Culturable microfungi inhibitory to A<i>rmillaria</i> rhizomorph formation from <i>Fagus sylvatica</i> stump roots and soil

Kwaśna, Hanna; Szynkiewicz‐Wronek, Anna

doi:10.1111/jph.12689

Cited by 5 publications

(7 citation statements)

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“…Further sampling of the same forest found eight fungal communities including T. polysporum and T. viride (which had previously been isolated) alongside T. koningii (Kwaśna 2004). A total of 28 fungal communities including a range of Trichoderma species ( T. aureoviride, T. hamatum, T. harzianum, T. koningii, T. strictipile and T. viride ) were isolated from beech trees (Kwaśna and Szynkiewicz‐Wronek 2018). Although isolates of T. harzianum and T. hamatum were found in the current study from roots of plants in the United Kingdom, there was no overlap with other endophytic Trichoderma species found, suggesting potential differences in geographical location.…”

Section: Discussionmentioning

confidence: 99%

“…A series of studies looked at soil micro‐fungi which were isolated from the rhizosphere and roots of oak (Kwaśna et al . 2004) and beech (Kwaśna and Szynkiewicz‐Wronek 2018) to determine inhibition against A. ostoyae and A. gallica . From oak Trichoderma spp.…”

Section: Introductionmentioning

confidence: 99%

“…2004), and in beach Trichoderma spp. could reduce rhizomorph length of A. gallica and A. ostoyae (Kwaśna and Szynkiewicz‐Wronek 2018). Crucially, control by Trichoderma tends to be isolate‐specific (Cheruiyot et al .…”

Section: Introductionmentioning

confidence: 99%

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Identification of native endophytic Trichoderma spp. for investigation of in vitro antagonism towards Armillaria mellea using synthetic‐ and plant‐based substrates

Rees

Bashir

Drakulic

et al. 2020

J. Appl. Microbiol.

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Aims To isolate endophytic Trichoderma species and investigate the potential for biological control of the root rot pathogen Armillaria mellea. Methods and Results In all, 40 Trichoderma isolates were obtained from a range of host plants and identities were confirmed by ITS, rpb2 and tef1 sequence. When tested in dual culture assays for antagonism against A. mellea, Trichoderma isolates overgrew the A. mellea colonies within four days and by eight days 38 Trichoderma isolates significantly reduced A. mellea colony size. Armillaria mellea was unable to be recovered from five of eight co‐cultivations tested, suggesting Trichoderma had killed the A. mellea in these cases. Pre‐colonized hazel disks were used to determine what happens in a more heterogeneous situation with A. mellea and a refined set of eight Trichoderma isolates. Similar to plate‐based assays, Trichoderma quickly covered A. mellea stopping any further growth and two Trichoderma isolates were able to eradicate A. mellea. Conclusions Of the Trichoderma spp. tested, endophytic isolates of Trichoderma virens and T. hamatum offered the greatest antagonism towards A. mellea. Using pre‐colonized hazel disks was of great importance for this work to demonstrate the fungal interactions in plant material. Significance and Impact of the Study Controlling Armillaria root rot is difficult with chemical treatments, thus an environmentally benign and cost‐effective alternative is required. This study highlights the prospect of biological control as an effective, environmentally friendly alternative to chemicals.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification of native endophytic Trichoderma spp. for investigation of in vitro antagonism towards Armillaria mellea using synthetic‐ and plant‐based substrates

Rees

Bashir

Drakulic

et al. 2020

J. Appl. Microbiol.

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“…Since there are no effective plant protection products in the market to control the pathogen [10], disease assessment is crucial for pest management. In fact, an accurate diagnosis in the field will improve the efficiency of prophylactic methods [7,24], such as the prompt elimination of infected plants, root residuals removal, crop rotations, and the use of less susceptible rootstocks [19]. Traditionally, the disease damage assessment was estimated using a visual approach, relying upon direct observation in the vineyard.…”

Section: Disease Diagnosismentioning

confidence: 99%

Early Identification of Root Rot Disease by Using Hyperspectral Reflectance: The Case of Pathosystem Grapevine/Armillaria

Calamita¹,

Imran²,

Vescovo³

et al. 2021

Preprint

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The Armillaria genus represents one of the most common causes of chronic root rot disease in woody plants. The disease damage prompt assessment is crucial for pest management. However, the disease detection current methods are limited at the field scale. Therefore, an alternative approach that can enhance or supplement traditional techniques is needed. In this study, we investigated the potential of hyperspectral methods to identify the changes between fungi-infected and uninfected plants of Vitis vinifera in early detecting the Armillaria disease. The hyperspectral imaging sensor Specim-IQ was used to acquire images of leaves of the Teroldego Rotaliano grapevine cultivar. We analysed three groups of plants: healthy, asymptomatic, and diseased. Highly significant differences were found in the Near infrared (NIR) spectral region with a decreasing pattern from healthy to diseased plants attributable to internal leaf structure changes. Asymptomatic plants emerged from the other groups due to a smaller reflectance in the red-edge spectrum (around 705nm). Hypothetically associated with the presence of secondary metabolites involved in plant defence strategy. Furthermore, significant differences were observed in the wavelengths close to 550 nm in diseased plants versus asymptomatic. We used linear discriminant analysis from a machine learning context to classify the leaves based on the most significant variables (vegetation indices and single bands), with resulting overall accuracies of 85% and 84% respectively in healthy vs. diseased and healthy vs. asymptomatic. To our knowledge, this study represents the first report on the possibility of using hyperspectral data for root rot disease diagnosis on woody plants. Although further validation studies are required, it appears that the spectral reflectance technique, possibly implemented on unmanned aerial vehicles (UAV), could be a promising tool for a cost-effective, non-destructive method of Armillaria disease early diagnosis and mapping in the field, contributing to a significant step forward in precision viticulture.

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“…Unfortunately, except in some rare cases (Chapman et al 2004), biological control has proven difficult to apply and has had little success in the field (Raziq and Fox 2003;Baumgartner & Warnock 2006). Even the new antagonists described in recent studies have so far failed to demonstrate their efficiency at a large scale in the field (Szwajkowska-Michalek et al 2012;Mesanza et al 2016;Kwasna & Szynkiewicz-Wronek 2018). Tomalak (2017) recently discovered nematodes of the species Bursaphelenchus fraudulentus parasiting Armillaria.…”

Section: Maximum Removal Of Roots By Machine Ripping and Removal Of Vmentioning

confidence: 99%

Latest advances and future perspectives inArmillariaresearch

Heinzelmann

Dutech

Tsykun

et al. 2019

Canadian Journal of Plant Pathology

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The basidiomycete genus Armillaria s.l. (Armillaria s.s. and Desarmillaria) has a worldwide distribution and plays a central role in the dynamics of numerous woody ecosystems, including natural forests, tree plantations for timber production, orchards, vineyards, and gardens. Early studies have shown that all Armillaria species are capable of degrading dead woody substrates causing white rot. Moreover, most species exhibit a parasitic ability, and can be considered as facultative necrotrophs. Although over the years extensive research has been conducted on the phylogeny, biology, and ecology of different Armillaria species, numerous theoretical and applied questions remain open. Recently published studies have provided new perspectives, the most significant of which we present in this review. First, new investigations have highlighted the importance of a multilocus approach for depicting the phylogeny of the genus Armillaria. Second, the importance of clonality and sexuality for the different species is now better described, enabling a more accurate prediction of population dynamics in various environments. Third, genome sequencing has provided new insights into genome evolution and the genetic basis of pathogenicity and wood degradation ability. Fourth, several new studies have pointed out the possible influence of climate change on Armillaria distribution, biology and ecology, raising questions regarding the future evolution of Armillaria species and their effect on ecosystems. In this review, we also give a state-of-the-art overview of the control possibilities of parasitic Armillaria species. Finally, we outline some still open questions in Armillaria research, the investigation of which will strongly benefit from recent methodological advances.

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Culturable microfungi inhibitory to Armillaria rhizomorph formation from Fagus sylvatica stump roots and soil

Cited by 5 publications

References 44 publications

Identification of native endophytic Trichoderma spp. for investigation of in vitro antagonism towards Armillaria mellea using synthetic‐ and plant‐based substrates

Identification of native endophytic Trichoderma spp. for investigation of in vitro antagonism towards Armillaria mellea using synthetic‐ and plant‐based substrates

Early Identification of Root Rot Disease by Using Hyperspectral Reflectance: The Case of Pathosystem Grapevine/Armillaria

Latest advances and future perspectives inArmillariaresearch

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