Culture of Saccharomyces cerevisiae on hydrolyzed waste cassava starch for production of baking-quality yeast

Ejiofor, A. O.; Chisti, Yusuf; Moo‐Young, Murray

doi:10.1016/0141-0229(95)00166-2

Cited by 53 publications

(20 citation statements)

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“…As for any fermentation [16,17], the A. latus culture performance is affected by numerous variables, including temperature, pH, carbon-to-nitrogen ratio in the feed, concentration of substrates, concentration of trace elements, ionic strength (IS), agitation intensity, and dissolved oxygen. Optimization of fermentation conditions has been used to substantially enhance yield and productivity of many bioprocesses [18,19].…”

Section: Introductionmentioning

confidence: 99%

Fermentation optimization for the production of poly(β-hydroxybutyric acid) microbial thermoplastic

Grothe

Moo‐Young

Chisti

1999

Enzyme and Microbial Technology

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195

114

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Batch culture of Alcaligenes latus, American Type Culture Collection 29713, was investigated for producing the intracellular bioplastic poly(␤-hydroxybutyric acid) (PHB). A central, composite experimental design was used to optimize the composition of the culture medium for maximizing the productivity of PHB. Investigated were the effects of temperature, the initial culture pH, the ionic strength of the medium, the concentration of trace elements, the type of nitrogen source, and the carbon-to-nitrogen ratio. The optimal temperature for growth and PHB synthesis appeared to be 33°C; however, over the 25-37°C range, the effect of temperature was negligible. An initial pH value of 6.5 gave the best results; pH values that differed even slightly from the optimum reduced the culture performance. Typical culture characteristics were: 0.075/h maximum specific growth rate, 0.38 g/l h maximum specific sucrose consumption rate, and 0.15 g/l h maximum specific PHB production rate. PHB was lost because of hydrolysis in the stationary phase, suggesting critical importance of timing the harvest. Under the best conditions, PHB constituted up to 63% of dry cell mass after 93 h of culture. The average biomass yield coefficient on sucrose was about 0.4 kg/kg. Of the four nitrogen sources-ammonium chloride, ammonium sulfate, ammonium nitrate, and urea-used, only the first two supported the culture satisfactorily. The biomass and PHB showed clear yield maxima at 1.5 g/l ammonium chloride (C:N ratio ϭ 21.5) and 1.4 g/l ammonium sulfate (C:N ratio ϭ 28.3). The yields were higher with ammonium sulfate and were relatively more sensitive to changes in its concentration. Ionic strength had a strong negative effect on PHB productivity. The highest PHB yield occurred at 4 g/l phosphate buffer concentration. Iron appeared to have the potential to enhance the proportion of PHB in the cells.

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Section: Introductionmentioning

confidence: 99%

Fermentation optimization for the production of poly(β-hydroxybutyric acid) microbial thermoplastic

Grothe

Moo‐Young

Chisti

1999

Enzyme and Microbial Technology

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195

114

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“…Therefore, the concept of using yeasts to bioconvert highcarbohydrate wastewaters has long attracted the attention of SCP researchers. High-carbohydrate wastewater for SCP processes is produced widely in many food processing industries [13], e.g., starch processing wastewater [24], waste cassava starch hydrolysate [9], deproteinized whey [25], and defatted rice polishings [5]. Recent studies have examined different vegetable processing wastewaters for yeast biomass production (Table 1), e.g., water extracts of cabbage and watermelon [26,27], pineapple cannery effluent [8,28], silage effluent [10], sugar cane bagasse hydrolysate [14], waste capsicum powder [15], and bamboo wastewater [29].…”

Section: 1mentioning

confidence: 99%

“…Since World War II, many yeast species have been used to produce SCPs from industrial wastewaters, a process that has been very important for numerous chronically malnourished people worldwide [5]. However, the conventional SCP process requires a pure yeast strain, expensive sterilization processes [6], optimized culture conditions (e.g., pH adjustment, extra nutrient supplement [N, P, Mg, Ca, Fe, Zn, Cu, Mn, and vitamins] [7], dilution rates [8]), and an air saturation of !20% dissolved oxygen (DO) by aeration [9] to achieve the maximum yeast biomass production. This results in high SCP production costs, low organic removals, and high nutrient residues that require post-treatment to control their discharge.…”

Section: Introductionmentioning

confidence: 99%

Pollutant removal-oriented yeast biomass production from high-organic-strength industrial wastewater: A review

Yang

Zheng

2014

Biomass and Bioenergy

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t r a c tMicrobial single-cell-protein (SCP) production from high-organic-strength industrial wastewaters is considered an attractive method for both wastewater purification and resource utilization. In the last two decades, pollutant removal-oriented yeast SCP production processes, i.e., yeast treatment processes, have attracted a great deal of attention from a variety of research groups worldwide. Different from conventional SCP production processes, yeast treatment processes are characterized by higher pollutant removal rates, lower production costs, highly adaptive yeast isolates from nature, no excess nutrient supplements, and are performed under non-sterile conditions. Furthermore, yeast treatment processes are similar to bacteria-dominated conventional activated sludge processes, which offer more choices for yeast SCP production and industrial wastewater treatment.This review discusses why highly adaptive yeast species isolated from nature are used in the yeast treatment process rather than commercial SCP producers. It also describes the application of yeast treatment processes for treating high-carboxyhydrate, oil-rich and high-salinity industrial wastewater, focusing primarily on high-strength biodegradable organic substances, which usually account for the major fraction of biochemical oxygen demand. Also discussed is the biodegradation of xenobiotics, such as color (including dye and pigment) and toxic substances (including phenols, chlorophenols, polycyclic aromatic hydrocarbons, etc.), present in industrial wastewater. Based on molecular information of yeast community structures and their regulation in yeast treatment systems, we also discuss how to maintain efficient yeast species in yeast biomass and how to control bacterial and mold proliferation in yeast treatment systems.ª 2014 Elsevier Ltd. All rights reserved. IntroductionYeasts are a group of unicellular fungi widely distributed in nature, most of which belong to two separate phyla: the Ascomycota and the Basidiomycota. Yeasts have played * Corresponding author. Tel.: þ86 10 58809266. E-mail address: zsk@bnu.edu.cn (S. Zheng).Available online at www.sciencedirect.com ScienceDirect ht tp://www.elsevier.com/locate/biombioe b i o m a s s a n d b i o e n e r g y 6 4 ( 2 0 1 4 ) 3 5 6 e3 6 2 http://dx

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“…Singh et al (1995) found that protein hydrolysates could increase the biomass of S. platensis and GLA deposited in its biomass. Medium composition is one of the major contributor to the productivity of microbial metabolites in their biomass such as GLA (Botha et al, 1997) and protein (Eijifor et al, 1995). The use of low-cost nutrition such as latex serum that is economically unutilized and even to be waste, together with technical grade chemicals in optimal medium was considered capable of reducing production cost of S. platensis biomass.…”

Section: Introductionmentioning

confidence: 99%

Optimization media from low-cost nutrient sources for growing Spirulina platensis and carotenoid production Optimasi media dengan sumber nutrisi murah untuk pertumbuhan dan produksi karotenoid Spirulina platensis

TRI-PANJI¹,

SUHARYANTO²

2016

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SummarySpirulina platensis is a cyanobacteriaproducing several bioactive compounds such ascarotenoids which are economically valuable. Toproduce carotenoids in S. platensis biomassefficiently, it is necessary to define an optimummedium composition consisting of mineral saltand organic complex derived from low-costnutrient sources. Spirulina platensis grown oncomplex media containing latex serum fromconcentrated latex factory, supplemented withsalt minerals might produce high yieldingcarotenoids. The objective of this research is todefine media composition for optimum growthand carotenoid production of S. platensis and toidentify carotenoid compounds from biomass ofthe algae. S. platensis was grown on mediacontaining latex serum from latex concentratefactory (5%, v/v), macroelements andmicroelements, for 10 weeks at a room aeratedand illuminated by 20 W TL lamp at 50 cmdistance. Microelements were formulatedat a certain amount to give eleven combinationsof C: N: P: Mg. The Aiba & Ogawa syntheticmedium was used as a reference medium. Theoptimum growth of S. platensis had reachedafter eight-week incubation. Among elevenmedia composition containing latex serumexamined, best growth on a formulated mediumwith a ratio of C: N: P: Mg = 1:3:0.3:0.2 yielding0.350 g biomass/L This amount was slightlylesser than those on synthetic Aiba & Ogawamedium that yields 0.407 g biomass/L, aftereight-week incubation. Although the biomassproduction was lower than that of synthetic Aiba & Ogawa medium, the formulated mediagave higher carotenoid content. The highestcarotenoid content in biomass was 2.866 mg/kgbiomass obtained from a medium with ratio ofC: N: P: Mg = 1: 2: 0.3: 0. Thin layerchromatography (TLC) analysis of biomassextract showed the presence of two-six carotenoidcompounds, in which one of them is β- carotene.RingkasanSpirulina platensis adalah sianobakteria yangmenghasilkan berbagai senyawa bioaktif bernilaiekonomi tinggi antara lain karotenoida. Untukmemproduksi karotenoida dari biomassa selS. platensis secara efisien, perlu ditetapkankomposisi media mineral dan bahan organikkompleks yang optimal dari sumber nutrisi yangmurah. Spirulina platensis yang ditumbuhkandalam media serum lateks dari pabrik latekspekat dengan suplemen garam-garam mineraltertentu diharapkan produktif dalammenghasilkan karotenoida. Tujuan penelitianadalah menetapkan komposisi media yangoptimal untuk pertumbuhan dan produksikarotenoid serta mengidentifikasi jenis senyawakarotenoid dalam biomassa sel S. platensis.Sianobakteria ini ditumbuhkan dalam mediakompleks mengandung serum lateks pekat(5%, v/v) dengan suplemen nutrisi berupamakronutrien dan mikronutrien selama10 minggu di dalam ruangan dengan aerasi danpenyinaran lampu TL 20 W pada jarak 50 cm.Komposisi makronutrien diformulasi untukmemberikan sebelas macam variasi nisbahC:N:P:Mg. Sebagai pembanding digunakanmedia sintetik Aiba & Ogawa. Hasil penelitianmenunjukkan bahwa pertumbuhan S. platensismencapai puncak setelah diinkubasikan selamadelapan minggu. Dari 11 komposisi mediamengandung lateks yang diuji, pertumbuhanS. platensis terbaik adalah yang ditumbuhkandalam media formula dengan nisbah C:N:P:Mg=1:3:0.3:0.2 menghasilkan 0,350 g biomassa/L,sedikit lebih rendah dibandingkan denganmenggunakan media sintetik Aiba & Ogawa yangmenghasilkan 0,407 g biomassa/L selama8 minggu. Walaupun kandungan biomassanyalebih rendah, media formula tersebut meng-hasilkan karotenoid lebih tinggi dibandingkandengan media sintetik Aiba & Ogawa.Kandungan karotenoid tertinggi pada biomassayaitu sebesar 2.866 mg/kg diperoleh pada mediadengan nisbah C:N:P:Mg=1:2:0.3:0. Analisisekstrak biomassa dengan TLC menunjukkanadanya dua-enam jenis karotenoida, salahsatunya adalah β - karotena.

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Culture of Saccharomyces cerevisiae on hydrolyzed waste cassava starch for production of baking-quality yeast

Cited by 53 publications

References 5 publications

Fermentation optimization for the production of poly(β-hydroxybutyric acid) microbial thermoplastic

Fermentation optimization for the production of poly(β-hydroxybutyric acid) microbial thermoplastic

Pollutant removal-oriented yeast biomass production from high-organic-strength industrial wastewater: A review

Optimization media from low-cost nutrient sources for growing Spirulina platensis and carotenoid production Optimasi media dengan sumber nutrisi murah untuk pertumbuhan dan produksi karotenoid Spirulina platensis

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