Current applications and future perspectives of artificial nerve conduits

Jiang, Xu; Lim, Shawn; Mao, Hai‐Quan; Chew, Sing Yian

doi:10.1016/j.expneurol.2009.09.009

Cited by 353 publications

(261 citation statements)

References 168 publications

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“…Consequently, guidance of regenerating axons is improved by a mechanical effect but also by cellular growth factors, a chemical [6] and electrical cues [7] . Natural and synthetic biomaterials have been used as tube-guides, being the later sub-divided into two major groups: biodegradable and non-biodegradable [8,9] . The development of tube-guides is a consequence of the limitations inherent in the use of grafts, in terms of length, diameter and type of fiber, preventing damage to the sampling local, as it was previously referred [10] .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of biodegradable electric conductive tube-guides and mesenchymal stem cells

Ribeiro¹,

Pereira²,

Caseiro³

et al. 2015

WJSC

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Section: Introductionmentioning

confidence: 99%

Evaluation of biodegradable electric conductive tube-guides and mesenchymal stem cells

Ribeiro¹,

Pereira²,

Caseiro³

et al. 2015

WJSC

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“…Currently, a nerve autograft is the gold standard in treating nerve trunk defects when the tensionless apposition to suture the severed nerve is not possible. Nevertheless, autografts pose various drawbacks, such as the need for a secondary surgery, loss of donor site function, donor site morbidity, limited availability and structural differences between donor and recipient grafts [1,2]. Therefore, there is an urgent and unmet need to find an alternative approach in bridging the nerve defects.…”

mentioning

confidence: 99%

Collagen-Coated Polylactic-Glycolic Acid (PLGA) Seeded with Neural-Differentiated Human Mesenchymal Stem Cells as a Potential Nerve Conduit

Sulong¹,

Hassan²,

Ng³

et al. 2014

Adv Clin Exp Med

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Background. Autologous nerve grafts to bridge nerve gaps pose various drawbacks. Nerve tissue engineering to promote nerve regeneration using artificial neural conduits has emerged as a promising alternative. Objectives. To develop an artificial nerve conduit using collagen-coated polylactic-glycolic acid (PLGA) and to analyse the survivability and propagating ability of the neuro-differentiated human mesenchymal stem cells in this conduit. Material and Methods. The PLGA conduit was constructed by dip-molding method and coated with collagen by immersing the conduit in collagen bath. The ultra structure of the conduits were examined before they were seeded with neural-differentiated human mesenchymal stem cells (nMSC) and implanted sub-muscularly on nude mice thighs. The non-collagen-coated PLGA conduit seeded with nMSC and non-seeded non-collagen-coated PLGA conduit were also implanted for comparison purposes. The survivability and propagation ability of nMSC was studied by histological and immunohistochemical analysis. Results. The collagen-coated conduits had a smooth inner wall and a highly porous outer wall. Conduits coated with collagen and seeded with nMSCs produced the most number of cells after 3 weeks. The best conduit based on the number of cells contained within it after 3 weeks was the collagen-coated PLGA conduit seeded with neuro-transdifferentiated cells. The collagen-coated PLGA conduit found to be suitable for attachment, survival and proliferation of the nMSC. Minimal cell infiltration was found in the implanted conduits where nearly all of the cells found in the cell seeded conduits are non-mouse origin and have neural cell markers, which exhibit the biocompatibility of the conduits. Conclusions. The collagen-coated PLGA conduit is biocompatible, non-cytotoxic and suitable for use as artificial nerve conduits (Adv Clin Exp Med 2014, 23, 3, 353-362).

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“…Schwann cells) seems to be essential [11,12]. Various degradable materials are under investigation: e. g. Matrigel, Collagen, PLLA, PLGA, PCL [9,13,14]. However, tubular structures made of very soft or fast degrading materials might collapse or kink before regeneration has been completed and will strongly constrict or even obstruct tissue regeneration [15].…”

mentioning

confidence: 99%

Hollow fibers made from a poly(3-hydroxybutyrate)/poly-ε-caprolactone blend

Hinüber

Häußler²,

Vogel³

et al. 2011

Express Polym. Lett.

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Abstract. Since poly(3-hydroxybutyrate) (PHB) is inherently brittle and possesses poor elastic properties, hollow fibers produced by melt spinning from pure PHB, as described in our earlier study [Macromolecular Materials and Engineering, 2010, 295/6, 585-594], do not meet the required needs regarding the mechanical performance. Besides hardly available PHB copolymers, also blend systems are known to enhance material properties and have thus been considered to be eligible to fabricate flexible or rather pliable hollow fibers based on PHB. Blends of PHB and poly-!-caprolactone (PCL) are promising for the application in tissue engineering due to the inherent biocompatibility and biodegradability. A wide range of PHB/PCL compositions have been prepared by melt extrusion. Thermal and mechanical properties of the obtained specimens were analyzed in order to identify miscibility and degree of dispersion as well as to determine the influence on the overall mechanical performance. Even though these constituents are known to be immiscible, PHB/PCL 70/30 was proven to be an adequate composition. This blend showed a highly increased elongation and was found to be easily processable by melt spinning compared to pure PHB. From this blend well defined dimensionally stable bendable hollow fibers were fabricated.

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Current applications and future perspectives of artificial nerve conduits

Cited by 353 publications

References 168 publications

Evaluation of biodegradable electric conductive tube-guides and mesenchymal stem cells

Evaluation of biodegradable electric conductive tube-guides and mesenchymal stem cells

Collagen-Coated Polylactic-Glycolic Acid (PLGA) Seeded with Neural-Differentiated Human Mesenchymal Stem Cells as a Potential Nerve Conduit

Hollow fibers made from a poly(3-hydroxybutyrate)/poly-ε-caprolactone blend

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