Culture of human leukemia cells

Moore, George E.; Ito, Eitaro; Ulrich, K.; Sandberg, A. A.

doi:10.1002/1097-0142(196605)19:5<713::aid-cncr2820190518>3.0.co;2-y

Cited by 135 publications

(40 citation statements)

References 23 publications

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“…These cells were originally obtained in 1965 from a 42 year-old American male with chronic myelogenous leukemia (12). The karyotype of these cells had a normal distribution of chromosomes, except for several small "marker" chromosomes that probably represented broken tetraploid metaphases (12). Normal circulating cells were obtained from adult men who were fasted for 8-12 hr.…”

Section: Methodsmentioning

confidence: 99%

“…After centrifugation in a Beckman microfuge, supernatants were discarded and the radioactivity in each pellet was determined. Cell viability was determined at the beginning and conclusion of each experiment by the Trypan Blue dye exclusion method (12). In general, 90%0 or more of the cells were viable at each determination.…”

Section: Methodsmentioning

confidence: 99%

“…Similarly, Moore et al reported that chronic growth-promoting effects of insulin could be observed with the cell line used in these studies (no. 4265) when the cells were grown in defined media or in media unsupplemented with serum (12). It is as yet unclear whether insulin produces a specific acute response in cultured or circulating lymphocytes (19,22).…”

Section: Binding Of [Mlalinsulin To Fibroblastsmentioning

confidence: 99%

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Insulin Receptors in Human Circulating Cells and Fibroblasts

Gavin¹,

Roth²,

Jen³

et al. 1972

Proc. Natl. Acad. Sci. U.S.A.

209

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Human lymphocytes obtained from fasted adult subjects and cultured human tumor lymphocytes were investigated for specific insulin receptors. By use of monoiodoinsulin, specific insulin binding sites were demonstrated in peripheral human lymphocytes, cultured human lymphocytes, and in other types of human circulating cells. Insulins and insulin derivatives that varied in their potency to stimulate glucose oxidation in the fat cell and to inhibit binding of [ 125 I]insulin to purified plasma membranes, varied in an analogous fashion in their ability to inhibit the binding of labeled insulin to human lymphocytes. Hormones that had no effect on the binding of insulin to fat cells or liver membranes also had no effect on the binding of insulin to lymphocytes. Binding was time and temperature dependent; dissociation of [ 125 I]insulin was rapid upon addition of 10 μM insulin. These findings afford a direct approach to the study of endocrine disorders in man.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Binding Of [Mlalinsulin To Fibroblastsmentioning

confidence: 99%

See 1 more Smart Citation

Insulin Receptors in Human Circulating Cells and Fibroblasts

Gavin¹,

Roth²,

Jen³

et al. 1972

Proc. Natl. Acad. Sci. U.S.A.

209

View full text Add to dashboard Cite

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“…At this time, the Roswell Park Memorial Institute (RPMI) modified the 5A medium that had been developed by McCoy,45 in terms of its calcium and magnesium concentrations. The result then was reported as the RPMI 1640 medium for use in lymphocyte cultivation 46, 47…”

Section: History Of Cell Culture Mediamentioning

confidence: 99%

Animal‐cell culture media: History, characteristics, and current issues

Yao

Asayama

2017

Reprod Medicine & Biology

394

278

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BackgroundCell culture technology has spread prolifically within a century, a variety of culture media has been designed. This review goes through the history, characteristics and current issues of animal‐cell culture media.MethodsA literature search was performed on PubMed and Google Scholar between 1880 and May 2016 using appropriate keywords.ResultsAt the dawn of cell culture technology, the major components of media were naturally derived products such as serum. The field then gradually shifted to the use of chemical‐based synthetic media because naturally derived ingredients have their disadvantages such as large batch‐to‐batch variation. Today, industrially important cells can be cultured in synthetic media. Nevertheless, the combinations and concentrations of the components in these media remain to be optimized. In addition, serum‐containing media are still in general use in the field of basic research. In the fields of assisted reproductive technologies and regenerative medicine, some of the medium components are naturally derived in nearly all instances.ConclusionsFurther improvements of culture media are desirable, which will certainly contribute to a reduction in the experimental variation, enhance productivity among biopharmaceuticals, improve treatment outcomes of assisted reproductive technologies, and facilitate implementation and popularization of regenerative medicine.

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“…They are readily available from patients with lymphoproliferative disorders as well as from healthy individuals (6)(7)(8)(9), and have an apparently indefinite life-span (10). They grow rapidly in suspension to high cell densities, retain a diploid or near-diploid karyotype in most of the cells of the culture (11)(12)(13)(14)(15)(16)(17), and maintain the differentiated ability to synthesize and secrete immunoglobulins and other immunologically active factors (18). Most, if not all, of these lines contain the Epstein-Barr virus (14,(19)(20)(21).…”

mentioning

confidence: 99%

Chemical Mutagenesis at the Phosphoribosyltransferase Locus in Cultured Human Lymphoblasts

Sato

Slesinski

Littlefield

1972

Proc. Natl. Acad. Sci. U.S.A.

114

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The presence of selectable genetic markers in long-term human lymphoblast cultures would facilitate cell hybridization experiments on the biosynthesis of immunoglobulins, as well as other studies. This work reports the induction with ethylmethane sulfonate of 6-thioguanine -resistant, phosphoribosyltransferase -deficient mutants in a lymphoblast line from a patient with infectious mononucleosis. These cells were unusually sensitive, with a Do value of 28 sg of ethylmethane sulfonate per ml; the sensitivity curve followed a biphasic pattern suggesting the presence of 3% resistant cells. Ethylmethane sulfonate increased the frequency of mutants resistant to 6-thioguanine over 100-fold, to about 2 X 10-4; nitrosoguanidine was less effective. Almost all the mutants contained considerably less than 1% of the hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8) activity of wild-type cells. The mutation did not appear to result from loss of an X chromosome.

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Culture of human leukemia cells

Cited by 135 publications

References 23 publications

Insulin Receptors in Human Circulating Cells and Fibroblasts

Insulin Receptors in Human Circulating Cells and Fibroblasts

Animal‐cell culture media: History, characteristics, and current issues

Chemical Mutagenesis at the Phosphoribosyltransferase Locus in Cultured Human Lymphoblasts

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