Curcumin inhibits heat-induced oxidative stress by activating the MAPK-Nrf2 / ARE signaling pathway in chicken fibroblasts cells

Wu, Jianli; Ibtisham, Fahar; Niu, Yan; Wang, Zhi; Li, Guang Hui; Yun-feng, Zhao; Nawab, Aamir; Xiao, Mei; An, Lilong

doi:10.1016/j.jtherbio.2018.12.004

Cited by 47 publications

(36 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This result was in agreement with a previous study which found that the upregulation of Nrf2 mRNA might be explained by the high expression of TOR (23). A study on cows reported the upregulation of p38 MAPK mRNA levels with decreasing Nrf2 mRNA levels in the mammary gland (67), which supported our findings in current study. Overall, 2.44% lysine supplementation could improve the intestinal antioxidant capacity of grass carp fry via the TOR, p38 MAPK and Nrf2 signaling pathways; however, the mechanism of by which dietary lysine regulates the TOR/Nrf2 and p38 MAPK/Nrf2 signaling pathways in grass carp fry is still unclear and requires further investigation.…”

Section: Discussionsupporting

confidence: 94%

Dietary Lysine Levels Improved Antioxidant Capacity and Immunity via the TOR and p38 MAPK Signaling Pathways in Grass Carp, Ctenopharyngodon idellus Fry

et al. 2021

View full text Add to dashboard Cite

An 8-week rearing trial was designed to appraise the dietary lysine levels on intestinal antioxidant capacity and immunity of grass carp fry. Six practical diets were prepared with graded levels of lysine (1.44, 1.79, 1.97, 2.44, 2.56 and 2.87% dry matter), and these diets were fed to grass carp fry. The results showed that the activities of intestinal antioxidant factors including catalase and glutathione peroxidase were markedly improved by the 2.44% dietary lysine compared with the control diet (1.44% dietary lysine) (P < 0.05). In terms of antioxidants, compared with the control diet, the 2.44% diet markedly upregulated the mRNA expression levels of target of rapamycin, S6 kinase1 and nuclear factor erythroid 2-related factor 2 pathway-related antioxidant genes, containing catalase and glutathione peroxidase 1α (P < 0.05) and downregulated the mRNA levels of Kelch-like ECH-associated protein 1 (P > 0.05). The mRNA levels of 4E-binding protein 2 showed the opposite trend compared with those of target of rapamycin, and the minimum value was observed in the group of 1.97% dietary lysine (P < 0.05). In terms of immunity, compared with the 1.44% diet, the 2.44% diet markedly suppressed the intestinal p38 mitogen-activated protein kinase and interferon γ2 mRNA levels (P < 0.05). Moreover, nuclear factor-kappa B p65, tumor necrosis factor α, interleukin 6, interleukin 8, and interleukin 15 mRNA levels all exhibited the same trend as p38 mitogen-activated protein kinase and interferon γ2; however, the difference among all the lysine treatments groups was not significant (P > 0.05). The anti-inflammatory cytokines transforming growth factor β2 and interleukin 4/13B mRNA levels in the intestine were remarkably upregulated by high dietary lysine levels (2.56 and 2.87%) (P < 0.05), and when the dietary lysine level reached 2.44%, the interleukin 4/13A mRNA levels were strikingly increased (P < 0.05). Overall, the data suggested that 2.44% dietary lysine could strengthen the immune and antioxidant capacities of grass carp fry via activating the target of rapamycin (TOR) signaling pathway, and suppressing the p38 mitogen-activated protein kinase (p38 MAPK) signaling pathway, which then improve the survival rate.

show abstract

Section: Discussionsupporting

confidence: 94%

Dietary Lysine Levels Improved Antioxidant Capacity and Immunity via the TOR and p38 MAPK Signaling Pathways in Grass Carp, Ctenopharyngodon idellus Fry

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Earlier studies have shown that the cellular defense capacity is dependent on the activation of the ERK/Nrf2 signaling pathway. Moreover, phosphorylation of ERK is negatively influenced by pro-oxidant exposures [29], while p-ERK is induced by several antioxidant compounds like red ginseng [30], morin [31], curcumin [32] and sulforaphane [33], among others. Treatment of cells with 18α-GA led to increased phosphorylation of ERK whereas treatment with MMC negatively influenced ERK phosphorylation (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Our data suggest that Nrf2 activation by 18α-GA is at least partially regulated by the MAPK (ERK1/2) pathway, as MEK-ERK pathway inhibition by the pharmacological inhibitor PD035901 abolished the protective effects of 18α-GA and reduced the expression of Nrf2 target genes following MMC-induced DNA damage. Many phytochemical components with antioxidant properties have been shown to exert their (usually antioxidant) protective effects through the ERK/Nrf2 pathway; red ginseng [30], morin [31], curcumin [32], sulforaphane [33], genistein [26] and quercetin [37] are only few of them. We have previously demonstrated that in C. elegans 18α-GA promotes the nuclear translocation of SKN-1 (the ortholog of Nrf2 in C. elegans ; [38]) through activation and phosphorylation by PMK-1 (a MAPK family member, ortholog of human MAPK12, 13 and 14) [16].…”

Section: Discussionmentioning

confidence: 99%

The dietary triterpenoid 18α–Glycyrrhetinic acid protects from MMC-induced genotoxicity through the ERK/Nrf2 pathway

et al. 2020

View full text Add to dashboard Cite

18α–Glycyrrhetinic acid (18α-GA) is a bioactive triterpenoid that has been shown to activate the nuclear factor (erythroid-derived-2)-like 2 (Nrf2), the main transcription factor that orchestrates the cellular antioxidant response, in both cellular and organismal context. Although various beneficial properties of 18α-GA have been revealed, including its anti-oxidation and anti-aging activity, its possible protective effect against DNA damage has never been addressed. In this study, we investigated the potential beneficial properties of 18α-GA against DNA damage induced by mitomycin C (MMC) treatment. Using human primary fibroblasts exposed to MMC following pre-treatment with 18α-GA, we reveal an Nrf2-mediated protective effect against MMC-induced cell death that depends on extracellular signal–regulated kinase (ERK) signaling. In total, our results reveal an additional beneficial effect of the Nrf2 activator 18α-GA, suggesting that this important phytochemical compound is a potential candidate in preventive and/or therapeutic schemes against conditions (such as aging) or diseases that are characterized by both oxidative stress and DNA damage.

show abstract

“…Since curcumin has been shown to exert antioxidant effect by inducing several antioxidant defense enzymes in several cell lines [14,23]. Thus, we performed RT-qPCR to detect whether the protective function of curcumin against the oxidative stress induced by H 2 O 2 was associated with antioxidant-associated factors in HTR8/SVneo cells.…”

Section: Curcumin Regulated Mrna Expression Of Multiple Antioxidant Gmentioning

confidence: 99%

Curcumin Protects Human Trophoblast HTR8/SVneo Cells from H2O2-Induced Oxidative Stress by Activating Nrf2 Signaling Pathway

Jiang

Zhang

et al. 2020

Antioxidants

View full text Add to dashboard Cite

Pregnancy complications are associated with oxidative stress induced by accumulation of trophoblastic ROS in the placenta. We employed the human trophoblast HTR8/SVneo cell line to determine the effect of curcumin pre-treatment on H2O2-induced oxidative damage in HTR8/Sveo cells. Cells were pretreated with 2.5 or 5 μM curcumin for 24 h, and then incubated with 400 μM H2O2 for another 24 h. The results showed that H2O2 decreased the cell viability and induced excessive accumulation of reactive oxygen species (ROS) in HTR8/Sveo cells. Curcumin pre-treatment effectively protected HTR8/SVneo cells against oxidative stress-induced apoptosis via increasing Bcl-2/Bax ratio and decreasing the protein expression level of cleaved-caspase 3. Moreover, curcumin pre-treatment alleviated the excessive oxidative stress by enhancing the activity of antioxidative enzymes. The antioxidant effect of curcumin was achieved by activating Nrf2 and its downstream antioxidant proteins. In addition, knockdown of Nrf2 by Nrf2-siRNA transfection abolished the protective effects of curcumin on HTR8/SVneo cells against oxidative damage. Taken together, our results show that curcumin could protect HTR8/SVneo cells from H2O2-induced oxidative stress by activating Nrf2 signaling pathway.

show abstract

Curcumin inhibits heat-induced oxidative stress by activating the MAPK-Nrf2 / ARE signaling pathway in chicken fibroblasts cells

Cited by 47 publications

References 27 publications

Dietary Lysine Levels Improved Antioxidant Capacity and Immunity via the TOR and p38 MAPK Signaling Pathways in Grass Carp, Ctenopharyngodon idellus Fry

Dietary Lysine Levels Improved Antioxidant Capacity and Immunity via the TOR and p38 MAPK Signaling Pathways in Grass Carp, Ctenopharyngodon idellus Fry

The dietary triterpenoid 18α–Glycyrrhetinic acid protects from MMC-induced genotoxicity through the ERK/Nrf2 pathway

Curcumin Protects Human Trophoblast HTR8/SVneo Cells from H2O2-Induced Oxidative Stress by Activating Nrf2 Signaling Pathway

Contact Info

Product

Resources

About