Curcumin regulates gene expression of insulin like growth factor, B-cell CLL/lymphoma 2 and antioxidant enzymes in streptozotocin induced diabetic rats

Abstract

BackgroundThe effects of curcumin on the activities and gene expression of antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione-S-transferase (G-ST), B-cell CLL/lymphoma 2 (Bcl-2) and insulin like growth factor-1 (IGF-1) in diabetic rats were studied.MethodsTwenty four rats were assigned to three groups (8 rats for each). Rats of first group were non diabetic and rats of the second group were rendered diabetic by streptozotocin (STZ). Both groups received vehicle, corn oil only (5 ml/kg body weight) and served as negative and positive controls, respectively. Rats of the third group were rendered diabetic and received oral curcumin dissolved in corn oil at a dose of 15 mg/5 ml/kg body weight for 6 weeks.ResultsDiabetic rats showed significant increase of blood glucose, thiobarbituric acid reactive substances (TBARS) and activities of all antioxidant enzymes with significant reduction of reduced glutathione (GSH) compare to the control non diabetic group. Gene expression of Bcl2, SOD, CAT, GPX and GST was increased significantly in diabetic untreated rats compare to the control non diabetic group. The administration of curcumin to diabetic rats normalized significantly their blood sugar level and TBARS values and increased the activities of all antioxidant enzymes and GSH concentration. In addition, curcumin treated rats showed significant increase in gene expression of IGF-1, Bcl2, SOD and GST compare to non diabetic and diabetic untreated rats.ConclusionCurcumin was antidiabetic therapy, induced hypoglycemia by up-regulation of IGF-1 gene and ameliorate the diabetes induced oxidative stress via increasing the availability of GSH, increasing the activities and gene expression of antioxidant enzymes and Bcl2. Further studies are required to investigate the actual mechanism of action of curcumin regarding the up regulation of gene expression of examined parameters.

Curcumin Stimulates Biochemical Mechanisms of Apis Mellifera Resistance and Extends the Apian Life-Span

“…In addition, GSH conjugates with xenobiotic substances with the aid of GST (El-Bahr, 2013;Trujillo et al, 2013). Consequently, our results show that curcumin not only improves the antioxidative potential in mammals (Trujillo et al, 2013), but also, and even particularly, in bees.…”

Effect of Curcumin on Hepatic Antioxidant Enzymes Activities and Gene Expressions in Rats Intoxicated with Aflatoxin B1

“…It has been proposed that the toxic effect of AFB 1 may be attributed to an elevated level of reactive oxygen species (ROS) produced during its metabolism in the liver (Towner et al , ). Medicinal plants and their active principles have received great interest as potentially antiperoxidative agents (El‐Bahr, ; El‐Bahr, ). Curcumin, the active ingredient of the spice turmeric, is a potent agent with antioxidant (El‐Bahr, ; Sahebkar et al , ), anti‐inflammatory (Chainani‐Wu, ; Panahi et al , ), anti‐diabetic (El‐Bahr, ; Neerati et al , ) and anti‐cancer (Belcaro et al , ; Thongrakard et al , ; Yamauchi et al , ).…”

“…Medicinal plants and their active principles have received great interest as potentially antiperoxidative agents (El‐Bahr, ; El‐Bahr, ). Curcumin, the active ingredient of the spice turmeric, is a potent agent with antioxidant (El‐Bahr, ; Sahebkar et al , ), anti‐inflammatory (Chainani‐Wu, ; Panahi et al , ), anti‐diabetic (El‐Bahr, ; Neerati et al , ) and anti‐cancer (Belcaro et al , ; Thongrakard et al , ; Yamauchi et al , ). Besides the antiviral effect, the ameliorative effect of curcumin against biliary disorders, anorexia, cough, rheumatism and sinusitis has been also documented (Gupta et al , ).…”

Mechanism of Tang Luo Ning effect on attenuating of oxidative stress in sciatic nerve of STZ-induced diabetic rats

“…Oxidative stress has been shown to regulate apoptosis (El-Bahr, 2013). Bcl2 is critical for regulation of apoptosis across diverse cell types, and it is activated in response oxidative stress (Frenzel et al, 2009).…”