth conditions, community culture and co-culture, use of transwell plates, simulated natural environments using diffusion chambers, high-throughput microbioreactor and laser microdissection (reviewed in Pham & Kim 2012;Stewart 2012). Despite of the achievements in this field, the majority of environmental bacteria still remain recalcitrant to culturing.…”

Section: Metagenomics As a Strategy For Discovery Of Natural Productsmentioning

confidence: 99%

Bioprospecting microbial metagenome for natural products

Nováková

Farkašovský

2013

Biologia

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Mining of natural sources for new secondary metabolites has a successful history, which is reflected by the fact that over 50% of all drugs, currently on the market, are derived from natural products. Bacteria are one of the most important sources of bioactive natural products destined for drug discovery. However, less than 1% of the microorganisms observed in different habitats have been cultivated and characterized. To explore the genomic and functional diversity of the vast majority of the microbial world, novel methods were introduced, which are based on analysis of a DNA isolated from environmental communities. Metagenomics represents a strategy offering access to the genetic information present in uncultured bacteria by screening of libraries constructed from DNA isolated from different habitats. Functional-and sequence-driven screens are the major approaches employed to mine metagenomic libraries. This review aims to highlight discoveries in this area and discusses the possible future directions of the field.

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“…An estimated 99% of all bacterial species on earth are uncultured (2). These uncultured bacteria are expected to produce secondary metabolites distinct from those of known bacteria and are promising sources of new antibiotics (3).…”

Section: Introductionmentioning

confidence: 99%