Cultural Characters and Serological Relationships of Some Mycoplasmas Isolated from Bovine Sources

Abstract

Cultural characteristics and serological relationships of mycoplasmas from the bovine vagina, mastitic milk, and other sources were studied. Parasitic strains could be distinguished from saprophytic strains in that they required incorporation of horse serum into the PPLO agar for growth, produced surface crystallization on PPLO agar and did not ferment carbohydrates. All strains were haemolytic for red blood cells of the guinea pig, rabbit, sheep, cow and horse. Satellite growth enhancement was seen only with the strains associated with mastitis.Rabbit antisera to each mycoplasma were tested against homologous and heterologous strains for precipitins and for growth inhibitory antibodies. Four serotypes were distinguished among the bovine isolates by agar-gel double-diffusion technique. Growth inhibitory antibodies were detected in homologous antisera against only four of the strains studied.

Mastite bovina por Mycoplasma bovis em rebanhos leiteiros

“…Sintomas semelhantes foram descritos por Jain et al (1967), Boughton (1979) e Jasper (1982. Duas vacas que ficaram sob observação por seis meses apresentaram anorexia com perda progressiva de peso.…”

NOTE: Elevation of Mycoplasma agalactiae subsp. bovis to Species Rank: Mycoplasma bovis (Hale et al.) comb. nov.

“…To involve in the studies a special reference strain for M . bouirnastitidis, however, does seem to blur the issue, which is simply a question of classification and nomenclature of a certain group of mycoplasmas, as pointed out by Jain et al (11). The serological identity of different strains within this group has never been disputed (1, 2, 14), notably not by Jain et al (ll), who, as mentioned previously, also compared strains Donetta and California 01.…”

“…They concluded that some strains, including Donetta (synonymous with rrConnecticut"), California 01, and Widanka, formed a homogenous and distinct group as determined by double immunodiffusion and growth inhibition tests. Jain et al (11) did not find the name M . agalactiae subsp.…”

“…The Subcommittee later recommended (21) that the studies be extended to include California 01 as the reference strain of M . bouirnastitidis (11). To involve in the studies a special reference strain for M .…”

“…The type strain of this proposed new taxon is Donetta (PG45) (6). Jain et al (11) studied the serological relationships of several strains associated with mastitis, isolated at different laboratories in the United States. They concluded that some strains, including Donetta (synonymous with rrConnecticut"), California 01, and Widanka, formed a homogenous and distinct group as determined by double immunodiffusion and growth inhibition tests.…”