Curcumin in Combination with Piperine Suppresses Osteoclastogenesis In Vitro

Martins, Caroline Alberici; Leyhausen, Gabriele; Volk, Joachim; Geurtsen, Werner

doi:10.1016/j.joen.2015.05.009

Cited by 31 publications

(20 citation statements)

References 34 publications

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“…22) It has been demonstrated that curcumin combined with piperine suppressed the osteoclastogenesis. 23) In this study, we found that curcumin had no effect on TGF-β3 induced chondrogenesis of MSCs. Several chondrogenic genes such as Sox9 and Col2a1 were not changed on the molecular level.…”

Section: Discussionmentioning

confidence: 57%

Curcumin Inhibits Chondrocyte Hypertrophy of Mesenchymal Stem Cells through IHH and Notch Signaling Pathways

Cao

Dou

Dong

2017

CHEMICAL & PHARMACEUTICAL BULLETIN

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Using tissue engineering technique to repair cartilage damage caused by osteoarthritis is a promising strategy. However, the regenerated tissue usually is fibrous cartilage, which has poor mechanical characteristics compared to hyaline cartilage. Chondrocyte hypertrophy plays an important role in this process. Thus, it is very important to find out a suitable way to maintain the phenotype of chondrocytes and inhibit chondrocyte hypertrophy. Curcumin deriving from turmeric was reported with anti-inflammatory and antitumor pharmacological effects. However, the role of curcumin in metabolism of chondrocytes, especially in the chondrocyte hypertrophy remains unclear. Mesenchymal stem cells (MSCs) are widely used in cartilage tissue engineering as seed cells. So we investigated the effect of curcumin on chondrogenesis and chondrocyte hypertrophy in MSCs through examination of cell viability, glycosaminoglycan synthesis and specific gene expression. We found curcumin had no effect on expression of chondrogenic markers including Sox9 and Col2a1 while hypertrophic markers including Runx2 and Col10a1 were down-regulated. Further exploration showed that curcumin inhibited chondrocyte hypertrophy through Indian hedgehog homolog (IHH) and Notch signalings. Our results indicated curcumin was a potential agent in modulating cartilage homeostasis and maintaining chondrocyte phenotype.Key words curcumin; mesenchymal stem cell; chondrocyte hypertrophy; Indian hedgehog homolog; neurogenic locus notch homolog Osteoarthritis (OA) is a degenerative joint disease accompanied by pain and disability.1) Seventy percent of the population older than 65 years is suffering from varying degrees of OA.2)The pathogenesis of cartilage injury caused by OA is the disruption of the homeostasis between catabolism and anabolism of extracellular matrix.3) However, limited by the intrinsic regeneration capacity of cartilage tissue, repairing damaged articular cartilage currently is a significant challenge. 4,5) With the advances in materials science, cell biology, biomechanics, using tissue engineering technology to construct cartilage provides a novel method for repairing damaged cartilage.6) Mesenchymal stem cells (MSCs) have potential of differentiation into chondrocytes and have got the priority in the seed cells choosing list because of their traits in rich resources, easy to get and low immunogenicity. 7,8) However, the regenerated tissue-engineered cartilage usually is fibrous cartilage rather than hyaline cartilage.9) The chondrocytes in fibrous cartilage proceed in hypertrophic differentiation and secret Col10a1 and matrix metalloproteinase 13 (Mmp13).10) Therefore, suppressing chondrocyte hypertrophy is the key issue to build a stable hyaline cartilage.Curcumin is effective substance of turmeric and possesses various pharmacological effects, including antioxidant, anti-bacterial, anti-tumor and anti-inflammatory.11-13) A recent study has reported that curcumin may be a potential therapeutic candidate for Helicobacter pylori associated diseas...

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Section: Discussionmentioning

confidence: 57%

Curcumin Inhibits Chondrocyte Hypertrophy of Mesenchymal Stem Cells through IHH and Notch Signaling Pathways

Cao

Dou

Dong

2017

CHEMICAL & PHARMACEUTICAL BULLETIN

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“…7,8 In recent years, compounds of natural origin, obtained from plants such as curcumin and piperine, have been tested to formulate anti-inflammatory and antiosteoclastogenic drugs having the potential to treat and prevent replacement resorption of replanted teeth. 9 In this respect, a recent study tested the effect of a plant lectin on stimulated gingival fibroblasts, with promising results 10 for future use as a possible therapy for delayed replanted teeth. Lectins are carbohydrate-binding proteins involved in various biological process, including cell-cell recognition, proliferation, migration, adhesion and biochemical responses.…”

Section: Introductionmentioning

confidence: 99%

Effect of ScLL and 15d-PGJ2 on viability and cytokine release in LPS-stimulated fibroblasts: an in vitro study

et al. 2020

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This study evaluated the effect of a cyclopentenone-type PG, 15-Deoxy-Δ12,14 -PG J2 (15d-PGJ 2 ), and lectin (ScLL) on the viability of human gingival fibroblasts (HGFs), and on IL-6 and TGFβ-1 release by these fibroblasts, stimulated with lipopolysaccharide (LPS). HGFs were stimulated with LPS 10 μg/ml and treated with 15d-PGJ 2 1 and 2 μg/ml, and ScLL 2 and 5 μg/ml, for 1 and 3h, and then evaluated for viability by MTT assay. Supernatant was collected to detect IL-6 and TGFβ-1 release, by ELISA. Positive control was cells kept in Dulbecco's Modified Eagle's Medium, and negative control was those kept in LPS. Data were analyzed by ANOVA and Dunnett's test (α = 0.05). No significant difference was found in viability among experimental groups at 1h (p > 0.05). Percentage of ScLL 5 µg/ml viable cells was similar to that of positive control at evaluated periods (p > 0.05), whereas the other groups had lower levels than the positive control (p < 0.05). IL-6 release was statistically higher for ScLL 5 μg/ml and 15d-PGJ 2 2 µg/ml at 1h, compared with the other treated groups and positive control (p < 0.05). No significant differences were found among the groups at 3h (p > 0.05), except for ScLL 2 µg/ml and 15d-PGJ 2 1 µg/ml, which showed lower IL-6 release compared with that of negative control (p < 0.05). No significant difference was found among the groups for TGFβ-1 release (p > 0.05). Results indicated that ScLL 5 μg/ml did not interfere in viability, and ScLL 2 µg/ml and 15d-PGJ 2 1 µg/ml demonstrated reduced IL-6 release. Tested substances had no effect on TGFβ-1 release.

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“…Considering the difficulty in isolating and differentiating primary cultures of osteoclasts, and also the fact that RAW264.7 cells are widely used as a cellular model in studies involving osteoclastogenesis, they were used to evaluate the effects of ScLL on osteoclast‐like precursors. However, the use of RAW 264.7 has limitations because it presents characteristics that may resemble multinucleated osteoclasts or multinucleated giant cells.…”

Section: Discussionmentioning

confidence: 99%

“…The osteoclast-like cells obtained by RAW 264.7 cell differentiation express the hallmark characteristics of human osteoclasts in vivo, with representative properties including TRAP and cathepsin K. 13,19 The activity of TRAP is based on a resorptive enzyme found in the membrane of osteoclasts, and the rate of activity is proportional to the synthesis and secretion of the enzyme. 20 Cathepsin K is a lysosomal cysteine protease localized to the ruffled border in actively resorbing osteoclasts, where it degrades the two main types of collagen, I and II, within the acidic microenvironment of the resorption lacunae. 24 Furthermore, the activity of cathepsin K is essential to the initiation of actin ring formation and the activation of osteoclasts.…”

Section: Discussionmentioning

confidence: 99%

Effects of Lectin (ScLL) on osteoclast‐like multinucleated giant cells’ maturation—A preliminary in vitro study

Reis

Souza

Moura

et al. 2018

Dental Traumatology

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Curcumin in Combination with Piperine Suppresses Osteoclastogenesis In Vitro

Cited by 31 publications

References 34 publications

Curcumin Inhibits Chondrocyte Hypertrophy of Mesenchymal Stem Cells through IHH and Notch Signaling Pathways

Curcumin Inhibits Chondrocyte Hypertrophy of Mesenchymal Stem Cells through IHH and Notch Signaling Pathways

Effect of ScLL and 15d-PGJ2 on viability and cytokine release in LPS-stimulated fibroblasts: an in vitro study

Effects of Lectin (ScLL) on osteoclast‐like multinucleated giant cells’ maturation—A preliminary in vitro study

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