Culture Filtrate of Cryptococcus neoformans var. gattii (CneF) as a Novel Anti‐Inflammatory Compound in the Treatment of Experimental Septic Arthritis

Abstract

We examined the efficacy of the culture filtrate of Cryptococcus neoformans var. gattii (CneF) as a novel anti-inflammatory compound in experimental septic arthritis. Haematogenously infectious arthritis was induced in rats by a single intravenous injection of 109 CFU Staphylococcus aureus producing toxic shock syndrome toxin-1. CneF solution at two different doses (36 and 72 mg/kg, based on carbohydrate concentration) was administered intraperitoneally 2 h before bacterial inoculation in the prevention groups and simultaneously with the appearance of clinical signs in the therapeutic groups. CneF administration was continued at regular 48-h intervals for 10 injections. The results of clinical evaluation showed that CneF-treated rats were significantly protected from disease development compared with nontreated controls. This finding correlated with the results of histological and radiological assessments of the involved joints. Synovial hypertrophy, inflammatory cell infiltration, pannus formation and cartilage/bone destruction were found to be significantly decreased in the prevention and therapeutic groups compared with arthritic controls. The data suggest that CneF may have therapeutic potential in the treatment of septic arthritis.

Cryptococcus neoformansCapsular Glucuronoxylomannan Induces Expression of Fas Ligand in Macrophages

“…The mechanisms described here may be responsible for the previously described antiinflammatory effects of C. neoformans culture filtrate in experimental septic arthritis (70) and immune-mediated glomerulonephritis (71). Additional potential beneficial effects include elimination of self-reactive lymphocytes and production of antiangiogenic effects.…”

Cryptococcal capsular glucuronoxylomannan reduces ischaemia‐related neutrophil influx

“…We have demonstrated an inverse correlation between GXM titres in serum and leucocyte counts in the cerebrospinal fluids during human cryptococcal meningitis [19]. septic arthritis [21]) and in aseptic inflammation (Adriamycin-induced nephropathy [22] and immune complex glomerulonephritis [23]). In addition, cryptococcal culture filtrate (CneF ) reduced leucocyte influx and tissue damage both in models of infection (i.e.…”

The Immunomodulatory Effect of Trichophyton Rubrum Exoantigens in the Treatment of Experimental Septic Arthritis

“…This interesting point was considered by Mirshafiey et al , who suggested that the culture filtrate of Cryptococcus neoformans var. gattii (CneF) may have therapeutic potential in the treatment of septic arthritis as well as can reduce proteinuria, suppress the development of glomerular lesions, and exert lipid-lowering property in a rat model of immune complex glomerulonephritis [14, 28]. Taking all these facts into account, in the current study we investigated the immunotherapeutic ability of T. rubrum exoantigens to reduce the clinical signs and severity of septic arthritis in the artificially infected animals.…”

“…The septic arthritis was induced as formerly explained [14]. In brief, 1 ml of an S. aureus suspension containing 1 × 10 9 colony-forming units (CFU)/ml in physiological saline was intravenously injected in a lateral tail vein of each rat.…”