Curcumin Derivative MTH-3 Regulates Palmitate-induced Insulin Resistance in Mouse Myoblast C₂C₁₂Cells

Abstract

Background/Aim: At present, there are no effective drugs for the treatment of insulin resistance. MTH-3, a curcumin derivative, exerts potent anti-cancer effects. The aim of the present study was to explore whether MTH-3 is capable of regulating palmitic acid (PA)-induced insulin resistance in C 2 C 12 cells. Materials and Methods: Cell viability was examined using the MTT assay. C 2 C 12 cells were treated with PA and evaluated for the production of oil droplets using an Oil Red O assay. Glucose uptake was analysed by the 2-NBDG assay. Results: Treatment of cells with up to 500 μM PA for 24 h or with 5 or 10 μM MTH-3 had no effect on cell viability. PA induced production of oil droplets in C 2 C 12 cells. After adding MTH-3, the quantity of oil droplets decreased significantly and glucose uptake recovered. Conclusion: MTH-3 may become an efficient drug for the treatment of insulin resistance and associated diseases.

Effect of Formulation Variables and Bile Salts Addition on Entrapment Efficiency of Curcumin Loaded Niosomes

“…The Curcumin (C21H20O6) is a natural yellow compound typically found in Curcuma longa L. that is regarded as a natural polyphenolic antioxidant presented in many kinds of herbs 8 . Curcumin has been exhibited multiple therapeutic relevance including anticancer, antiinflammatory, antioxidant, antimicrobial, antirheumatic, and hepatoprotective activities [9][10][11][12][13] . Its anticancer, antiinflammatory, antiangiogenic, antineoplastic, and chemo sensitizing effects make it a potent candidate in the treatment for multiple types of cancer 14,15 .…”