Culture of epithelial cells derived from the oviduct of different species

Ouhibi, Nadia; Ménézo, Yves; Benet, G.; Nicollet, Bernard

doi:10.1093/oxfordjournals.humrep.a136877

Cited by 69 publications

(38 citation statements)

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“…The success of primary fallopian tube cell culture is in accordance with other workers (7,8). The method described to obtain the primary culture was found to be the most successful of several tried in preliminary studies and in accordance with other methods reported by previous authors (9,10).…”

Section: Discussionsupporting

confidence: 88%

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Establishment of human tubal epithelial cells for coculture in an IVF program

Walker

Vlad

Kennedy³

1997

J Assist Reprod Genet

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Section: Discussionsupporting

confidence: 88%

“…There are conflicting reports in the literature on the ability successfully to passage human tubal epithelial cells over several passages. Henriksen et al (12) reported unsuccessful attempts, whereas Ouhibi et al (7) were successful.…”

Section: Discussionmentioning

confidence: 99%

Establishment of human tubal epithelial cells for coculture in an IVF program

Walker

Vlad

Kennedy³

1997

J Assist Reprod Genet

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“…The technical aspects of Vero cells maintenance have been described by Ouhibi et al [9]. Briefly, from the frozen cells, flasks (3013, Falcon, USA) were seeded with 2-3 × 10 6 cells and reached confluence within 4 days (6-8 × 10 6 cells /flask).…”

Section: Preparation Of Vero Cells and Embryo Co-culturementioning

confidence: 99%

“…The culture medium was Medium 199 (11150-059, Gibco, USA) containing 10% fetal bovine serum (FBS, 26140-079, Gibco, USA) and 0.5% antibiotics. The methods for Vero cell freezing and thawing are described by Ouhibi et al [9]. Injected and fertilized oocytes were co-cultured in DMEM supplemented with 20% hFF until transferred.…”

Section: Preparation Of Vero Cells and Embryo Co-culturementioning

confidence: 99%

A New Sperm Preparation Method for Testicular Sperm Extraction - Intracytoplasmic Sperm Injection (TESE-ICSI) Cycles: Simple, Effective and Rapid Method

Park

Song

et al. 2002

J.Mamm.Ova.Res.

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Abstract:The aim of t his study was to recover spermatozoa easily from fresh or frozen-thawed testicular sperm extraction (TESE) samples. We simply minced and washed the TESE samples in medium. Spermatozoa were recovered and washed in the bottom of a PVP droplet with an injection pipette and injected into oocytes. In all cycles 100% of spermatozoa (motile and/or immotile) could be recovered. Injected oocytes were fertilized (100% per cycle and in 41.9% of oocytes) and fertilized oocytes were cleaved (100% per cycle; 38.5% of injected oocytes; 91.8% of fertilized oocytes) a f t e r I C S I w i t h f r e s h o r f r o z e n -t h a w e d T E S E spermatozoa. The rates of progression to the embryo stage (2-8 cell) and blastocyst formation and their quality were similar in both the fresh sample group and the frozen-thawed sample group. Both of embryo transfer (73.5%) and embryo-blastocyst transfer (26.5%) were performed successfully. Thirty-four cycles of ICSI with testicular spermatozoa resulted in 23.5% pregnancy rates (fresh sample group, 22.2%; frozen-thawed sample group, 24%) with both embryo transfer (8%) and embryoblastocyst transfer (66.7%). This new sperm preparation method is very simple, easy, effective and rapid for recovering spermatozoa from TESE samples for ICSI. Key words: Fresh or frozen-thawed TESE sample, Spermatozoa recovery, ICSI, Embryo and blastocyst, PregnancyThe introduction of intracytoplasmic sperm injection (ICSI) [1] was originally considered for male factor infertility when spermatozoa could be found in the ejaculate. The possibility of achieving pregnancy with even one available spermatozoon has precipitated the search for a method to obtain spermatozoa from testicular tissue or epididymal sites, when seminal azoospermia is a constant finding. In patients with o b s t r u c t i v e a n d n o n o b st r u c t i v e a z o o s p e r m i a , fertilization and pregnancy can be accomplished by means of a combination of intracytoplasmic sperm injection with epididymal spermatozoa retrieved by microsurgical epididymal sperm aspiration or testicular sperm extraction [2][3][4][5][6][7]. Although ICSI is an effective clinical treatment strategy for male infertility patients, new types of technical difficulties have arisen, because of the extremely small number of spermatozoa handled in this procedure. The recovery of spermatozoa from extremely low quality sperm samples or TESE tissues for use in the ICSI procedure is very difficult [6][7][8].The aim of this study was to recover the spermatozoa easily from fresh or frozen-thawed TESE samples in a 3% polyvinylpyrrolidone (PVP) droplet. Materials and Methods Female patientsWomen under the age of 35 (mean: 28.7; range: 25-35 years) and having many oocytes retrieved (>10) were recruited for this study at

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“…The technical aspects of Vero cell maintenance have been described by Ouhibi et al [10]. Briefly, from the frozen cells, flasks were seeded at 2~3 × 10 6 cells and reached confluency within 4 days (6~8 × 10 6 /flask).…”

Section: Culture Of Vero Cellsmentioning

confidence: 99%

Comparison of Blastulation and Pregnancy Rates of Fertilized Human Oocytes Obtained after Conventional In Vitro Fertilization and Intracytoplasmic Sperm Injection.

et al. 2000

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Abstract:A comparison was made of good quality blastocysts obtained after either conventional in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). After IVF or ICSI, fertilized oocytes were kept in culture for a further 5 to 7 days before embryo transfer (ET) or embryo freezing. No differences were found in the number of oocytes showing two-pronuclei between conventional IVF (76.9%) and ICSI groups (85.1%). A cohort of 60 and 74 oocytes in the pronuclear stage were cultured after IVF and ICSI, respectively. The number of fertilized oocytes reaching the blastocyst stage was significantly higher (P<0.05) in the IVF group (73.3%) than in the ICSI group (56.8%). A total of 86 blastocysts were categorized into three grades depending on their morphology. The number of blastocyst embryos achieving blastocyst grade 1 (BG1) was significantly higher (P<0.05) in the IVF group than in the ICSI group, 63.3% and 13.5%, respectively. In the IVF group, 10.0% and 0% of 2PN oocytes developed to BG2 and BG3, and 2.7% and 4.1% in the ICSI group, respectively, with no significant differences between the two groups. Pregnancy rate was higher in the IVF group than in the ICSI group, 50% and 20%, respectively. It was concluded that fertilized oocytes resulting from ICSI cannnot be successfully cultured for 5-7 days for blastulation and blastocyst-ET.

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Culture of epithelial cells derived from the oviduct of different species

Cited by 69 publications

References 0 publications

Establishment of human tubal epithelial cells for coculture in an IVF program

Establishment of human tubal epithelial cells for coculture in an IVF program

A New Sperm Preparation Method for Testicular Sperm Extraction - Intracytoplasmic Sperm Injection (TESE-ICSI) Cycles: Simple, Effective and Rapid Method

Comparison of Blastulation and Pregnancy Rates of Fertilized Human Oocytes Obtained after Conventional In Vitro Fertilization and Intracytoplasmic Sperm Injection.

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