Culture of primary human gingival fibroblasts on biodegradable membranes

Hillmann, Georg; Steinkamp-Zucht, Angela; Geurtsen, Werner; Groß, Gerhard; Hoffmann, Andrea

doi:10.1016/s0142-9612(01)00270-8

Cited by 61 publications

(48 citation statements)

References 21 publications

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“…A cell based therapy for regeneration of tissue should utilize autologous cells, because ingrowth of immunocompetent cells of the host organism might destroy allografts or xenografts. 34 In this study, we attempted to verify the cells used for cultivation onto the biomaterials as osteoblast-like cells. Because bone chips were not treated with collagenase before cell cultivation, multipotent cells could have been present as well.…”

Section: Discussionmentioning

confidence: 99%

Comparative in vitro study of the proliferation and growth of human osteoblast‐like cells on various biomaterials

Itthichaisri

Wiedmann-Al-Ahmad

Huebner

et al. 2007

J Biomedical Materials Res

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In vitro studies about the growth behavior of osteoblasts onto biomaterials is a basic knowledge and a screening method for the development and application of scaffolds in vivo. In this in vitro study human osteoblast-like (HOB) cells were cultured on seven different biomaterials used in dental and craniomaxillofacial surgery, respectively. The tested biomaterials were synthetic biodegradable (MacroPore, Ethisorb, PDS, Beriplast P) and nonbiodegradable polymers (Palacos) as well as calcium phosphate cement (BoneSource) and titanium. The cell proliferation and cell colonization were analyzed by scanning electron microscopy and EZ4U-test. Statistical analysis were performed. HOB-like cells cultivated on Ethisorb showed the highest proliferation rate. The proliferation rate was statistically significant compared with Palacos, MacroPore, and BoneSource. Whereas, Beriplast, PDS, and titanium yielded lower proliferation rates. However, there was no statistically significant difference compared with Palacos, MacroPore, and BoneSource. SEM analysis showed no significant difference in individual cell features and cell colonization. But an infiltration and a growth of HOB-like cells throughout the porous structure of Ethisorb, which is formed by crossing fibers, is a striking different feature (macrotopography). This feature can explain the highest proliferation rate of Ethisorb. The results showed that HOB-like cells appear to be sensitive to substrate composition and topography. Moreover, the basis for further studies with such biomaterial/osteoblast constructs in vivo are provided. Further focusing points are developing techniques to fabricate three-dimensional porous biomaterial/cell constructs, studying the tissue reaction and the bone regeneration of such constructs compared with the use of autologous bone.

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Section: Discussionmentioning

confidence: 99%

Comparative in vitro study of the proliferation and growth of human osteoblast‐like cells on various biomaterials

Itthichaisri

Wiedmann-Al-Ahmad

Huebner

et al. 2007

J Biomedical Materials Res

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“…[13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29] However, it has also been shown that not all cell types show the same affi nity for a given surface. 28 The aim of the GTR procedure is the regeneration of a fully functional periodontium, including new cementum and periodontal ligament (PDL), as well as new bone.…”

Section: Cells Used For In Vitro Studiesmentioning

confidence: 99%

“…Of these, ten used human cells of either dermal (human dermal fi broblasts; HDF) or gingival (human gingival fi broblasts; HGF) origin. [17][18][19][20][21][23][24][25][26][27] Only Koop et al 22 used animal (bovine)-derived cells. Collagen sources, in contrast, were diverse, as were the modes of scaffold preparation and processing.…”

Section: Behavior Of Fi Broblasts On Collagen Membranesmentioning

confidence: 99%

Toward guided tissue and bone regeneration: morphology, attachment, proliferation, and migration of cells cultured on collagen barrier membranes. A systematic review

et al. 2008

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Collagen barrier membranes are frequently used in both guided tissue regeneration (GTR) and guided bone regeneration (GBR). Collagen used for these devices is available from different species and is often processed to alter the properties of the final product. This is necessary because unprocessed collagen is rapidly resorbed in vivo and demands for barrier membranes are different in GTR and GBR. This systematic literature review attempts to evaluate possible effects of collagen origin and mode of cross-linking on the potential of different cells to attach to, proliferate on, and migrate over barrier membranes in vitro. Seventeen original studies, selected by a systematic process, are included in this review. The results show that fibroblasts of different species and originating tissues as well as bone-forming cells are able to attach to collagen membranes irrespective of collagen origin or mode of processing. Different cell types behave differently on identical membranes. Many pieces of evidence are currently available, and we attempted to elucidate the effects of collagen origin and mode of processing on cellular behavior, but further research will be required before it will be possible to predict for certain the effect a specific procedure will have with a given product.

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“…In the present study, HGFs were chosen in order to get results closer to normal phenotype behavior in comparison with immortal cell lines (Fukuda et al 2000;Hillmann et al 2002;Locci et al 1997;Payne et al 1996;Simain-Sato et al 1999). Optical microscopy of the bottoms of wells and SEM analysis of the Inion membranes lower face confirmed the absence of cells and a positive barrier effect until 13 weeks, while the manufacturer states an 8-12 weeks in vivo barrier effect.…”

Section: Discussionmentioning

confidence: 85%

A new method using insert-based systems (IBS) to improve cell behavior study on flexible and rigid biomaterials

et al. 2016

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In vitro studies about biomaterials biological properties are essential screening tests. Yet cell cultures encounter difficulties related to cell retention on material surface or to the observation of both faces of permeable materials. The objective of the present study was to develop a reliable in vitro method to study cell behavior on rigid and flexible/permeable biomaterials elaborating two specific insert-based systems (IBS-R and IBS-F respectively). IBS-R was designed as a specific cylindrical polytetrafluoroethylene (PTFE) system to evaluate attachment, proliferation and morphology of human gingival fibroblasts (HGFs) on grade V titanium and lithium disilicate glassceramic discs characteristics of dental prostheses. The number of cells, their covering on discs and their morphology were determined from MTS assays and microscopic fluorescent images after 24, 48 and 72 h. IBS-F was developed as a two components system to study HGFs behavior on guided bone regeneration polyester membranes. The viability and the membrane barrier effect were evaluated by metabolic MTS assays and by scanning electron microscopy. IBS-R and IBS-F were shown to promote (1) easy and rapid handling; (2) cell retention on biomaterial surface; (3) accurate evaluation of the cellular proliferation, spreading and viability; (4) use of non-toxic material. Moreover IBS-F allowed the study of the cell migration through degradable membranes, with an access to both faces of the biomaterial and to the bottom of culture wells for medium changing.

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Culture of primary human gingival fibroblasts on biodegradable membranes

Cited by 61 publications

References 21 publications

Comparative in vitro study of the proliferation and growth of human osteoblast‐like cells on various biomaterials

Comparative in vitro study of the proliferation and growth of human osteoblast‐like cells on various biomaterials

Toward guided tissue and bone regeneration: morphology, attachment, proliferation, and migration of cells cultured on collagen barrier membranes. A systematic review

A new method using insert-based systems (IBS) to improve cell behavior study on flexible and rigid biomaterials

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