Curcuminoid content of Curcuma longa L. and Curcuma xanthorrhiza rhizome based on drying method with NMR and HPLC-UVD

Hadi, Syamsul; Artanti, Anif Nur; Rinanto, Yudi; Wahyuni, Dinar Sari Cahyaningrum

doi:10.1088/1757-899x/349/1/012058

Cited by 20 publications

(15 citation statements)

References 7 publications

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“…The HPLC analysis conditions were: Jasco PU-2089 Plus liquid chromatography equipped with a Rheodyne 7725i injector (fitted with a 20 μL loop), a Jasco UV-2075 HPLC detector operating at 420 nm, Jasco-Borwin integrator (Jasco Europe s.r.l., Milan, Italy), and a Tracer Excel 120 ODS-A column particle size 5 μm, 15 × 0.4 cm (Barcelona, Spain), mobile phase consisting of methanol at a flow rate of 1.0 mL min −1 [37].…”

Section: Methodsmentioning

confidence: 99%

Graphene Oxide Functional Nanohybrids with Magnetic Nanoparticles for Improved Vectorization of Doxorubicin to Neuroblastoma Cells

Lerra

Farfalla

Sanz³

et al. 2018

Pharmaceutics

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With the aim to obtain a site-specific doxorubicin (DOX) delivery in neuroblastoma SH-SY5Y cells, we designed an hybrid nanocarrier combining graphene oxide (GO) and magnetic iron oxide nanoparticles (MNPs), acting as core elements, and a curcumin–human serum albumin conjugate as functional coating. The nanohybrid, synthesized by redox reaction between the MNPs@GO system and albumin bioconjugate, consisted of MNPs@GO nanosheets homogeneously coated by the bioconjugate as verified by SEM investigations. Drug release experiments showed a pH-responsive behavior with higher release amounts in acidic (45% at pH 5.0) vs. neutral (28% at pH 7.4) environments. Cell internalization studies proved the presence of nanohybrid inside SH-SY5Y cytoplasm. The improved efficacy obtained in viability assays is given by the synergy of functional coating and MNPs constituting the nanohybrids: while curcumin moieties were able to keep low DOX cytotoxicity levels (at concentrations of 0.44–0.88 µM), the presence of MNPs allowed remote actuation on the nanohybrid by a magnetic field, increasing the dose delivered at the target site.

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Section: Methodsmentioning

confidence: 99%

Graphene Oxide Functional Nanohybrids with Magnetic Nanoparticles for Improved Vectorization of Doxorubicin to Neuroblastoma Cells

Lerra

Farfalla

Sanz³

et al. 2018

Pharmaceutics

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“…(Baharun et al 2013). Selain itu, senyawa curcumin dan demethoxycurcumin seperti yang diperoleh dalam penelitian ini lebih sering dimanfaatkan sebagai antioksidan, antiinflamasi, antibakteri, dan antikarsinogenik pada manusia (Hadi et al 2018). Hasil penelitian ini menunjukkan bahwa senyawa nonvolatil ekstrak rimpang C. aeruginosa mampu menghambat perkembangan penyakit antraknosa pada cabai merah dengan tingkat penghambatan mencapai 100% (Diagram 1) sehingga mampu menjadi alternatif pengendalian fitopatogen yang potensial untuk dikembangkan khususnya pada tanaman hortikultura.…”

Section: Hasil Dan Pembahasanunclassified

Efektivitas Antifungi Ekstrak Curcuma aeruginosa terhadap Patogenisitas Colletotrichum capsici pada Tanaman Cabai Merah (Antifungi Effectivity of Curcuma aeruginosa Extract to Colletotrichum capsici Pathogenicity on Red Chilli Pepper)

Sari

Li’aini

2021

J.Hort

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Antraknosa yang disebabkan oleh Colletotrichum capsici masih menjadi penyakit utama yang menyerang buah cabai merah. Selama ini, penggunaan Curcuma aeruginosa sebagai antimikrobe patogen penyebab penyakit pada manusia lebih populer daripada sebagai antimikrobe fitopatogen. Rimpang Curcuma diketahui mengandung senyawa volatil/atsiri dan nonvolatil. Potensi senyawa volatil/atsiri Curcuma sebagai antimikrobe telah banyak dilaporkan, namun masih sangat terbatas untuk senyawa nonvolatilnya. Penelitian ini bertujuan mengetahui efektivitas antifungi senyawa nonvolatil dari ekstrak C. aeruginosa terhadap C. capsici pada buah cabai merah. Penelitian dilaksanakan pada bulan November 2014 hingga Mei 2015 di Universitas Brawijaya. Senyawa nonvolatil didapatkan dengan merendam rimpang C. aeruginosa menggunakan pelarut metanol kemudian didistilasi menggunakan rotary vacuum evaporator dan diidentifikasi menggunakan HPLC. Efektivitas antifungi senyawa nonvolatil dari ekstrak rimpang C. aeruginosa diuji secara in vitro dan in vivo di laboratorium menggunakan Rancangan Acak Lengkap dengan tiga kali ulangan. Perlakuan yang diujikan yaitu konsentrasi senyawa nonvolatil terdiri atas 0 (kontrol), 4, 6, 8, 10, dan 12 ppm. Senyawa nonvolatil dari ekstrak rimpang C. aeruginosa sangat efektif menghambat pertumbuhan C. capsici baik secara in vivo maupun in vitro di laboratorium bahkan konsentrasi 12 ppm menunjukkan persentase penghambatan antraknosa hingga 100%. Hasil identifikasi HPLC menunjukkan bahwa senyawa nonvolatil dari ekstrak rimpang C. aeruginosa mengandung kelompok Curcuminoid yang terdiri atas curcumin dan demethoxycurcumin yang berperan sebagai antifungi sehingga sangat berpotensi dikembangkan sebagai fungisida nabati.KeywordsAntraknosa; Cabai merah; C. aeruginosa; Senyawa nonvolatilAbstractAnthracnose caused by Colletotrichum capsici is still as major disease against chilli pepper fruit. During this time, utilization of C. aeruginosa as pathogen antimicrobial caused disease to human is more popular than to crops. Curcuma has been known containing volatile and nonvolatile compound. Potential of volatil compound from Curcuma as antipathogen has been reported widely, nevertheless it is still limited known for nonvolatile compound. This research aimed to understand the antifungi effectivity of nonvolatile compound from C. aeruginosa extract to C. capsici on chilli pepper fruit. Research was conducted in November 2014 to Mei 2015. Nonvolatile compound was obtained by soaking C. aeruginosa rhizome into methanol solvent then distilated using rotary vacuum evaporator and identified with HPLC instrument. Antifungi effectivity of nonvolatile compound from C. aeruginosa extract was experimented by in vitro and in vivo test using Completely Randomized Design with three replications. Treatments tested were various concentrations of nonvolatile compound namely 0 (control), 4, 6, 8,10, and 12 ppm. Nonvolatile compound from C. aeruginosa extract was highly effective to inhibit growth of C. capsici by both in vitro and in vivo test. HPLC identification result showed nonvolatile compound from C. aeruginosa extract contains Curcuminoid group play role as antifungi.

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“…The second significant group of C. longa L. compounds are curcuminoids, which include mainly 3 components: curcumin, desmethoxycurcumin and bisdemethoxycurcumin [8,9,10]. The structure, composition and quantitative evaluation of curcuminoids in roots and various plant compositions containing curcuminoids was carried out by different methods, namely nuclear magnetic resonance spectroscopy, high-performance liquid chromatography with ultraviolet and mass detection, circular chromatography [11][12][13][14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Prospects of Using Freons as Extractants of Curcuma Longa L. Root Essential Oil

Zhilyakova¹,

Новиков²,

Писарев³

et al. 2019

Proceedings of the 1st International Symposium Innovations in Life Sciences (ISILS 2019)

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the purpose of the present study was a comparative study of the ability of some freons to extract the essential oil of the roots of C. longa L. and to provide optimal conditions for its analysis. The dried, crushed roots of C. longa L. were used as raw materials (country of origin -Uganda). Methoxynonafluorobutane (Novec 7100), fluoroketone and nhexane were used as extractants. Method of gas-liquid chromatography with mass detection was used. The most efficient extractant for ar-tumerone and curlone, which proved to be methoxynonafluorobutane (Novec 7100). The results of the studies indicate the prospects of using freons as extracting agents for the extraction of essential oil from the roots of C. longa L. The prospect consists in a more effective extracting ability of organofluorine solvents than n-hexane to increase the yield of both, the essential oil itself and the most pharmacologically relevant root components of C. longa L.ar-tumerone and curlone.

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Curcuminoid content of Curcuma longa L. and Curcuma xanthorrhiza rhizome based on drying method with NMR and HPLC-UVD

Cited by 20 publications

References 7 publications

Graphene Oxide Functional Nanohybrids with Magnetic Nanoparticles for Improved Vectorization of Doxorubicin to Neuroblastoma Cells

Graphene Oxide Functional Nanohybrids with Magnetic Nanoparticles for Improved Vectorization of Doxorubicin to Neuroblastoma Cells

Efektivitas Antifungi Ekstrak Curcuma aeruginosa terhadap Patogenisitas Colletotrichum capsici pada Tanaman Cabai Merah (Antifungi Effectivity of Curcuma aeruginosa Extract to Colletotrichum capsici Pathogenicity on Red Chilli Pepper)

Prospects of Using Freons as Extractants of Curcuma Longa L. Root Essential Oil

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