Curcumin Loaded-PLGA Nanoparticles Conjugated with Tet-1 Peptide for Potential Use in Alzheimer's Disease

Abstract

Alzheimer's disease is a growing concern in the modern world. As the currently available medications are not very promising, there is an increased need for the fabrication of newer drugs. Curcumin is a plant derived compound which has potential activities beneficial for the treatment of Alzheimer's disease. Anti-amyloid activity and anti-oxidant activity of curcumin is highly beneficial for the treatment of Alzheimer's disease. The insolubility of curcumin in water restricts its use to a great extend, which can be overcome by the synthesis of curcumin nanoparticles. In our work, we have successfully synthesized water-soluble PLGA coated- curcumin nanoparticles and characterized it using different techniques. As drug targeting to diseases of cerebral origin are difficult due to the stringency of blood-brain barrier, we have coupled the nanoparticle with Tet-1 peptide, which has the affinity to neurons and possess retrograde transportation properties. Our results suggest that curcumin encapsulated-PLGA nanoparticles are able to destroy amyloid aggregates, exhibit anti-oxidative property and are non-cytotoxic. The encapsulation of the curcumin in PLGA does not destroy its inherent properties and so, the PLGA-curcumin nanoparticles can be used as a drug with multiple functions in treating Alzheimer's disease proving it to be a potential therapeutic tool against this dreaded disease.

PLA/PLGA nanoparticles for delivery of drugs across the blood-brain barrier

“…Besides changing the surfactant used in the NP synthesis, the surface of the NP can be modified by the addition of PLGA, 134 nm, oral, DMAB, 400 µg, drug, R [14] PLGA, 177 nm, PVA, i.v., 20 µg, EF, R [17] PLGA, 200 nm, PVA, c.a., 5 mg, EF, R [18] PEG-PLA, 100 nm, none, i.v., 0.6 mg, EF, M [27] PLA, 300 nm, PVA, i.v., 1.125 mg, radioactive drug, M [31] PLGA, 187 nm, F68, i.v., 13.6 µg, EF, R [32] PLGA, 239 nm, n/r, i.v., 82.5 µg, EF, R [36] 25 30 other compounds, such as chitosan or PEG, in an attempt to improve brain NP uptake (Table 2 ). TPGS has been successfully used to prepare NPs, and it was found that TPGS was a more effective and safer emulsifier than PVA, and could improve adsorption of NPs by the gastrointestinal tract [23] .…”

“…P188 was demonstrated to be a better brain targeting agent for PLGA NPs than P80 after intravenous administration in several reported studies [15,17,19,43] . PLA NPs [31] TAT-PLA NPs [31] PLGA NPs [32] g7-PLGA NPs [32] SA-g7-PLGA NPs [32] PLGA NPs [36] TPGS-PLGA NPs [36] Tf-TPGS-PLGA NPs [36] PEG-PLGA NPs [41] Lf-PEG-PLGA NPs [41] Percentage (%) brain uptake of lignad-conjugated nanoparticles…”

“…105 nm, i.v., 60 µg, EF, M [27] 169 nm, i.v., 13.6 µg, EF, R [32] 197 nm, i.v., 13.6 µg, EF, R [32] 340 nm, i.v., 1.13 mg, radioactive drug, M [31] 245 nm, i.v., 82.5 µg, EF, R [36] 120 nm, i. …”

“…Among 29 papers cited in this review, 9 papers performed in vitro studies [20,23,24,31,33,37,38,40] , 8 papers focused on in vivo studies [13,15,16,18,21,25,27,30] , and 12 papers did both in vitro and in vivo studies [14,17,19,22,26,28,29,32,34,35,39,41] .…”

Characterization of Nanoparticles Produced by Chloroform Fraction of Kaempferia Rotunda Rhizome Loaded With Alginic Acid and Chitosan and Its Biological Activity Test

“…The research of curcumin encapsulated that poly lactic-co-glycolic acid (PLGA) nanoparticles can destroy amyloid aggregates, exhibit antioxidative property, and are non-cytotoxic. The encapsulation of the curcumin in PLGA does not destroy its inherent properties and so, the PLGA-curcumin nanoparticles can be used as a drug with multiple functions in treating Alzheimer's disease [24].…”