Curative and hepatoprotective action of fenugreek (Trigonellafoenumgaraecum) seeds against cisplatin induced oxidative liver damage in male albino rats

Abstract

Fenugreek is one of several herbal medicines whose seeds and leaves are used either as food or as drink. The present study was designed to investigate possible potential hepatocurative & hepatoprotective activity of 5% and 10% of fenugreek (Trigonellafoenumgaraecum) seeds against cisplatin (5mg/kg body wt.) induced oxidative liver damage in male albino rats. The experimental protocol designed as the animals(42 rats) were divided into seven groups including both curative and protective groups (six rats each) : Group (1) Negative control group was fed on basal diet only for 28 days and curative groups including subgroups (2,3,4) were injected interaperitoneal by cisplatin (5mg/kg body wt.) from the first day and waiting for 24 hours to induce hepatotoxicity as follows: curative Positive control group (subgroup 2) was fed on basal diet after injection, subgroup (3,4) was fed basal diet +5% and 10% fenugreek seeds powder respectively after injection by cisplatin. While Protective groups including subgroups (5,6,7), they were injected with cisplatin interaperitoneal on the last day of the experiment and kept 24 hours after injection and divided as follows : protective Positive control group (subgroup 5) was fed basal diet and given cisplatin interaperitoneal injection (5mg/kg body wt.) on the last day of experiment. Subgroups ( 6) and ( 7) were fed basal diet and given fenugreek seeds 5% and 10% respectively and they were injected with cisplatin interaperitoneal on the last day of the experiment , then all rats were fasted overnight (12 hours), sacrificed and blood samples were collected to determine chemical analysis (AST, ALT, ALP, total protein, albumin, globulin, Albumin/Globulin, MDA, SOD, CAT. Besides, nutritional and biological parameters were recorded (FI, BWG%, FER). The results showed that fenugreek seeds powder at 5% and 10% intake in curative and protective groups improved feed intake, the body weight gain, feed efficiency ratio, liver function, and antioxidant enzymes compared with positive control groups (injected with cisplatin). It can be concluded that fenugreek seed improved the activities of liver functions and antioxidant enzymes against cisplatin that induced oxidative liver injury.