Curcumin and Silibinin Inhibit Telomerase Expression in T47D Human Breast Cancer Cells

Nasiri, Marzieh; Zarghami, Nosratollah; Nejati-Koshki, Kazem; Mollazadeh, Mahdieh; Pourhassan‐Moghaddam, Mohammad; Yamchi, Mohammad Rahmati; Esfahlan, Rana Jahanban; Barkhordari, Amin; Alibakhshi, Abbas

doi:10.7314/apjcp.2013.14.6.3449

Cited by 71 publications

(58 citation statements)

References 25 publications

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“…Moreover, Meiyanto, et al 2006, reported that PGV-0 possesses cytotoxic activity to T47D cells with the IC 50 of 10 μM , whereas the IC 50 value of PGV-1 in T47D breast cancer cells is 3.16 μM, which is more toxic than curcumin (IC 50 =19.05 µM). In addition, modification on curcumin compound possibly improves inhibitory effect of some protein kinases, such as Pgp, EGFR and HER2 thus will be more potential as chemopreventive agents (Nasiri, 2013). Therefore, further research needs to be explored in specific target especially in the application as co-chemotherapeutic agents with Dox for breast cancer.…”

Section: Introductionmentioning

confidence: 99%

Curcumin and its Analogues (PGV-0 and PGV-1) Enhance Sensitivity of Resistant MCF-7 Cells to Doxorubicin through Inhibition of HER2 and NF-kB Activation

Meiyanto¹,

Putri²,

Susidarti³

et al. 2014

Asian Pacific Journal of Cancer Prevention

113

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Section: Introductionmentioning

confidence: 99%

Curcumin and its Analogues (PGV-0 and PGV-1) Enhance Sensitivity of Resistant MCF-7 Cells to Doxorubicin through Inhibition of HER2 and NF-kB Activation

Meiyanto¹,

Putri²,

Susidarti³

et al. 2014

Asian Pacific Journal of Cancer Prevention

113

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“…Cooperative medication of two or more drugs results in synergism among the different drugs against cancer cells and can conquer Mehran Mesgari Abbasi et al drug resistance through distinct mechanisms of action (Nasiri et al, 2013;Valiyari S et al, 2013). On the other side, nanoparticle drug delivery enhances therapeutic effectiveness whilst reduces toxicity on normal cells and vital organs by ameliorating their pharmacokinetics and bioavalibilities.…”

Section: Introductionmentioning

confidence: 99%

DOX-MTX-NPs Augment p53 mRNA Expression in OSCC Model in Rat: Effects of IV and Oral Routes

Abbasi¹,

Khiavi²,

Amir³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Background: Oral squamous cell carcinoma (OSCC) is the sixth most common malignancy worldwide. Cancer development and progression require inactivation of tumor suppressor genes and activation of proto-oncogenes. The well recognized mechanism of action demonstrated for chemotherapeutic agents is induction of apoptosis via reactivation of p53. In this context, we evaluate the efficacy of IV and oral routes of our novel PH and temperature sensitive doxorubicin-methotrexate-loaded nanoparticles (DOX-MTX NP) in affecting p53 profile in an OSCC rat model. Methods: In this study, 120 male rats were divided into 8 groups of 15 animals each. The new formulated DOX-MTX NP and free doxorubicin were IV and orally given to rats with 4-nitroquinoline-1-oxide induced OSCC. Results: Results showed that both DOX and DOX-MTX-NP caused significant increase in mRNA levels of P53 compared to the untreated group (p<0.000). With both DOX and DOX-MTX NP, the IV mode was more effective than the oral (gavage) route (p<0.000). Surprisingly, in oral mode, p53 mRNA was not affected in DOX treated groups (p>0.05), Nonetheless, both IV and oral administration of MTX-DOX NP showed superior activity (~3 fold) over free DOX in reactivation of p53 in OSCC (p<0.000). The effectiveness of oral route in group treated with nanodrug accounts for the enhanced bioavailability of nanoparticulated DOX-MTX compared to free DOX. Moreover, in treated groups, tumor stage was markedly related to the amount of p53 mRNA (p<0.05). Conclusion: Both oral and IV application of our novel nanodrug possesses superior activity over free DOX-in up-regulation of p53 in a OSCC model and this increase in p53 level associated with less aggressive tumors in our study. Although, impressive results obtained with IV form of nanodrug (-21 fold increase in p53 mRNA level) but both forms of nanodrug are effective in OSCC, with less toxicity normal cells.

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“…In this study, we used β-Cyclodextrin-Helenalincomplex nanoparticles to inhibit T47D breast cancer cell lines. To show the efficiency of our nanoparticle, we compared our results (T47D cell lines treated with β-Cyclodextrin-Helenalincomplex) with Nasiri et al (2013) experiments that they studied the effect of pure Helenalin on T47D cell lines. Our experiments demonstrated that when we treat cell lines with the same values of β-Cyclodextrin-Helenalin complex and Helenalin-free, under the same conditions, β-Cyclodextrin-Helenalin complex is more effective and kill some more breast cancer cells.…”

Section: Discussionmentioning

confidence: 99%

“…Studies showed that telomerase is over expressed in about 85-90% of human breast cancer cells (Herbet et al, 2001;Kirkpatrick et al, 2003;Torkani and Aradi, 2008) RNA dependent DNA polymerase that synthesize the repetitive nucleotide sequence (TTAGGG in human beings) at telomeres which are the regions of repetitive nucleotide sequences at each end of a chromosome with protective effect on the end of linear chromosomes and avoid decline or fusion with adjacent chromosomes (Mokbel, 2000;Nasiri, 2013). During cell division, DNA polymerase is not capable to fully replicate the ends of linear DNA and consequently genetic material is lost from chromosomal ends (Mokbel, 2000;Kirkpatrick et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Effects of β-Cyclodextrin-Helenalin Complexes on H-TERT Gene Expression in the T47D Breast Cancer Cell Line - Results of Real Time Quantitative PCR

Ghasemali

Nejati-Koshki²,

Tafsiri³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Background: Nowadays, the encapsulation of cytotoxic chemotherapeutic agents is attracting interest as a method for drug delivery. We hypothesized that the efficiency of helenalin might be maximized by encapsulation in β-cyclodextrin nanoparticles. Helenalin, with a hydrophobic structure obtained from flowers of Arnica chamissonis and Arnica Montana, has anti-cancer and anti-inflammatory activity but low water solubility and bioavailability. β-Cyclodextrin (β-CD) is a cyclic oligosaccharide comprising seven D-glucopyranoside units, linked through 1,4-glycosidic bonds. Materials and Methods: To test our hypothesis, we prepared β-cyclodextrinhelenalin complexes to determine their inhibitory effects on telomerase gene expression by real-time polymerase chain reaction (q-PCR) and cytotoxic effects by colorimetric cell viability (MTT) assay. Results: MTT assay showed that not only β-cyclodextrin has no cytotoxic effect on its own but also it demonstrated that β-cyclodextrinhelenalin complexes inhibited the growth of the T47D breast cancer cell line in a time and dose-dependent manner. Our q-PCR results showed that the expression of telomerase gene was effectively reduced as the concentration of β-cyclodextrin-helenalin complexes increased. Conclusions: β-Cyclodextrin-helenalin complexes exerted cytotoxic effects on T47D cells through down-regulation of telomerase expression and by enhancing Helenalin uptake by cells. Therefore, β-cyclodextrin could be superior carrier for this kind of hydrophobic agent.

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Curcumin and Silibinin Inhibit Telomerase Expression in T47D Human Breast Cancer Cells

Cited by 71 publications

References 25 publications

Curcumin and its Analogues (PGV-0 and PGV-1) Enhance Sensitivity of Resistant MCF-7 Cells to Doxorubicin through Inhibition of HER2 and NF-kB Activation

Curcumin and its Analogues (PGV-0 and PGV-1) Enhance Sensitivity of Resistant MCF-7 Cells to Doxorubicin through Inhibition of HER2 and NF-kB Activation

DOX-MTX-NPs Augment p53 mRNA Expression in OSCC Model in Rat: Effects of IV and Oral Routes

Inhibitory Effects of β-Cyclodextrin-Helenalin Complexes on H-TERT Gene Expression in the T47D Breast Cancer Cell Line - Results of Real Time Quantitative PCR

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