Culturation of Hedysarum Alpinum L. Cell Culture Under in Vitro Conditions

Abstract

The purpose of the research is to develop a process for the continuous cultivation of Alpine Hedysarum cells in larger vessels (fermenters). The studies were carried out in the laboratory of biotechnology of the Federal State Budgetary Scientific Institution VILAR in 2016–2022. As a result of the experiments, a cell line was obtained from the cotyledon of a seedling of the alpine hedysarum plant, labeled as the Hedysarum alpinum (C) 2016 strain. both physiologically and morphologically. The duration of growth of the suspension culture of Hedysarum alpinum (C) 2016 corresponded to 16-20 days of cultivation, the maximum growth of the culture was 3.4 g/l of the nutrient medium, which corresponds to 24% of the growth of the cell culture in flasks on a shaker. The cell culture grown in the fermenter does not develop uniformly, as evidenced by the two peaks of biomass growth on the graph of the absolute growth rate. The factor of physiological heterogeneity of the suspension culture of Hedysarum alpinum (C) 2016 was noted, which can affect the final results on the accumulation of biomass and its biosynthetic activity. The isoflavone glycoside ononin, 7-O-(β-glucopyranosyioloxy)-4 methoxyisoflavone, was found in the cell culture of Alpine Hedysarum alpinum.