The mineral composition of Allium cepa L. leaves was measured by using the scanning electron microscope of Japanese company JEOL, model JSM600LA with EDS system. 11collection samples of FRC “All-Russian Institute of Plants Genetic Resources named after N. I. Vavilov” and 4 samples of FSBSI “Research Institute of Agriculture of the Crimea” were studied. 12 main elements (in mass., %) contained in onion leaves were evaluated. The samples with the maximum macro- and micronutrient elements accumulation in the leaves used for the sourthern subspecies breeds and hybrids selection were revealed. These samples can be used to prevent the elements deficiency in the human body. The following samples number with a high accumulation of the elements in the leaves was revealed: K – nine (from 20.0 to 3.3 max %: B12132B, trimontzium, Rouge pale, Red Wetherstfield, Blood red flat, Valensiya, Tavricheskiy, Yaltinskiy lux, Yaltinskiy rubin), P – five (from 1.8 to 2.8 mass., %:B12132B, Mestniy, Valensiya, Yaltinskiy lux, Yaltinskiy rubin), Mg – one (2.23 mass., %: Rouge pale), Ca – nine (from 5.4 to 8.3 mass., %: Mestniy, Rouge pale, Mestniy, Red Wetherstfield, Blood red flat, Valensiya, Brown Beauty, Yaltinskiy lux and Yaltinskiy rubin), Fe – two (from 0.5 to 0.8 mass., %: B12132B, Tavricheskiy), S – seven (from 2.2 to 2.5 mass., %: B12132B, Mestniy, Red Wetherstfield, Tavricheskiy, Yaltinskiy lux, Yaltinskiy rubin, Yaltinskiy model No. 3), Na – two (from 1.3 to 1.5 mass., %: B12132B, Mestniy), Cl – five (from 4.0 to 7.0 mass., %: Mestniy, B12132B, Trimontzium, Red Wetherstfield, Yaltinskiy model No. 3), Cu – one (1.9 mass., %: Yaltinskiy model No. 5), Mo – eight (from 5.2 to 7.0 mass., %: Tavricheskiy, Yaltinskiy lux, Yaltinskiy rubin, Yaltinskiy model No. 3, Mestniy, Trimontzium, Red Wetherstfield, and B12132B), Zn – seven (from 0.5 to 4.97 mass., %: Yaltinskii model No. 3, Mestniy, B12132B, Rouge pale, Blood red flat, Brown Beauty, Yaltinskiy rubin) and Si – one (0.5 mass.%,%: Yaltinskiy lux). The order of the elements accumulation variation in the onion samples was distributed as follows: Zn > Fe > Si > Na > P > Cl > Mo > Mg > S > Ca > Cu > K.

Against the background of global climate change, drought stress has become one of the environmental limiting factors that can significantly influence the growth and development of crop plants. Drought stress conditions also cause changes in plant physiological and metabolic processes. The influence of soil drought on the mineral composition of the leaves of two Actinidia species with С3-type photosynthesis, namely, Actinidia argutа (Siebold & Zucc.) Planch. ex Miq. cultivar ‘Taezhny Dar’ and Actinidia kolomikta (Maxim. & Rupr.) Maxim. cultivar ‘Narodnaya’, was studied through energy dispersive spectrometry. The investigations were carried out during 2020 to 2021 at the Department of Genofonde and Bioresources of Plants, Federal Scientific Center for Horticulture, Moscow. The present research revealed that actinidia leaves contained the following major elements: K (11.19 mass% to 13.84 mass%), Ca (7.83% to 12.08 mass%), Cl (6.20 mass% to 7.33 mass%), and Mg (2.98 mass% to 3.44 mass%). Low values were recorded for Mo (1.19 mass% to 4.49 mass%) and P (0.83 mass% to 1.25 mass%). In both species, the mineral elements K and Ca were present at high levels. A positive correlation was observed between Mg–P, K–Mn, Mn–Se, Cu-Se, P–Si, Na–Mo, and Si–Mn in the leaves of A. argutа and between Cl–Ca, Mo; P–Si, Mo; and K–Ca in the leaves of A. kolomikta. Under stress conditions, the ratios of K/Ca and K/P declined to 0.9 and 6.3, respectively, whereas those of K/Cl, K/Mg, and K/Mo increased to 3.8, 4.4, and 2.7, respectively. The present studies confirmed that actinidia leaves contained high concentrations of minerals, especially K, Ca, P, and Mg, and that the accumulation of mineral elements in actinidia plant leaves under drought conditions varied depending on the species.

Thirteen collection samples of Allium cepa L. of different ecology-geographical origin grown in the Crimea conditions are given. Morphometric characteristics of the bulbs - the form index, the diameter, the height, the weight, the thickness and the quantity of rich skins are analyzed. The greatest output of standart production (90 - 95.6%) was observed for all breeds. The biochemical values of the Allium cepa L. samples under study were examined by the traditional methods. It is marked that the dry substances, the sugars sum, the mono- and disaccharides in the majority of the samples exceed the standart (Yaltinsky rubin) on 14 - 39%, 11 - 48% and 36 - 150% correspondently. The samples with the high concentration of essential oil are singled out: Yaltinsky rubin (Crimea), Tavrichesky (Crimea), Blood red flat (Netherlands) and Brown Beauty (USA) - 4.5, 6.2, 5.6 and 4.4% correspondently. The microsculpture of Allium cepa L. leaf was studied by the method of raster electron mi-croscopy and the essential breed differences of the stomata quantity and their arrangement towards the leaf level were distinguished. The results of electron-microscopic research indicate the different level of Allium cepa L. samples adaptability. Allium cepa L. samples under study of the southern subspecies are recommended for the development of the new breeds with the advanced nutritional qualities.

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The purpose of the research is to develop a process for the continuous cultivation of Alpine Hedysarum cells in larger vessels (fermenters). The studies were carried out in the laboratory of biotechnology of the Federal State Budgetary Scientific Institution VILAR in 2016–2022. As a result of the experiments, a cell line was obtained from the cotyledon of a seedling of the alpine hedysarum plant, labeled as the Hedysarum alpinum (C) 2016 strain. both physiologically and morphologically. The duration of growth of the suspension culture of Hedysarum alpinum (C) 2016 corresponded to 16-20 days of cultivation, the maximum growth of the culture was 3.4 g/l of the nutrient medium, which corresponds to 24% of the growth of the cell culture in flasks on a shaker. The cell culture grown in the fermenter does not develop uniformly, as evidenced by the two peaks of biomass growth on the graph of the absolute growth rate. The factor of physiological heterogeneity of the suspension culture of Hedysarum alpinum (C) 2016 was noted, which can affect the final results on the accumulation of biomass and its biosynthetic activity. The isoflavone glycoside ononin, 7-O-(β-glucopyranosyioloxy)-4 methoxyisoflavone, was found in the cell culture of Alpine Hedysarum alpinum.