Curcumin alleviated lipopolysaccharide‐induced lung injury via regulating the Nrf2‐ARE and NF‐κB signaling pathways in ducks

Abstract

BACKGROUND Under the intensive modern poultry farming system, the lung of duck is one of the main target organs for various bacterial and viral infections. Curcumin is a kind of natural polyphenol compound for which various beneficial biological functions exist, including being an anti‐inflammatory, antioxidant, and antiviral. The aim of this work was to investigate the mechanism of curcumin‐alleviated lipopolysaccharide (LPS)‐induced lung damage by the nuclear erythroid 2‐related factor 2 (Nrf2)‐antioxidant reaction element (ARE) and nuclear factor kappa B (NF‐κB) signaling pathway in ducks. RESULTS In total, 450 one‐day‐old male specific pathogen‐free ducks were randomly assigned into three dietary treatments: CON, basal diet; LPS, basal diet + LPS treatment; LPS + CUR, basal diet + LPS + 500 mg kg−1 of curcumin. At the end of the experiment (21 days), ducks in LPS treatment were challenged with 5 mg LPS per kilogram of body weight and the other two treatments were injected with the same dose of phosphate‐buffered saline solution. The results showed that LPS caused acute inflammation, oxidation stress, and lung injury. Dietary addition of curcumin significantly relieved the oxidation stress and inflammation parameters. Moreover, the results showed that remission may be through the signaling pathways of both Nrf2‐ARE and NF‐κB. CONCLUSION In conclusion, dietary supplementation of 500 mg kg−1 of curcumin exhibited a lung‐protective effect in ducks. This experiment broadens the mode of metabolism actions of curcumin in the target organs and provides an insight for the application of curcumin in waterfowl feed. © 2022 Society of Chemical Industry.

Curcumin Mitigates Oxidative Damage in Broiler Liver and Ileum Caused by Aflatoxin B1-Contaminated Feed through Nrf2 Signaling Pathway

“…Previous studies have shown that 1 mg/kg of AFB1 can lead to inflammatory liver damage in broilers [28], and in our previous study, the addition of 500 mg/kg of CUR to the feed inhibited oxidative stress in the liver and gut of poultry [21]. In this study, we investigated the damage to the liver and ileum of broilers caused by 1 mg/kg AFB1contaminated diets, as well as the protective effect of 500 mg/kg CUR in diets, and focused on the role that the Nrf2 signaling pathway plays.…”

“…CUR stimulates antioxidant enzyme activity, scavenges free radicals, inhibits ROS generation, blocks lipid peroxidation of cell membranes, and up-regulates antioxidant gene expression through the Nrf2 transcription factor, which results in the enhancement of the antioxidant functions of animals [17]. More importantly, studies have shown that CUR plays an important antioxidant role in protecting poultry health [18][19][20][21]. CUR has been found to improve the growth performance of broilers by enhancing intestinal morphology, subsequently affecting feed digestibility [22,23].…”

Curcumin as a Potential Antioxidant in Stress Regulation of Terrestrial, Avian, and Aquatic Animals: A Review

“…On the other hand, lipopolysaccharides (LPSs), components responsible for the pathological process of contamination induced by Gram-negative bacteria, can cause morphological damage to the ileum, lung injury, inflammation, and oxidative stress in poultry. The addition of 500 mg/kg of curcumin to the diet could ameliorate these symptoms in the lungs and ileitis of ducks through the signaling pathways of Nrf2-ARE, NF-κB, and TLR/NF-κB in an orderly manner [ 87 , 88 ]. Furthermore, curcumin has shown effectiveness against coccidiosis caused by Eimeria species.…”

Echinacea Polyphenols Inhibit NLRP3-Dependent Pyroptosis, Apoptosis, and Necroptosis via Suppressing NO Production during Lipopolysaccharide-Induced Acute Lung Injury

“…Accumulating evidences indicate that the interactions between Echinacea purpurea and cells may involve different compounds . Echinacea polyphenols (EPP) is viewed as the most active compound of Echinacea purpurea. − Lipopolysaccharide (LPS) is an important constituent of the outer membrane of Gram-negative bacteria, and it is well known as an inducer of ALI model. , However, the protective effect of EPP on LPS-induced ALI, and the underlying mechanisms associated with PANoptosis need to be further explored. Thus, our study aimed to systematically explore the protective effect of EPP against the LPS-induced ALI and the potential mechanisms both in vitro and in vivo , so as to provide a new idea for the prevention and control of ALI, and establish a theoretical foundation for further research of Echinacea purpurea.…”