Cultures from rat brain hemispheres enriched in oligodendrocyte-like cells

Labourdette, G.; Roussel, G.; Ghandour, Mohamedanwar; Nussbaum, J.L.

doi:10.1016/0006-8993(79)90508-0

Cited by 85 publications

(46 citation statements)

References 8 publications

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“…2) not only allowed us to confirm the previous reports (18,19) showing that OL grow better in high-than in low-density cultures, but also allowed us to add new insights to these observations. In fact, the experiments indicated that the larger number of OL present in high-density cultures is related to the proliferation of a larger proportion of LB1 + bipotential precursors (many of which are also 04+) and to a preferential differentiation of the precursors into OL, rather than into type-2 AS.…”

Section: Discussionsupporting

confidence: 79%

“…Primary cultures from 1-day-old Wistar rat cerebral cortex were prepared as described by Labourdette et al (19) with minor modifications. In brief, brain hemispheres stripped of meninges were dissociated through a 82-,m nylon mesh; cells were seeded onto poly(L-lysine)-coated 60-mm plastic dishes (two hemispheres per dish) and grown in BME/11o ( antigens, GalCer, and GFAP were carried out as described (6,9 …”

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confidence: 99%

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Differentiation of bipotential glial precursors into oligodendrocytes is promoted by interaction with type-1 astrocytes in cerebellar cultures.

Aloisi

Agresti

D’Urso

et al. 1988

Proc. Natl. Acad. Sci. U.S.A.

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The differentiation of bipotential precursors of oligodendrocytes (OL) and type-2 astrocytes (AS) was followed in primary cultures from 8-day postnatal rat cerebellum by labeling the cells with the antibodies LB1 (which binds to the surface disialoganglioside GD3 present in glial precur- "neuron-like" fashion (6,11), are sensitive to kainic acid (V. Gallo, R. Suergiu, C. Giovannini, and G.L., unpublished data), and express chondroitin sulfate (7). The bipotential cerebellar glial precursors pass through four distinct developmental stages during their differentiation into OL in serum-free cerebellar cultures (9). In the first stage, the cells are A2B5+ and LB1'. In the second stage, they can be stained also by the mAb 04, which binds to surface sulfatides and recognizes both immature and mature OL (12). In the third, short-lasting stage, the cells express on their surface also the well-established marker of OL, GalCer (13). In the fourth stage, however, the antigens recognized by A2B5 and LB1 are no longer detectable, and the cells remain positive for 04 and GalCer. Upon addition of FCS, the precursors maintain their ability to differentiate into type-2 AS until they are in the A2B5/LB1/04-positive stage (9). The factors responsible for the choice of the differentiation route taken by the precursor cells have not been defined. It (4,6) were resuspended in Eagle's basal medium (BME, GIBCO) supplemented with 2 mM glutamine, gentamycin (Sigma) at 0.1 mg/ml, and 10% heat-inactivated FCS (different batches from GIBCO); were seeded at 1 x 105 (low density) or 2.5 x 105 (high density) cells per cm2 onto

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Section: Discussionsupporting

confidence: 79%

mentioning

confidence: 99%

Differentiation of bipotential glial precursors into oligodendrocytes is promoted by interaction with type-1 astrocytes in cerebellar cultures.

Aloisi

Agresti

D’Urso

et al. 1988

Proc. Natl. Acad. Sci. U.S.A.

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“…Mixed primary glial cell cultures containing astrocytes and oligodendrocytes were prepared as previously described (Labourdette et al, 1979) with slight modifications (Feutz et al, 1995). Forebrains from newborn rat pups were teased with forceps and dissociated with a 10-ml syringe (18-gauge needle).…”

Section: Materials and Methods Primary Mixed Glial Cell Culturesmentioning

confidence: 99%

Oligodendrocytes express different isoforms of ?-amyloid precursor protein in chemically defined cell culture conditions: In situ hybridization and immunocytochemical detection

Garcia-Ladona¹,

Huss²,

Frey³

et al. 1997

J. Neurosci. Res.

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The expression of beta-amyloid precursor protein (betaAPP) by astrocytes is well documented; however, data concerning oligodendrocytes remain controversial. The main goal of the present study was to determine whether or not oligodendrocytes in culture constitutively express the different betaAPP isoforms. Oligodendrocytes were cultured in a chemically defined medium that avoids putative effects of unknown serum factors on oligodendrocyte development. We have employed immunocytochemistry and in situ hybridization with antibodies and synthetic oligonucleotides recognizing, respectively, specific protein epitopes and mRNA transcripts of rat betaAPP isoforms. Oligodendrocytes, in both mixed primary cultures in the presence of serum or in secondary cultures in defined medium, were clearly labeled by antibodies directed to different betaAPP sequences. Antibodies against the serine protease inhibitor domain of betaAPP, also strongly labelled oligodendrocytes. Immunohistochemistry and in situ hybridization were combined to determine precisely the expression of different isoforms of betaAPP. In situ hybridization revealed the presence in oligodendrocytes of mRNA transcripts coding not only for betaAPP695 but also for betaAPP770 and betaAPP751. This indicates that betaAPP immunoreactivity found in oligodendrocytes corresponds to constitutive expression of betaAPP. Oligodendrocyte cultured in chemically defined medium are able to express not only betaAPP695 but also betaAPP770, betaAPP751 isoforms containing the Kunitz protease inhibitor domain. Although the role of betaAPP in the pathological processes of Alzheimer's disease (AD) remains unknown, possible disturbances of betaAPP processing and/or synthesis in oligodendrocytes may account for some myelin disorders observed in AD and other senile dementias.

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“…Secondary OL cultures were prepared from newborn Sprague-Dawley rat brains by modification of the methods described by Besnard et al (1987Besnard et al ( , 1989 and Labourdette et al (1979Labourdette et al ( , 1980. Primary mixed glial cultures were grown in Waymouth's medium (Gibco, Europe SA) supplemented with penicillin (50 U/ml), streptomycin (50 mg/ml), sodium bicarbonate (2 g/L), sodium pyruvate (110 mg/ml), and 10% donor calf serum (Gibco).…”

Section: Cell Culturesmentioning

confidence: 99%

Rat oligodendrocytes express the vitamin D3 receptor and respond to 1,25-dihydroxyvitamin D3

Baas

Prüfer

Ittel

et al. 2000

Glia

127

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Cultures from rat brain hemispheres enriched in oligodendrocyte-like cells

Cited by 85 publications

References 8 publications

Differentiation of bipotential glial precursors into oligodendrocytes is promoted by interaction with type-1 astrocytes in cerebellar cultures.

Differentiation of bipotential glial precursors into oligodendrocytes is promoted by interaction with type-1 astrocytes in cerebellar cultures.

Oligodendrocytes express different isoforms of ?-amyloid precursor protein in chemically defined cell culture conditions: In situ hybridization and immunocytochemical detection

Rat oligodendrocytes express the vitamin D3 receptor and respond to 1,25-dihydroxyvitamin D3

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