Current development in microfluidic immunosensing chip

Henares, Terence G.; Mizutani, Fumio; Hisamoto, Hideaki

doi:10.1016/j.aca.2008.01.064

Cited by 201 publications

(143 citation statements)

References 103 publications

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“…As a promising technique, microfluidics is replacing the conventional diagnostic systems for diabetes screening due to its advantages of low reagent consumption, short analysis time and multi-process integration (Dittrich et al, 2006;Henares et al, 2008;Huang et al, 2007). In microfluidic systems, the traditional biochemical processes such as sample pretreatment, reagent transportation, mixing, reaction, separation, detection and product collection can be accomplished automatically on a single chip (Lin et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Development of microfluidic-based telemedicine for diabetes care and screening

Yao

Zhan

Hua

et al. 2012

Transactions of the Institute of Measurement and Control

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This paper describes a microfluidic-based telemedicine system for insulin detection and conveying the results digitally to physicians located off-site through the Internet. The communication infrastructure is designed to transfer the digital information from the assay site to established healthcare facilities where trained medical professionals can directly assist the detection process and provide diagnosis. The insulin detection device of the telemedicine system is an integrated polydimethysiloxane (PDMS) microfluidic device consisting of two pneumatic micropumps and one micromixer. The insulin detection protocol is based on microbeads-based double-antibody sandwich immunoassay coupled with luminal-hydrogen peroxide (H 2 O 2 ) chemiluminescence. A photometer detects the peak value of the luminous intensity, which indicates the insulin concentration of the patient plasma sample tested. The calibration curves of the insulin detection protocol have been quantified. The insulin detection limit of the microfluidic system is 4310 210 mol/l, which meets the common requirement of the current clinical studies of diabetes. Multiple immune indicators of diabetes can potentially be detected synchronously by the microfluidic system, thus providing physicians with integrative results necessary for accurate diagnosis via the Internet. The combination of microfluidic devices and telemedicine strategy offers new opportunities for diabetes care and screening, especially in rural areas where patients must travel long distances to physicians for healthcare information that might be obtained more cost effectively by local, less-trained personnel.

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Section: Introductionmentioning

confidence: 99%

Development of microfluidic-based telemedicine for diabetes care and screening

Yao

Zhan

Hua

et al. 2012

Transactions of the Institute of Measurement and Control

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“…It is, however, also time consuming and expensive to fabricate. 3 Polydimethylsiloxane (PDMS) on the other hand has allowed rapid strides forward in microfluidics due to its rapid, easy fabrication; optical clarity; UV transparency and biological inertness. 4,5 PDMS is the most extensively used polymeric material in the microfluidic community, and has been the material of choice for prototyping of microfluidic devices.…”

Section: Introductionmentioning

confidence: 99%

A rapid, inexpensive surface treatment for enhanced functionality of polydimethylsiloxane microfluidic channels

Beal¹,

Bubendorfer²,

Kemmitt³

et al. 2012

Biomicrofluidics

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A rapid, inexpensive method using alkoxysilanes has been developed to selectively coat the interior of polydimethylsiloxane (PDMS) microfluidic channels with an integral silicaceous layer. This method combines the rapid prototyping capabilities of PDMS with the desirable wetting and electroosmotic properties of glass. The procedure can be carried out on the open faces of PDMS blocks prior to enclosure of the channels, or by flowing the reagents through the preformed channels. Therefore, this methodology allows for high-throughput processing of entire microfluidic devices or selective modification of specific areas of a device. Modification of PDMS with tetraethoxysilane generated a stable surface layer, with enhanced wettability and a more stable electroosmotic flow rate than native PDMS. Modification of PDMS with 3-aminopropyltriethoxysilane generated a surface layer bearing amine functionalities allowing for further chemical derivatization of the PDMS surface.

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“…The high analytical efficiencies, fast processing times, reduced consumption of expensive biological reagents and portability make it suitable for a range of applications including diagnostics (46,52,(62)(63)(64)(65)(66)(67)(68)(69)(70), protein and cell-based high throughput drug screening (26,27,71,72) and biomolecule production through directed evolution (20). For example, microfluidic point-of-care diagnostic systems have gained much attention in recent years and proof of concept microfluidic diagnostic devices based on PCR (64,65), DNA/microRNA profiling (66,67), immunoassay (52,70), and protein profiling (69) have all been demonstrated. Soh and Colleagues developed a microfluidic device for genetic analysis of the H1N1 influenza virus from throat swab samples (64).…”

Section: Microfluidics In Real-world Applicationsmentioning

confidence: 99%