extract has been investigated for its hepatoprotective activity. This current study was conducted to evaluate the subchronic toxic effect of Lour. leaf extract. The subchronic toxicity of ethanol extract of leaf was evaluated by OECD (Organization for Economic Cooperation and Development) guidelines. The extract at doses of 125, 250, 500, 1000 mg/kg body weight (BW) were orally administered to the test animals for 90 days. Signs of toxicity and mortality were observed for 90 days and 118 days. The biochemistry, hematology, macropathology, relative organ weight and histopathology examination was also performed. The macroscopic and microscopic examinations of liver, lung, kidney, spleen and heart displayed normal color and texture after treatment, with ethanol extract leaf at doses of 125 and 250 mg/kg BW. The signs of toxicity, mortality, macroscopic and microscopic changes, as well as increasing biochemistry parameters were observed after treatment, with extract at the dose of 500 and 1000 mg/kg BW. However, the abnormal value of biochemistry parameters was returned to normal when the treatment was stopped. There was no effect on hematological parameters at all doses of extract. The ethanol extract leaf is untoxic for long-term use at dose 125 and 250 mg/kg BW. The toxic effect of at 500 and 1000 mg/kg BW was reversible.

The making of perfume from the combination Magnolia  alba (M. alba) flower oil, Cananga odorata (C. odorata) and Mimusops elengi L (M. elengi) fixed with Pogostemon cablin oil (P. cablin) have been done. Essential oil of M. alba and C. odorata was distilled by water distillation and extraction essential oil of M. elengi flower using n-hexane evaporated solvent method. Composition perfume through organoleptic test obtained with a ratio of 4% v/v, 4% v/v, and 2% v/v for each essential oil M. alba, C. odorata and M. elengi. Antibacterial activity of the perfume and each of the volatile oil was performed by the disc diffusion method. Antibacterial activity test showed that perfumes were able to inhibit the growth of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) bacteria, but less active. Antibacterial activity of the perfume was more active against E. coli with an inhibitory zone of 8.3 mm and 39.81% inhibitory power  than S. aureus bacterial 7.3 mm inhibition zone with 37.55% inhibitory power. Perfumes after storage for  one, and two months had better antibacterial activity than before storage with an average inhibitory zone of 11-15 mm and inhibitory power of 50- 68,18%.   Keywords: Antibacterial, E. Coli, C. Odorata, M. alba, M. elengi, P. cablin, , S. aureus

To investigate the link between adolescents' experiences of participating in a weight-loss programme and their weight status, Health-Related Quality of Life and selfconcept over 5 years. Design and Method: A qualitative study with a hermeneutic approach, focused on interpreting adolescents' experiences. Interviews of 10 adolescents in a weight-loss programme, and their parents, three times over 5 years. The interview findings were supported by BMI-SDS (Body Mass Index standard deviation scores) and questionnaires: Health-Related Quality of Life (HRQOL), and self-concept (Beck Youth Inventories, BSCI-Y). Results: Immediately after completion of the weight-loss programme, the 10 adolescents had all lost weight and experienced other effects, e.g. more energy, making friends and discovering commonalities, along with improved physical parameters, experienced HRQOL and self-concept. However, weight loss was difficult to maintain after completion. Some adolescents who had regained weight 1 year after completion reported lower perceived HRQOL and self-concept and also left the study. Conclusion: Weight loss can be achieved during a treatment programme for overweight adolescents, bringing lower BMI and also potential psycho-social benefits. Focus on weight loss alone may involve overlooking other positive sideeffects of the treatment. There are also overlooked negative effects: Not all participants maintained weight loss, and for regainers, some HRQOL and self-concept scores deteriorated, compared to their situation pre-programme. These negative effects are not described in other studies. Practice Implications: We encourage enhanced professional support for drop-outs and regainers, and we recommend ongoing, open-ended programmes to better retain and support adolescents and their families. Our findings indicate this would improve intervention outcomes for the entire group.

The in vivo transformation of cannabinol (CBN) in the rat has been studied. Unchanged CBN and nine neutral mono‐oxygenated and dioxygenated CBN metabolites have been identified. In the mono‐oxygenated series the metabolites occurred in decreasing order of prominence as follows: 7‐hydroxy‐CBN, 4“‐hydroxy‐CBN, 1”‐hydroxy‐CBN, 2“‐hydroxy‐CBN, 3”‐hydroxy‐CBN, 5“‐hydroxy‐CBN and CBN‐7‐al. In the dihydroxylated metabolite series only 1”,7‐dihydroxy‐CBN and 4“,7‐dihydroxy‐CBN were found with the former as the more prominent metabolite.

The extract n-hexane and ethyl acetate obtained from plants Ficus racemosa, respectively of 68.50 g (2.28%) and 50