Cultural conditions for maximum alphaamylase production by Penicillium notatum IBGE 03 using shaken flask technique of submerged fermentation

Abstract

Cultural conditions for maximum Alpha-Amylase production by Penicillium notatum IBGE 03 using shaken flask technique of submerged fermentation. AbstractEver increasing biotechnological industries require an enlarged survey of microorganisms which might be useful for industries. In the present work optimization parameters for alpha amylase production by Penicillium notatum IBGE 03 in submerged fermentation were studied. Various agricultural based by-products (sunflower waste, cotton stalk, rice husk, date syrup and molasses) were used as sources of carbon. Optimal conditions for the production of α-amylase (6.58 U/mL) by P. notatum IBGE 03 were observed when the strain was grown on culture medium M1 containing corn steep liquor as a source of nitrogen, molasses as a source of carbon after 48 h of incubation at 30° C, initial pH 5.5, inoculum size of 5x10 6 conidia in 50 mL of culture medium and agitation rate of 150 rev/min. The strain was proved thermo (up to 60° C) and pH (up to 9.0) stable so it might be a potential strain for industrial utilization.

Microbial Alpha-Amylase Production: Progress, Challenges and Perspectives

“… 49 The optimized pH for the production of alpha-amylase by Penicillium notatum IBGE 03 has been reported to be 5.5. 42 Also, Bacillus sp. MB6 produces a remarkable quantity of alpha-amylase at pH 6.…”

“…The digestive activity of alpha-amylases is measured via several colorimetric methods, including dinitrosalicylic acid method (DNS), Nelson-Somogyi, and Iodine method. 42 DNS is an alkaline reagent that attaches to the reducing sugars and then color changes can be detected by UV absorbance at 540 nm 43 (Figure 1). Amylases, xyloglucanases, pectinases, and β-mannanases are assessed by DNS method.…”

“…45 Penicillium notatum IBGE 03 is another fungus that is used for alpha-amylase production; this fungus uses molasses as its favorable carbon source. 42 Bacillus subtilis as another microorganism is used for the production of alpha-amylase in SSF and it needs glucose for optimized production of alpha-amylase is 0.02 g/g. 46 Another study on Bacillus family showed that the glucose concentration for optimum production of alpha-amylase by Bacillus amyloliquefaciens was 10.50 g/L.…”

“…52 A study on Penicillium notatum IBGE 03 used corn steep as the organic nitrogen source to optimize alpha-amylase production. 42 Soybean has been used as a source of nitrogen by Aspergillus oryzae CBS 819.72, which produced alpha-amylase on optimized condition. 36…”

Production and optimization of α-amylase from thermo-halophilic bacteria isolated from different local marine environments

“…and Brevibacillus borstelensis R1 (Singh et al 2012, Suribabu et al 2014. The production optimization studies showed that maximum enzyme production by Penicillium notatum IBGE 03 was obtained at pH 5.5 (Ahmed et al 2015). In neutral conditions (pH 6.5-7.5), the α-amylase production was reported in Bacillus sp.…”