Curcumin Suppresses Malignant Glioma Cells Growth and Induces Apoptosis by Inhibition of <scp>SHH</scp>/<scp>GLI</scp>1 Signaling Pathway <i>in Vitro</i> and <i>Vivo</i>

Du, Wei; Yan, Feng; Piao, Xing-Yin; Cui, Yuqiong; Chen, Lingchao; Lei, Xuhui; Sun, Xu; Liu, Xing; Wang, Hanbing; Li, Xianfeng; Yang, Dan; Sun, Ying; Zhao, Zhenqun; Jiang, Tao; Li, Yongli; Jiang, Chuanlu

doi:10.1111/cns.12163

Cited by 80 publications

(64 citation statements)

References 40 publications

(50 reference statements)

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“…[46][47][48] Previous studies found 49,50 that the inhibitory mechanism of p53 that relied on NF-κB existed in the kidney cancer cells. Quinacrine inhibited NF-κB and activated tumor suppressors of p53 simultaneously.…”

Section: Discussionmentioning

confidence: 99%

The functional curcumin liposomes induce apoptosis in C6 glioblastoma cells and C6 glioblastoma stem cells in vitro and in animals

Wang

Xue

et al. 2017

IJN

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Glioblastoma is a kind of malignant gliomas that is almost impossible to cure due to the poor drug transportation across the blood–brain barrier and the existence of glioma stem cells. We prepared a new kind of targeted liposomes in order to improve the drug delivery system onto the glioma cells and induce the apoptosis of glioma stem cells afterward. In this experiment, curcumin was chosen to kill gliomas, while quinacrine was used to induce apoptosis of the glioma stem cells. Also, p -aminophenyl-α-D-mannopyranoside could facilitate the transport of liposomes across the blood–brain barrier and finally target the brain glioma cells. The cell experiments in vitro indicated that the targeted liposomes could significantly improve the anti-tumor effects of the drugs, while enhancing the uptake effects, apoptosis effects, and endocytic effects of C6 glioma cells and C6 glioma stem cells. Given the animal experiments in vivo, we discovered that the targeted liposomes could obviously increase the survival period of brain glioma-bearing mice and inhibit the growth of gliomas. In summary, curcumin and quinacrine liposomes modified with p -aminophenyl-α-D-mannopyranoside is a potential preparation to treat brain glioma cells and brain glioma stem cells.

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Section: Discussionmentioning

confidence: 99%

The functional curcumin liposomes induce apoptosis in C6 glioblastoma cells and C6 glioblastoma stem cells in vitro and in animals

Wang

Xue

et al. 2017

IJN

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“…13,17 Briefly, the tumor tissues were deparaffinaged, rehydrated, treated with 0.3% hydrogen peroxide, and processed with antigen retrieval using heat induction for about 10 minutes. Samples were stained with anti-E-cadherin antibody (1:200, Proteintech) and anti-Vimentin antibody (1:200, Cell Signaling Technology).…”

Section: Immunohistochemistry (Ihc) Assaymentioning

confidence: 99%

“…13 Then we used Western Blot to test the EMT markers and the Hedgehog signaling pathway, which was reported to be the target signaling pathway of Curcumin. We chose 96 hours as a time period after g-irradiation.…”

Section: Introductionmentioning

confidence: 99%

Curcumin increases efficiency of γ-irradiation in gliomas by inhibiting Hedgehog signaling pathway

et al. 2017

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It was reported that g-irradiation had a controversial therapeutic effect on glioma cells. We aimed to investigate the cytotoxic effect on the glioma cells induced by g-irradiation and explore the treatment to rescue the phenotype alteration of remaining cells. We used transwell assay to detect the glioma cell invasion and migration capacity. Cell proliferation and apoptosis were tested by the CCK-8 assay and flow cytometry respectively. Western Blot was used to detect the activity of Hedgehog signaling pathway and Epithelial-to-Mesenchymal Transition (EMT) status. g-irradiation showed cytotoxic effect on LN229 cells in vitro, whereas this contribution was limited in U251 cells. However, it could significantly stimulated EMT process in both LN229 and U251. Curcumin (CCM) could rescue EMT process induced by g-irradiation via the suppression of Gli1 and the upregulation of Sufu. The location and expression of EMT markers were also verified by Immunofluorescence. Immunohistochemistry assay was used on intracranial glioma tissues of nude mice. The capacities of cell migration and invasion were suppressed with combined therapy. This research showed Curcumin could rescue the EMT process induced by g-irradiation via inhibiting the Hedgehog signaling pathway and potentiate the cell cytotoxic effect in vivo and in vitro.

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“…Various concentrations of β-elemene (Dalian Jingang Pharmaceutical Co. China) were used for 20h.Then 5 mg/ml MTT (Sigma-Aldrich, USA) was added to each well for another 4h as reported previously (Du WZ et al, 2013). The amount of formazan was assessed by measuring the absorbance using TECAN microplate reader at 490nm.…”

Section: Cytotoxicity Assaymentioning

confidence: 99%

“…Cells were cultured for 12 days with complete medium. Then the colonies obtained were washed with PBS and fix in 10% formalin for 10min at room temperature (Du et al, 2013). Samples were stained with Giemsa stain and the number of colonies with more than 50 cells was counted.…”

Section: Cell Proliferation Assay and Colony Formation Assaymentioning

confidence: 99%

β-elemene Induces Caspase-dependent Apoptosis in Human Glioma Cells in vitro through the Upregulation of Bax and Fas/FasL and Downregulation of Bcl-2

Chang²,

Guo³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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Curcumin Suppresses Malignant Glioma Cells Growth and Induces Apoptosis by Inhibition of SHH/GLI1 Signaling Pathway in Vitro and Vivo

Abstract: This study shows that curcumin holds a great promise for SHH/GLI1 targeted therapy against gliomas.

Cited by 80 publications

References 40 publications

The functional curcumin liposomes induce apoptosis in C6 glioblastoma cells and C6 glioblastoma stem cells in vitro and in animals

The functional curcumin liposomes induce apoptosis in C6 glioblastoma cells and C6 glioblastoma stem cells in vitro and in animals

Curcumin increases efficiency of γ-irradiation in gliomas by inhibiting Hedgehog signaling pathway

β-elemene Induces Caspase-dependent Apoptosis in Human Glioma Cells in vitro through the Upregulation of Bax and Fas/FasL and Downregulation of Bcl-2

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