Cupressus lusitanica (Cupressaceae) leaf extract induces apoptosis in cancer cells

López, Laura Zufía; Villavicencio, Miguel Angel; Albores, Arnulfo; Martinez, Macario; Garza, Jaime de la; Meléndez-Zajgla, Jorge; Maldonado, Vilma

doi:10.1016/s0378-8741(01)00417-2

Cited by 19 publications

(9 citation statements)

References 14 publications

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“…2). Translocation of membrane phosphatidylserine and sub-G1 fraction is a manifestation of programmed cell death that occurs naturally in cells, and can be beneficial to cancer therapy [32]. The presence of phosphatidylserine on the outer leaflet of apoptotic cells membrane was assessed using Annexin V-cy3/6CFDA staining to quantify the amount of cells in the early stage of apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Furano-sesquiterpene from soft coral, Sinularia kavarittiensis: induces apoptosis via the mitochondrial-mediated caspase-dependent pathway in THP-1, leukemia cell line

et al. 2009

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Bioassay directed fractionation and purification led to the successful isolation of a furano sesquiterpene, Methyl 5-[(1E,5E)-2,6-Dimethyl octa-1,5,7-trienyl] furan-3-carboxylate (MDTFC), a bioactive component from a soft coral, Sinularia kavarittiensis. Its structure was determined by analyzing (1)H, (13)C NMR and FAB-MS. The results show that MDTFC could efficiently and selectively inhibit the proliferation of several human cancer cell lines. Among all the cell lines, THP-1 was found to be most sensitive (IC(50) 29.59 microM), whereas the peripheral blood mononuclear cells were least effected (IC(50) 464.16 microM). The molecular mechanism of MDTFC mediated apoptosis was investigated for the first time. Induction of apoptosis in THP-1 cells was characterized by cell membrane blebbing, chromatin condensation, DNA fragmentation, and decrease in level of pro-caspases 3, 9 and increase in Bax/Bcl-2 ratio. Our results were further strengthened through cleavage of poly (ADP-ribose) polymerase, reduction of mitochondrial membrane potential (Psim) and cytosolic release of cytochrome c, which are key events during apoptosis. Moreover, phosphatidyl serine exposure and appearance of sub-G1 peak also demonstrated cell death, when analyzed by flow cytometry. DNA fragmentation was prevented moderately when pretreated with caspase-9 inhibitor (Z-LEHD-FMK) and largely with caspase-3 inhibitor (Z-DEVD-FMK). In summary, MDTFC mediated apoptosis involves mitochondria-dependent pathway and the present compound of marine origin might have a therapeutic value against human cancer cell lines and especially on leukemia cells.

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Section: Discussionmentioning

confidence: 99%

Furano-sesquiterpene from soft coral, Sinularia kavarittiensis: induces apoptosis via the mitochondrial-mediated caspase-dependent pathway in THP-1, leukemia cell line

et al. 2009

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“…Cell death is likely to be apoptotic, or due to morphological changes like cell shrinkage, membrane blebbing, chromatin condensation, and nuclear fragmentation with formation of apoptotic bodies. The apoptotic cell death that naturally occurs in cells may benefit cancer therapy development []. Sinularin is an active compound isolated from the soft coral Sinularia flexibilis .…”

Section: Introductionmentioning

confidence: 99%

Proteomic investigation of anti‐tumor activities exerted by sinularin against A2058 melanoma cells

Su¹,

Lin

Chiu

et al. 2012

Electrophoresis

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The extracts from soft corals have been increasingly investigated for biomedical and therapeutic purposes. The aim of this study is to examine and analyze the anti-tumor effects of the genus Sinularia extract sinularin on A2058 melanoma cells using MTT assay, cell migration assay, wound healing assay, flow cytometric analysis, and proteomic analysis. Sinularin dose-dependently (1-5 μg/mL) inhibited melanoma cell proliferation while the treatment at identical concentrations suppressed cell migration. Sinularin dose-dependently enhanced apoptotic melanoma cells and caused tumor cell accumulation at G2/M phase, indicating that sinularin exerts apoptosis-induced and cell cycle-delayed activities in A2058 melanoma cells. Comparative proteomic analysis was conducted to investigate the effects of sinularin at the molecular level by comparison between the protein profiling of melanoma cells treated with sinularin and without the treatment. Thirty-five differential proteins (13 upregulated and 22 downregulated) concerning the treatment were identified by liquid chromatography-tandem mass spectrometry. Proteomic data and Western blot displayed the levels of several tumor inhibitory or apoptosis-associated proteins including annexin A1, voltage-dependent anion-selective channel protein 1 and prohibitin (upregulated), heat shock protein 60, heat shock protein beta-1, and peroxiredoxin-2 (downregulated) in A2058 melanoma cells exposed to sinularin. Increased expression of p53, cleaved-caspase-3, cleaved-caspase-8, cleaved-caspase-9, p21, and Bax and decreased expression of Bcl-2 in sinularin-treated melanoma cells suggest that the anti-tumor activities of sinularin against melanoma cells are particularly correlated with these pro-apoptotic factors. These data provide important information for the mechanisms of anti-tumor effects of sinularin on melanoma cells and may be helpful for drug development and progression monitoring of human melanoma.

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“…Cell death can be apoptotic, or result from morphological changes such as membrane blebbing, cell shrinkage, chromatin condensation, and nuclear fragmentation with formation of apoptotic bodies. Translocation of membrane phosphatidylserine and sub-G 1 fraction is a form of programmed cell death that occurs naturally in cells and can be beneficial to cancer therapy [14]. Ability to manipulate the machinery of cell death is an obvious goal of medical research, and effect on regulation of apoptosis might lead to new possibilities for oral cancer treatment [15].…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic Effect of the GenusSinulariaExtracts on Human SCC25 and HaCaT Cells

Wang

Chou

Lin

et al. 2009

Journal of Toxicology

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Soft corals of the genus Sinularia are being increasingly adopted to treat a wide variety of disease processes. However, the mechanism underlying its activity against human oral cancer cells is poorly understood. This study evaluates the cyototoxicity effects of the genus Sinularia extracts (S. grandilobata, S. parva, S. triangula, S. scabra, S. nanolobata and S. gibberosa) by SCC25 and HaCaT cells. The cell adhesion assay indicates that extracts reduce the cell attachment. Extracts exhibit a dose-dependent cytotoxic effect using MTS assay.Treatment of extracts to observe the morphological alterations in cells, membrane blebbing, nuclear condensation, and apoptotic bodies is demonstrated. Flow cytometry shows that extracts sensitized the cells in the G0/G1 and G2/M phases with a concomitant significantly increased sub-G1 fraction, suggesting cell death by apoptosis. Extracts of the genus Sinularia thus apparently cause apoptosis of SCC25 and HaCaT cells, and warrant further research investigating the possible antioral cancer compounds in these soft corals.

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Cupressus lusitanica (Cupressaceae) leaf extract induces apoptosis in cancer cells

Cited by 19 publications

References 14 publications

Furano-sesquiterpene from soft coral, Sinularia kavarittiensis: induces apoptosis via the mitochondrial-mediated caspase-dependent pathway in THP-1, leukemia cell line

Furano-sesquiterpene from soft coral, Sinularia kavarittiensis: induces apoptosis via the mitochondrial-mediated caspase-dependent pathway in THP-1, leukemia cell line

Proteomic investigation of anti‐tumor activities exerted by sinularin against A2058 melanoma cells

Cytotoxic Effect of the GenusSinulariaExtracts on Human SCC25 and HaCaT Cells

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