Culturable halophilic bacteria inhabiting Algerian saline ecosystems: A source of promising features and potentialities

Menasria, Taha; Monteoliva‐Sánchez, Mercedes; Benammar, Leyla; Benhadj, Mabrouka; Ayachi, Ammar; Hacène, Hocine; González-Paredes, Ana; Aguilera, Margarita

doi:10.1007/s11274-019-2705-y

Cited by 41 publications

(25 citation statements)

References 49 publications

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“…A total of 128 isolates were moderately thermophilic, while 5.11% isolates were extremely or obligate thermophilic. Salt-tolerant range revealed that all the isolated strains were halotolerants [65]. The number of strains growing decreased at increasing salt.…”

Section: S21mentioning

confidence: 97%

“…Furthermore, nuclease, cellulase, lipase, and pectinase activities ranked in the first place of the common enzymes. Maybe due to the fact that the hot spring biotopes present a significant pool for special organic molecules inducing the bacterial enzymatic production [65]. Almost all the selected strains showed at least one extracellular enzyme among the tested activities, and 65.87% of bacterial isolates showed at least five combined enzymatic activities.…”

Section: G33 24g17mentioning

confidence: 99%

“…Particular interest on industrial applications of extremophiles emerges during the past few decades, because they are able to produce novel biocatalysts functioning under extreme conditions, resulting in unique products and higher production efficiency [65]. Thermozymes offer unique characteristics, such as temperature, chemical, and pH stability, resulting to technological interest in diverse industrial applications, including food, pharmaceutical, chemical, paper, textile processing, biofuel production, pulp, and waste treatment [91].…”

Section: G33 24g17mentioning

confidence: 99%

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Diversity and enzymatic potential of thermophilic bacteria associated with terrestrial hot springs in Algeria

et al. 2020

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This study aims to determine the diversity of culturable thermophilic bacteria isolated from eight terrestrial hot springs in Northeastern of Algeria using the conventional methods, SDS-PAGE fingerprinting of whole-cell proteins and 16S rRNA gene sequencing. In addition, their hydrolytic enzyme activities were also investigated. A total of 293 strains were isolated from the hot springs' water and sediment using different culture media. Overall, five distinct bacterial groups were characterized by whole-cell protein pattern analysis. Based on the 16S rRNA gene sequencing of 100 selected strains, the isolates were assigned to the following three major phyla: Firmicutes (93%), Deinococcus-Thermus (5%), and Actinobacteria (2%), which included 27 distinct species belonging to 12 different phylotypes, Aeribacillus, Aneurinibacillus, Anoxybacillus, Bacillus, Brevibacillus, Geobacillus, Laceyella, Meiothermus, Saccharomonospora, Thermoactinomyces, Thermobifida, and Thermus. The screening for nine extracellular enzymes showed that 65.87% of the isolates presented at least five types of enzyme activities, and 6.48% of strains combined all tested enzymes (amylase, cellulase, pectinase, esculinase, protease, gelatinase, lipase, lecithinase, and nuclease). It was found that Bacillus, Anoxybacillus, Aeribacillus, and Aneurinibacillus were the genera showing the highest activities. Likewise, the study showed an abundant and diverse thermophilic community with novel taxa presenting a promising source of thermozymes with important biotechnological applications. This study showed that a combined identification method using SDS-PAGE profiles of whole-cell proteins and subsequent 16S rRNA gene sequence analysis could successfully differentiate thermophilic bacteria from Algerian hot springs.

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Section: S21mentioning

confidence: 97%

Section: G33 24g17mentioning

confidence: 99%

Section: G33 24g17mentioning

confidence: 99%

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Diversity and enzymatic potential of thermophilic bacteria associated with terrestrial hot springs in Algeria

et al. 2020

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“…Three strains (A1, A2 and A4) were found to be phylogenetically attached to members of the genus Halomonas with at least 96% similarity. These strains were isolated from different sites: Chott El Beïdha, Chott Tinsilt, Chott Kralla, Sebkha Djendli, Sebkha El Hamiet, Sebkha Medghacen and Sebkha Oumache in Algerian Saline Ecosystems (Sebkha and Chott) located in arid and semi-arid ecoclimate zones (Northeastern Algeria) (Menasria et al, 2019).…”

Section: Isolation and Phylogenetic Characterization Of The Halophilimentioning

confidence: 99%

“…Furthermore, Gupta et al (2016) found that the five bacterial isolates halophilic produce one or more haloenzymes such as amylase, protease, lipase and glutaminase. Menasria et al (2019) report that the enzymes amylase, esterase, gelatinase and nuclease activities are classified first in the group of hydrolases. While Menasria et al (2018), report that the esterase and inulinase activities have the highest activities.…”

Section: Screening For Halophilic-active Hydrolytic Enzymesmentioning

confidence: 99%

Diversity of Culturable Halophilic Archaea and Bacteria from Chott Tinsilt and El Malah Salt-Lake in Algeria

Akmoussi-Toumi¹,

Khemili²,

Kebbouche-Gana³

et al. 2019

Current Research in Bioinformatics

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At the limits of life, hyper-saline aquatic ecosystems; Chott and Sebkha are a model of choice of extreme environments, housing a halophilic microflora that had to adapt to these conditions. In Algeria, these ecosystems are poorly studied. However, our study was carried out on the waters of Chott Tinsilt and Sebkha El Malah. The study of this microflora revealed the presence of a significant morphological, physiological and metabolic diversity. The molecular study allowed us to access to a phylogenetic affiliation including an Archean Species (ATS1) and 7 bacterial species (A1, A2, A3, A4, B1, B4, B5). The results showed that these isolates were related to the genera Haloferax (for the strain ATS1) and Halomonas (strains A1, A2 and A4), Staphylococcus (strain A3), Salinivibrio (strain B1), Planococcus (strain B4) and Halobacillus (strain B5). Most isolates produced hydrolases at high salt concentrations. The Production yields obtained are very promising for applications in the biotechnology and industrial microbiology.

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Purification, structural characterization, and neuroprotective effect of 3,6‐diisobutyl‐2,5‐piperazinedione from Halomonas pacifica CARE‐V15 against okadaic acid‐induced neurotoxicity in zebrafish model

Narasimman,

Ramachandran

2024

J Biochem & Molecular Tox

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Halomonas pacifica CARE‐V15 was isolated from the southeastern coast of India to determine its genome sequence. Secondary metabolite gene clusters were identified using an anti‐SMASH server. The concentrated crude ethyl acetate extract was evaluated by GC–MS. The bioactive compound from the crude ethyl acetate extract was fractionated by gel column chromatography. HPLC was used to purify the 3,6‐diisobutyl‐2,5‐piperazinedione (DIP), and the structure was determined using FTIR and NMR spectroscopy. Purified DIP was used in an in silico molecular docking analysis. Purified DIP exhibits a stronger affinity for antioxidant genes like glutathione peroxidase (GPx), glutathione‐S‐transferase (GST), and glutathione reductase (GSR). Using in silco molecular docking analysis, the protein−ligand binding affinities of GSR (−4.70 kcal/mol), GST (−5.27 kcal/mol), and GPx (−5.37 kcal/mol) were measured. The expression of antioxidant genes were investigated by qRT‐PCR. The in vivo reactive oxygen species production, lipid peroxidation, and cell death levels were significantly (p ≤ 0.05) increased in OA‐induced group, but all these levels were significantly (p ≤ 0.05) decreased in the purified DIP pretreated group. Purified DIP from halophilic bacteria could thus be a useful treatment for neurological disorders associated with oxidative stress.

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Culturable halophilic bacteria inhabiting Algerian saline ecosystems: A source of promising features and potentialities

Cited by 41 publications

References 49 publications

Diversity and enzymatic potential of thermophilic bacteria associated with terrestrial hot springs in Algeria

Diversity and enzymatic potential of thermophilic bacteria associated with terrestrial hot springs in Algeria

Diversity of Culturable Halophilic Archaea and Bacteria from Chott Tinsilt and El Malah Salt-Lake in Algeria

Purification, structural characterization, and neuroprotective effect of 3,6‐diisobutyl‐2,5‐piperazinedione from Halomonas pacifica CARE‐V15 against okadaic acid‐induced neurotoxicity in zebrafish model

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