We characterize the spin Hall magnetoresistance (SMR), spin Seebeck effect (SSE) and damping-like spin-orbit torque (SOT) in thulium iron garnet/platinum bilayers with perpendicular magnetic anisotropy (PMA), by using harmonic Hall effect measurements. By consecutive annealing steps followed by measurements on a single device, we reveal that the spin-dependent effects gradually decrease in amplitude as the annealing temperature increases. We attribute this behavior primarily to the changes in the spin-mixing conductance which sensitively depends on the interface quality. However, further analysis demonstrates that, although the SSE scales closely with the SMR, the damping-like SOT shows a significantly different trend upon annealing, contrary to theoretical expectations. By comparing the damping-like SOT with the field-induced Hall effect we found evidence that scattering from Fe impurities in the Pt at the interface might be responsible for the distinct annealing temperature dependence of the damping-like SOT.