Curcumin protects BV2 cells against lipopolysaccharide-induced injury via adjusting the miR-362-3p/TLR4 axis

Xie, Ping; Deng, Ming; Sun, Qin-Guo; Jiang, Bo; Hong-jie, XU; Liu, Junqi; Zhou, Yan; Ma, Yonggang; Chen, Zhonghui

doi:10.1007/s11033-020-05543-y

Cited by 11 publications

(5 citation statements)

References 33 publications

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“…Investigation of the nitrite inhibitory effect in LPS-induced BV2 microglia and neuroprotective effects in glutamate-induced HT22 hippocampal cells revealed that intermedin B ( 4 ) and curcumin ( 9 ) exhibited the highest NO inhibitory and neuroprotective effects. However, numerous studies have reported the anti-inflammatory and antioxidant effects of curcumin ( 9 ) [ 45 , 46 , 47 , 48 ]. In this regard, intermedin B ( 4 ) was administered to LPS-induced BV2 microglia to investigate its inhibitory effect on the production of PGE2, an inflammatory cytokine.…”

Section: Resultsmentioning

confidence: 99%

Anti-Neuroinflammatory and Neuroprotective Effect of Intermedin B Isolated from the Curcuma longa L. via NF-κB and ROS Inhibition in BV2 Microglia and HT22 Hippocampal Cells

Lee

Liu

Dong

et al. 2023

IJMS

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Compounds derived from Curcuma longa L. (C. longa) have been extensively studied and reported to be effective and safe for the prevention and treatment of various diseases, but most research has been focused on curcuminoids derived from C. longa. As neurodegenerative diseases are associated with oxidation and inflammation, the present study aimed to isolate and identify active compounds other than curcuminoids from C. longa to develop substances to treat these diseases. Seventeen known compounds, including curcuminoids, were chromatographically isolated from the methanol extracts of C. longa, and their chemical structures were identified using 1D and 2D NMR spectroscopy. Among the isolated compounds, intermedin B exhibited the best antioxidant effect in the hippocampus and anti-inflammatory effect in microglia. Furthermore, intermedin B was confirmed to inhibit the nuclear translocation of NF-κB p-65 and IκBα, exerting anti-inflammatory effects and inhibiting the generation of reactive oxygen species, exerting neuroprotective effects. These results highlight the research value of active components other than curcuminoids in C. longa-derived compounds and suggest that intermedin B may be a promising candidate for the prevention of neurodegenerative diseases.

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Section: Resultsmentioning

confidence: 99%

Anti-Neuroinflammatory and Neuroprotective Effect of Intermedin B Isolated from the Curcuma longa L. via NF-κB and ROS Inhibition in BV2 Microglia and HT22 Hippocampal Cells

Lee

Liu

Dong

et al. 2023

IJMS

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“…MiR-215-5p plays a protective role in the inflammatory injury of septic H9c2 by targeting ILF3 and LRRFIP1 [ 30 ]. Curcumin protects BV2 cells from LPS-induced damage by regulating the miR-362-3p/TLR4 axis [ 31 ]. Among the down-regulated genes, down-regulation of miR-122-5p in Huh7 cells suppressed exosome-induced macrophage activation and macrophage-related inflammation [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Electroacupuncture-driven endogenous circulating serum exosomes as a potential therapeutic strategy for sepsis

Zhang,

Wang,

et al. 2023

Chin Med

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Background Sepsis poses a serious threat to human life and health, with limited options for current clinical treatments. Acupuncture plays an active role in treating sepsis. However, previous studies have focused on the neuromodulatory effect of acupuncture, neglecting its network modulatory effect. Exosomes, as a new way of intercellular communication, may play an important role in transmitting acupuncture information. This paper explores the possibility of electroacupuncture-driven endogenous circulating serum exosomes and their carried miRNAs as a potential treatment for sepsis. Methods The sepsis mouse model was established by intraperitoneal injection of lipopolysaccharide (LPS) (12 mg/kg, 24 mg/kg), and EA (continuous wave, 10 Hz, intensity 5) or intraperitoneal injection of Acupuncture Exosomes (Acu-exo) were performed before the model establishment. The therapeutic effect was evaluated by survival rate, ELISA, H&E staining and lung wet/dry weight ration (W/D). In vivo imaging of small animals was used to observe the accumulation of Acu-exo in various organs of sepsis mice. LPS was used to induce macrophages in cell experiments, and the effect of Acu-exo on macrophage inflammatory cytokines was observed. In addition, The miRNA sequencing method was further used to detect the serum exosomes of normal and EA-treated mice, and combined with network biology analysis methods to screen possible key targets. Results EA and Acu-exo reduced the W/D and lung tissue damage in sepsis mice, down-regulated the expression of serum inflammatory cytokines TNF-α and IL-6, and increased the survival rate of sepsis mice. In vivo imaging of small animals found that Acu-exo were accumulated in the lungs of sepsis mice. Cell experiments proved that Acu-exo down-regulated the expression of inflammatory cytokines TNF-α, IL-6 and IL-1β to alleviate the inflammatory response induced by LPS in macrophages. MiRNA sequencing revealed 53 differentially expressed miRNAs, and network biology analysis revealed the key targets of Acu-exo in sepsis treatment. Conclusion Electroacupuncture-driven endogenous circulating serum exosomes and their carried miRNAs may be a potential treatment for sepsis.

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“…MiRNAs in this panel have known roles in cancer progression ( Che et al., 2019 ; Hsu et al., 2016 ; Huang et al., 2018 ; Kobayashi et al., 2012 ; Kohram et al., 2018 ; Li et al., 2011 , 2016 ; Ling et al., 2019 ; Lv et al., 2018 ; Mogilyansky and Rigoutsos, 2013 ), inflammation and inflammatory diseases ( Paraskevi et al., 2012 ; Reddycherla et al., 2015 ; Wu et al., 2011 ; Zheng et al., 2015 ), and neurodegeneration ( Basak et al, 2016 ; Denk et al., 2015 ; Jee et al., 2012b ; Khoo et al., 2012 ; Liu et al., 2017 ). These miRNAs have also been shown to target genes that have neuroinflammatory functions and roles in diseases with neuroinflammatory pathophysiology ( Cao et al., 2017 ; Lisha Chang et al., 2020 ; Gao et al., 2019 ; Gayen et al., 2020 ; Hu et al., 2019 ; Jee et al., 2012a ; Jiang et al., 2018 ; Ma et al., 2014 ; Mao et al., 2019 ; Martinez and Peplow, 2017 ; Naqvi et al., 2016 ; Navaderi et al., 2019 ; Peng and Ying, 2014 ; Shi et al., 2018 ; Sinha et al., 2012 ; Sujith et al., 2018 ; Thangaraj et al., 2021 ; Wang et al., 2021 ; Xie et al., 2020 ; Yang et al., 2018 ; Yao et al., 2014 ; Zhao et al., 2017 ; Zhong et al., 2021 ; Zhou et al., 2021 ). Post-hoc miRNA-gene network analyses further solidified the involvement of these nine miRNAs in mitochondrial processes and neuroinflammation (see Tables S6–S8 , Figures S6–S8 ).…”

Section: Discussionmentioning

confidence: 99%

A preliminary model of football-related neural stress that integrates metabolomics with transcriptomics and virtual reality

et al. 2022

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Research suggests contact sports affect neurological health. This study used permutation-based mediation statistics to integrate measures of metabolomics, neuroinflammatory miRNAs, and virtual reality (VR)-based motor control to investigate multi-scale relationships across a season of collegiate American football. Fourteen significant mediations (six pre-season, eight across-season) were observed where metabolites always mediated the statistical relationship between miRNAs and VR-based motor control (p perm Sobel % 0.05; total effect > 50%), suggesting a hypothesis that metabolites sit in the statistical pathway between transcriptome and behavior. Three results further supported a model of chronic neuroinflammation, consistent with mitochondrial dysfunction: (1) Mediating metabolites were consistently medium-to-long chain fatty acids, (2) tricarboxylic acid cycle metabolites decreased across-season, and (3) accumulated head acceleration events statistically moderated pre-season metabolite levels to directionally model post-season metabolite levels. These preliminary findings implicate potential mitochondrial dysfunction and highlight probable peripheral blood biomarkers underlying repetitive head impacts in otherwise healthy collegiate football athletes.

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Curcumin protects BV2 cells against lipopolysaccharide-induced injury via adjusting the miR-362-3p/TLR4 axis

Cited by 11 publications

References 33 publications

Anti-Neuroinflammatory and Neuroprotective Effect of Intermedin B Isolated from the Curcuma longa L. via NF-κB and ROS Inhibition in BV2 Microglia and HT22 Hippocampal Cells

Anti-Neuroinflammatory and Neuroprotective Effect of Intermedin B Isolated from the Curcuma longa L. via NF-κB and ROS Inhibition in BV2 Microglia and HT22 Hippocampal Cells

Electroacupuncture-driven endogenous circulating serum exosomes as a potential therapeutic strategy for sepsis

A preliminary model of football-related neural stress that integrates metabolomics with transcriptomics and virtual reality

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