Culture of chondrocytes in alginate and collagen carrier gels

Susante, Job L C van; Buma, P.; Osch, Gerjo J. V. M. van; Versleyen, D.; Kraan, P.M. van der; Berg, Wim B. van den; Homminga, G.N.

doi:10.3109/17453679509002314

Cited by 172 publications

(126 citation statements)

References 37 publications

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“…Accumulating evidence has shown that chondrocytes cultured in monolayer in vitro show decreased gene expression of cartilage-specific proteins, such as type II collagen and aggrecan, and quickly dedifferentiate to a more fibroblastic phenotype (van Susante et al, 1995;Enobakhare et al, 1996). In contrast, chondrocytes cultured in alginate gels maintain their differentiated phenotype (round shape) and produce more proteoglycans and type II collagen (van Susante et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

Apoptosis in Microencapsulated Juvenile Rabbit Chondrocytes Induced by Ofloxacin: Role Played by β₁-Integrin Receptor

Sheng¹,

Peng²,

Wang³

et al. 2007

J Pharmacol Exp Ther

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Quinolone(s) (QNs) is widely used in infection therapy due to its good antimicrobial characteristics. However, QNs-induced arthropathy of immature animals has led to restrictions on the therapeutic use of these antimicrobial agents. The exact mechanism(s) of QNs-induced chondrotoxicity remain unknown. In the present study, we investigated the possible mechanism of ofloxacin (one typical QNs)-induced injuries of chondrocytes. Juvenile rabbit joint chondrocytes cultured in alginate microspheres were incubated with ofloxacin at concentrations of 0, 2, 5, 10, 20, and 40 g/ml for up to 96 h. Concentration of 10 g/ml ofloxacin induced apoptosis of chondrocyte with visible apoptotic signs, including degradation of poly(ADP-ribose) polymerase, caspase-3 activation, and DNA ladder formation. Furthermore, extracellular signal-regulated kinase 1/2 (phospho-ERK1/2) and growth factor receptor-bound protein 2 (Grb2) were significantly reduced, and similar changes were also observed in the ␤ 1 -integrin receptor as assessed by immunoblotting. However, the mRNA level of ␤ 1 -integrin obtained from reverse transcription-polymerase chain reaction remained unchanged. Results of ␤ 1 -integrin immunoprecipitation have also shown that ␤ 1 -integrin did not interact with activated intracellular signaling proteins. In addition, ofloxacin did not induce apoptosis and decrease ␤ 1 -integrin expression in chondrocytes supplemented with Mg 2ϩ , and the ofloxacin-induced apoptosis was caspase-8-dependent, inhibition of which did not affect the expression mode of phospho-ERK1/2 and ␤ 1 -integrin. Our results demonstrate that ofloxacin affects ␤ 1 -integrin receptor functions and the ERK mitogen-activated protein kinase signaling pathway, causing caspase-8-dependent apoptosis after exposure of 48 h.

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Section: Discussionmentioning

confidence: 99%

Apoptosis in Microencapsulated Juvenile Rabbit Chondrocytes Induced by Ofloxacin: Role Played by β₁-Integrin Receptor

Sheng¹,

Peng²,

Wang³

et al. 2007

J Pharmacol Exp Ther

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“…Chondrocytes maintain their functionality when embedded within biologically inert agarose gels (Benya and Shaffer, 1982), whereas encapsulation in 3D type I collagen gel accelerates dedifferentiation towards a fibroblastic phenotype (van Susante et al, 1995). The operative feature of encapsulation in 3D was not, as initially thought, the factor that exerted these effects on cell shape (Benya and Shaffer, 1982).…”

Section: Discussionmentioning

confidence: 99%

Deconstructing the third dimension – how 3D culture microenvironments alter cellular cues

Baker

Chen

2012

Journal of Cell Science

1,419

1,187

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SummaryMuch of our understanding of the biological mechanisms that underlie cellular functions, such as migration, differentiation and forcesensing has been garnered from studying cells cultured on two-dimensional (2D) glass or plastic surfaces. However, more recently the cell biology field has come to appreciate the dissimilarity between these flat surfaces and the topographically complex, threedimensional (3D) extracellular environments in which cells routinely operate in vivo. This has spurred substantial efforts towards the development of in vitro 3D biomimetic environments and has encouraged much cross-disciplinary work among biologists, material scientists and tissue engineers. As we move towards more-physiological culture systems for studying fundamental cellular processes, it is crucial to define exactly which factors are operative in 3D microenvironments. Thus, the focus of this Commentary will be on identifying and describing the fundamental features of 3D cell culture systems that influence cell structure, adhesion, mechanotransduction and signaling in response to soluble factors, which -in turn -regulate overall cellular function in ways that depart dramatically from traditional 2D culture formats. Additionally, we will describe experimental scenarios in which 3D culture is particularly relevant, highlight recent advances in materials engineering for studying cell biology, and discuss examples where studying cells in a 3D context provided insights that would not have been observed in traditional 2D systems.

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“…These have been shown to offer an attractive strategy for tissue engineering, both for the expansion of articular chondrocytes and for use as scaffold materials. 5,10,11 High-cell densities and the material properties associated with alginate impose limitations to mass transport. The use of dynamic devices, such as the RWV bioreactor, which improve diffusional gradients may enhance mass transport of nutrients to cells.…”

Section: Discussionmentioning

confidence: 99%

“…An alternative approach has involved the use of hydrogels, such as alginate, which are formed around the chondrocytes. 5,10,11 These give a uniform cell density throughout the construct 10,12 and have improved mechanical properties when compared with mesh fibrous systems, 4,8,9 thus facilitating earlier implantation.…”

mentioning

confidence: 99%

The culture of articular chondrocytes in hydrogel constructs within a bioreactor enhances cell proliferation and matrix synthesis

Akmal

Anand

et al. 2006

The Journal of Bone and Joint Surgery. British volume

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Bovine and human articular chondrocytes were seeded in 2% alginate constructs and cultured for up to 19 days in a rotating-wall-vessel (RWV) and under static conditions. Culture within the RWV enhanced DNA levels for bovine chondrocyte-seeded constructs when compared with static conditions but did not produce enhancement for human cells. There was a significant enhancement of glycosaminoglycans and hydroxyproline synthesis for both bovine and human chondrocytes. In all cases, histological analysis revealed enhanced Safranin-O staining in the peripheral regions of the constructs compared with the central region. There was an overall increase in staining intensity after culture within the RWV compared with static conditions. Type-II collagen was produced by both bovine and human chondrocytes in the peripheral and central regions of the constructs and the staining intensity was enhanced by culture within the RWV. A capsule of flattened cells containing type-I collagen developed around the constructs maintained under static conditions when seeded with either bovine or human chondrocytes, but not when cultured within the RWV bioreactor.

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Culture of chondrocytes in alginate and collagen carrier gels

Cited by 172 publications

References 37 publications

Apoptosis in Microencapsulated Juvenile Rabbit Chondrocytes Induced by Ofloxacin: Role Played by β₁-Integrin Receptor

Apoptosis in Microencapsulated Juvenile Rabbit Chondrocytes Induced by Ofloxacin: Role Played by β₁-Integrin Receptor

Deconstructing the third dimension – how 3D culture microenvironments alter cellular cues

The culture of articular chondrocytes in hydrogel constructs within a bioreactor enhances cell proliferation and matrix synthesis

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Culture of chondrocytes in alginate and collagen carrier gels

Cited by 172 publications

References 37 publications

Apoptosis in Microencapsulated Juvenile Rabbit Chondrocytes Induced by Ofloxacin: Role Played by β1-Integrin Receptor

Apoptosis in Microencapsulated Juvenile Rabbit Chondrocytes Induced by Ofloxacin: Role Played by β1-Integrin Receptor

Deconstructing the third dimension – how 3D culture microenvironments alter cellular cues

The culture of articular chondrocytes in hydrogel constructs within a bioreactor enhances cell proliferation and matrix synthesis

Contact Info

Product

Resources

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Apoptosis in Microencapsulated Juvenile Rabbit Chondrocytes Induced by Ofloxacin: Role Played by β₁-Integrin Receptor

Apoptosis in Microencapsulated Juvenile Rabbit Chondrocytes Induced by Ofloxacin: Role Played by β₁-Integrin Receptor