Cultured Fish Species Affected by Red Sea Bream Iridoviral Disease from 1991 to 1995.

Matsuoka, Satoru; Inouye, Kiyoshi; Nakajima, Kazuhiro

doi:10.3147/jsfp.31.233

Cited by 83 publications

(54 citation statements)

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“…It is known that the iridoviral disease has afflicted 20 species of cultured marine fish in 17 prefectures located in the south-western part of Japan from 1991 to 1995. The infected fish include 18 Perciformes, 1 Pleuronectiformes and 1 Tetradontiformes (Matsuoka et al, 1996).…”

Section: ) Lymphocystis Diseasementioning

confidence: 99%

Viral Diseases in Cultured Marine Fish in Japan.

Nakajima¹,

Inouye²,

Sorimachi

1998

Fish Pathol.

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Section: ) Lymphocystis Diseasementioning

confidence: 99%

Viral Diseases in Cultured Marine Fish in Japan.

Nakajima¹,

Inouye²,

Sorimachi

1998

Fish Pathol.

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“…The causative agent was designated as red sea bream iridovirus (RSIV) (Nakajima and Sorimachi 1994). An early molecular epidemiologic investigation showed that RSIV infects over 30 marine-cultured fish in Japan (Kawakami and Nakajima 2002;Matsuoka et al 1996). Simultaneously, RSIV-like viral agents associated with mass mortality of finfish were also described from Australia (Anderson et al 1993), Singapore (Chua et al 1994), Chinese Taiwan (Chou et al 1998), mainland China (He et al 2000), South Korea (Jung and Oh 2000), and other Southeast Asian countries (Sudthongkong et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Cloning of a new fibroblast cell line from an early primary culture from mandarin fish (Siniperca chuatsi) fry for efficient proliferation of megalocytiviruses

et al. 2013

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Megalocytiviruses are important emerging pathogens in both freshwater and marine finfish aquaculture. However, a limited number of piscine cell lines are persistently susceptible to these viruses, which greatly limits the study of megalocytiviruses. In this study, a new fibroblast-like cell line was established from an early primary culture from mandarin fish fry by a single cell cloning and was designated as MFF-8C1. The MFF-8C1 cells grow well in Dulbecco's modified Eagle's medium supplemented with 10 % fetal bovine serum and had been subcultured more than 60 passages since the initial recovery culture in October 2009. Chromosomal analysis revealed that 91 % of the MFF-8C1 cells maintained a normal diploid chromosome number (2n = 48) in the 46th passage. Infection experiments showed that both freshwater-borne and marine-borne megalocytiviruses induce severe cytopathic effects in infected MFF-8C1 cells characterized by the rounding and enlargement of cells, which are highly consistent with the previous description of the infection in other susceptible cells with megalocytivirus. Megalocytivirus infections were further confirmed by a transmission electron microscopy. Furthermore, the MFF-8C1-cultured megalocytiviral suspension was highly virulent to infected mandarin fish. In summary, a new fibroblast cell line from mandarin fish fry that was highly permissive to megalocytiviruses was established. The MFF-8C1 cell line is a promising cellular substrate candidate for cell-cultured vaccine production of megalocytivirus.

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“…In growout stages of flounder, hirame rhabdovirus (HIRRV) infections (Kimura et al, 1986), red sea bream iridovirus (RSIV) infections (Matsuoka et al, 1996) and lympho cystis disease (Tanaka et al, 1984) have been reported.…”

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confidence: 99%

Isolation of Viruses from Wild Japanese Flounder Paralichthys olivaceus.

Takano¹,

Mori²,

Nishizawa³

et al. 2001

Fish Pathol.

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ABSTRACT-For a survey on fish pathogenic viruses among wild Japanese flounder Paralichthys olivaceus, 274 fish (body weight: 29-2240 g) were collected in 9 coastal areas of Japan in 1999 and 2000. Aquabirnaviruses were isolated, using RTG-2 and/or SSN-1 cells, from 111 fish (40.5%) caught in almost all the coastal areas and the representative isolate was identified as YTAV (yellow tail ascites virus) by cross-neutralization tests.Besides aquabirnaviruses, VHSV (viral hemor rhagic septicemia virus) was isolated, using EPC, SSN-1 and/or FHM cells, from 18 fish (6.6%) collected in 2 areas mainly in winter and spring. In a pathogenicity test, 4 isolates of aqua birnaviruses did not cause any mortality or morbidity in young Japanese flounder (average body weight 8.7 g) by intramuscular injection at 103.0-107.6 TCID50/ fish. Inoculation of an isolate of VHSV at 102.0 and 106.0TCID50 /fish produced mortalities of 60% and 100%, respectively, in flounder (24.8 g). Common external signs of flounder experimentally infected with VHSV were dark colora tion of the body with hemorrhagic fins and abdominal swelling, and the internal signs included ascites and extensive hemorrhage in the muscle and/or viscera.

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Cultured Fish Species Affected by Red Sea Bream Iridoviral Disease from 1991 to 1995.

Cited by 83 publications

References 1 publication

Viral Diseases in Cultured Marine Fish in Japan.

Viral Diseases in Cultured Marine Fish in Japan.

Cloning of a new fibroblast cell line from an early primary culture from mandarin fish (Siniperca chuatsi) fry for efficient proliferation of megalocytiviruses

Isolation of Viruses from Wild Japanese Flounder Paralichthys olivaceus.

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