Effect of Curcumin on Hepatic Antioxidant Enzymes Activities and Gene Expressions in Rats Intoxicated with Aflatoxin B1

El-Bahr, Sabry M.

doi:10.1002/ptr.5239

Cited by 114 publications

(98 citation statements)

References 33 publications

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“…According to this author, the deficiency in CAT activity results in oxidative stress, contributing to disease pathogenesis, due to increased levels of free radical hydrogen peroxides. In addition, the same significative reduction in CAT activity has been reported in the liver of rats aflatoxin-fed [10].…”

Section: Discussionsupporting

confidence: 79%

The Protective Effects of an Adsorbent against Oxidative Stress in Quails Fed Aflatoxin-Contaminated Diet

Migliorini

Silva

Santúrio

et al. 2017

Acta Scientiae. Vet.

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Background: Contamination of crops with aflatoxin is considered a serious global threat to food safety, since potent carcinogenic, teratogenic, mutagenic and immunosuppressive effects of aflatoxins are well recognized. Recently, the use of adsorbents has been linked with protective effects against oxidative stress in several diseases. Thus, the aim of this study was to assess the occurrence of oxidative stress in quails (Coturnix coturnix) fed with aflatoxin-contaminated diet, as well as the protective effect of an adsorbent. Materials, Methods & Results: Twenty-eight quails were divided into four groups (n = 7): diet without additives (control; the group A), diet and adsorbent containing aluminosilicates (the group B), aflatoxin-contaminated diet (200 ppb) (the group C), and aflatoxin-contaminated diet (200 ppb) and adsorbent containing aluminosilicates (the group D). The composition of the adsorbent containing aluminosilicates was 0.3% based on yeast cell wall, silymarin, and bentonite. The animals received feed and water ad libitum during 20 days. At the end of the experimental period, total blood was collected by cardiac puncture in tubes without anticoagulant to obtain serum (centrifuged at 3500 g during 10 min) for later determination of biochemical parameters. The liver was placed in a solution of Tris-HCl 10 mM, pH 7.4 for TBARS (Thiobarbituric acid reactive substances), ROS (Reactive oxygen species), SOD (Superoxide dismutase) and CAT (Catalase) analysis. The hepatic tissue was gently homogenized in a glass potter in specific buffer, homogenated, and centrifuged at 10.000 g at 4ºC for 10 min to yield a supernatant (S1) used for analyses. Homogenate aliquots were stored at -80°C until utilization. Fragments of liver and intestine (5 cm) were collected for histopathological analyses. Between days 15 to 20 of the experiment, group C quails showed clinical signs, such as apathy, creepy feathers and reduced feed intake. At day 20 of experiment, macroscopically, the liver of quails belonging to the group C showed greenish yellow color differently from the other groups. Microscopically, no alterations were observed in the liver of animals in groups A and B. Severe diffuse microvacuolar degeneration (hydropic) of hepatocytes and small foci of necrosis in the liver were observed in the group C, as observed in the group D, but in a more moderate degree to microvacuolar degeneration. Seric total protein, albumin, globulin and uric acid levels decreased in the group C and D. The levels of alanine aminotransferase (ALT) increased in the group C, and the treatment with adsorbent was able to avoid this increment. Seric and hepatic reactive oxygen species and TBARS increased in the group C, and the treatment with adsorbent reduced theses parameters in the group D. Catalase (CAT) activity decreased, while ALA-D increased in the group C. The treatment with adsorbent was able to prevent CAT activity decrease, but it did not prevent the increase in ALA-D activity. Discussion: Aflatoxins are considered one of the mo...

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Section: Discussionsupporting

confidence: 79%

The Protective Effects of an Adsorbent against Oxidative Stress in Quails Fed Aflatoxin-Contaminated Diet

Migliorini

Silva

Santúrio

et al. 2017

Acta Scientiae. Vet.

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“…It possesses antioxidant, anti-inflammatory, radio-protective, chemotherapeutic, anti-cancer, and detoxification abilities in laboratory animals and humans [14,15,16,17]. Previous publications have described that CM can effectively mitigate AFB 1 -induced adverse effects in several animal species [6,15,18,19,20]. Moreover, the protective action of CM against AFB 1 -induecd adverse effects was further demonstrated by improving the antioxidant capacity [18,19,21,22].…”

Section: Introductionmentioning

confidence: 99%

Curcumin Prevents Aflatoxin B1 Hepatoxicity by Inhibition of Cytochrome P450 Isozymes in Chick Liver

Zhang

Zhao

et al. 2016

Toxins

113

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This study was designed to establish if Curcumin (CM) alleviates Aflatoxin B1 (AFB1)-induced hepatotoxic effects and to determine whether alteration of the expression of cytochrome P450 (CYP450) isozymes is involved in the regulation of these effects in chick liver. One-day-old male broilers (n = 120) were divided into four groups and used in a two by two factorial trial in which the main factors included supplementing AFB1 (< 5 vs. 100 μg/kg) and CM (0 vs. 150 mg/kg) in a corn/soybean-based diet. Administration of AFB1 induced liver injury, significantly decreasing albumin and total protein concentrations and increasing alanine aminotransferase and aspartate aminotransferase activities in serum, and induced hepatic histological lesions at week 2. AFB1 also significantly decreased hepatic glutathione peroxidase, catalase, and glutathione levels, while increasing malondialdehyde, 8-hydroxydeoxyguanosine, and exo-AFB1-8,9-epoxide (AFBO)-DNA concentrations. In addition, the mRNA and/or activity of enzymes responsible for the bioactivation of AFB1 into AFBO—including CYP1A1, CYP1A2, CYP2A6, and CYP3A4—were significantly induced in liver microsomes after 2-week exposure to AFB1. These alterations induced by AFB1 were prevented by CM supplementation. Conclusively, dietary CM protected chicks from AFB1-induced liver injury, potentially through the synergistic actions of increased antioxidant capacities and inhibition of the pivotal CYP450 isozyme-mediated activation of AFB1 to toxic AFBO.

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“…Curcumin is traditionally known having some effects totreat some diseases in human [23]. Curcumin is able to up-regulation of antioxidant enzyme gene expression, activation of the expressed genes and increase in the availability of glutathione (GSH) [24][25]. Curcuminoid is capable to scavenging the radical acetyl hydrazine and hydrazine which is generated by metabolism of isoniazid [26].…”

Section: Discussionmentioning

confidence: 99%

Hepatoprotective Activity Combination of Curanga Fel-Terrae Lour Leaves and Curcuma Heyneana Valeton and Zijp Rhizome in Rat Induced by Combination of Rifampin and Isoniazid

Marianne

Chrestella

Ginting

et al. 2017

Int J Pharm Pharm Sci

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Objective: The liver is a vital organ in the body, it is often exposured to the xenobiotics that can cause injury. Pugun tano (Curanga fel-terrae) and temu giring (Curcuma heyneana) are plants that have been claimed to cure many ailments including protecting the liver. But the hepatoprotective properties of the combination of these plants has not been well studied. In this regard, the current study was undertaken to evaluate the activity combination of those plants. Methods:The male Wistar rats were divided into 6 groups, group I was a negative control (CMC-Na); group II-IV were the treatment groups and were given combination extracts at the doses of 50 mg/kg, 75 mg/kg and 100 mg/kg respectively; group V was positive control (catechin) and group VI was normal control. All of groups except group VI were given combination rifampin 100 mg/kg and isoniazid 50 mg/kg for 15 d along with administration of extracts. At the day 16, rats were sacrificed. Histopathology of the liver and biochemical assay of blood was done at the end of the administration. Results:The administration of the combination of C. fel-terrae and C. heyneana at the doses of 75 and 100 mg/kg significantly inhibited the elevation of aspartate aminotransferase (AST) and alanine transaminase (ALT) compared to negative control (p<0.05). Histopathological assessment of the liver was comparable to the normal control (p>0.05). Conclusion:The combination of C. fel-terrae and C. heyneana at the dose of 75 and 100 mg/kg is able to protect the liver from damage induced by rifampin and isoniazid.

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Effect of Curcumin on Hepatic Antioxidant Enzymes Activities and Gene Expressions in Rats Intoxicated with Aflatoxin B1

Cited by 114 publications

References 33 publications

The Protective Effects of an Adsorbent against Oxidative Stress in Quails Fed Aflatoxin-Contaminated Diet

The Protective Effects of an Adsorbent against Oxidative Stress in Quails Fed Aflatoxin-Contaminated Diet

Curcumin Prevents Aflatoxin B1 Hepatoxicity by Inhibition of Cytochrome P450 Isozymes in Chick Liver

Hepatoprotective Activity Combination of Curanga Fel-Terrae Lour Leaves and Curcuma Heyneana Valeton and Zijp Rhizome in Rat Induced by Combination of Rifampin and Isoniazid

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