Current methodologies for the analysis of aminoglycosides

Stead, David

doi:10.1016/s0378-4347(00)00133-x

Cited by 205 publications

(163 citation statements)

References 125 publications

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“…However, these reagents are with drawbacks related to reagent stability, time consuming and detection sensitivity. For example, OPA is unstable and the elution of the OPA derivatives has been found to affect by the concentration of inorganic cations in the HPLC mobile phase [19,11].…”

Section: Discussionmentioning

confidence: 99%

“…Only chromatographic methods are capable of identifying and quantifying the individual components of the gentamicin complex. Gentamicin has no UV or visible absorbing chromophores and therefore cannot be detected by traditional techniques without derivatisation [11,12]. This necessitates gentamicin derivatization to allow its detection with the required sensitivity.…”

Section: Introductionmentioning

confidence: 99%

“…Either pre-or post-column derivatisation for fluorescence or UV detection can be implemented in this process. Gentamicin has been derivatised previously with O-phthalaldehyde (OPA), dansyl chloride, fluorescamine, 9-fluorenylmethyl chloroformate (FMOC-CI), 1-fluoro-2, 4-dinitrobenzene (DNFB) and 2,4,6-trinotrobenzenesulfonic acid (TNBS) [11].…”

Section: Introductionmentioning

confidence: 99%

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Pharmacokinetics of Gentamicin C after IV, IM, SC and Oral Administration , C , C 1 1a and C 2 in Broiler Chickens 2a

Abu‐Basha¹

2013

JBB

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The pharmacokinetics and bioavailability of four major gentamicin components (C 1 , C 1a , C 2 and C 2a ) in chicken plasma administered at 5 mg/kg body weight by different routes of administration (IV, IM, SC and oral) was determined using reversed-phase high performance liquid chromatography (RP-HPLC) and pre-column derivatization with Phenylisocyanate (PIC). All the components, except for C 1a were well absorbed (bioavailability of 60% or greater) following administration by the IM and SC routes. The bioavailability of C 1a was 58% and 35% following IM and SC administration, respectively. The apparent volume of distribution (V ss and Vd area ) for the C 1 component was significantly smaller than for any of the other components individually or combined. In addition, the C 1 component had a significantly shorter t½β and MRT following intravenous administration and a higher C max /Dose following intramuscular administration. This study showed significant differences in some pharmacokinetics parameters between four gentamicin components (C 1a , C 2a , C 1 and C 2 ) after administration of single mixture of gentamicin by different routes in chickens. The differences may have clinical and toxicological implications, and could explain the high variation in total gentamicin pharmacokinetics.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Pharmacokinetics of Gentamicin C after IV, IM, SC and Oral Administration , C , C 1 1a and C 2 in Broiler Chickens 2a

Abu‐Basha¹

2013

JBB

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“…As for the high performance liquid chromatography (HPLC) methods based on the UV detection, because of the lack of strong chromophores in the molecule strictures, pre-and post-column derivertizations become indispensable and poor derivertization yield and low stability of derivatives also are problems waiting to be resolved in many cases [7][8][9][10]. Moreover, many major and minor UV detectable ingredients in pharmaceutical intermediate and final products give rise to a lot of serious interferences to the detection of aminoglycoside antibiotics.…”

Section: Introductionmentioning

confidence: 99%

Comparative study on the analytical performance of three waveforms for the determination of several aminoglycoside antibiotics with high performance liquid chromatography using amperometric detection

Cai

Cheng³

et al. 2005

Journal of Chromatography A

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A preliminary comparative study was carried out on the analytical performances of a new six-potential waveform and other two detection waveforms, triple-potential waveform and quadruple-potential waveform. The analytical performances compared included signal response, background noise, signal/noise ratio and signal stability. Compared with triple-potential waveform and quadruple-potential waveform, the new six-potential waveform had higher signal response, signal/noise ratio, and sensitivity. As for determination reproducibility, the six-potential waveform also exhibited a slightly better performance than the other two waveforms. Under the selected experimental conditions based on the six-potential waveform, there is a linear correlation between peak area and concentration over two to three orders of magnitude for nine aminoglycoside antibiotics with a correlation coefficients better than 0.998 and the detection limits measured as three times the peak height signal-to-noise ratio for the nine aminoglycoside antibiotics were in the range of 0.0198-0.889 g/mL. The proposed method had been used to analyze real gentamicin sulphate drug sample.

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“…However, the use of aminoglycosides may cause sideeffects of ototoxicity and nephrotoxicity [20,21]. Careful monitoring of the aminoglycosides in plasma is routinely used as a guide to dosing, to prevent toxicity and to ensure efficacy.…”

mentioning

confidence: 99%

Silver-coated gold nanoparticles as concentrating probes and matrices for surface-assisted laser desorption/ionization mass spectrometric analysis of aminoglycosides

Wang

Liu

Wang

et al. 2009

J. Am. Soc. Mass Spectrom.

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A novel method for the determination of aminoglycosides by surface-assisted laser desorption/ ionization mass spectrometry (SALDI MS) with the aid of silver-coated gold nanoparticles (Au@AgNPs) has been developed. The Au@AgNPs with surface capped by anionic citrate were used as concentrating probes as well as matrices in SALDI MS. Adsorption of aminoglycosides onto the nanoparticles was mainly through electrostatic attraction. The aminoglycoside-adsorbed nanoparticles were directly characterized by SALDI MS after a simple washing. Using Au@AgNPs to preconcentrate the aminoglycosides from 500 L buffer solution, the limits of detection (LODs) at signal-to-noise ratio of 3 were 3, 25, 15, 30, and 38 nM for paromomycin, kanamycin A, neomycin, gentamicin, and apramycin, respectively. This method was successfully applied to the determination of aminoglycosides in human plasma samples. The LODs of aminoglycosides in plasma samples were 9, 130, 81, and 180 nM for paromomycin, kanamycin A, neomycin, and gentamicin, respectively. Recoveries of aminoglycosides in plasma samples were about 80%. (J Am Soc Mass Spectrom 2009, 20, 1925-1932) © 2009 American Society for Mass Spectrometry R ecently, surface-assisted laser desorption/ionization mass spectrometry (SALDI MS) has become a popular technique due to the rapid development of nanomaterials. Nanoparticles have been recognized as effective SALDI matrices because of their large surface area-to-volume ratio, ease of sample preparation, flexibility of sample deposition, and no dependence on the irradiation wavelength. Gold and Nile-red adsorbed gold nanoparticles were used as SALDI matrix for the mass spectrometric determination of aminothiols [7], and TiO 2 -coated magnetic nanoparticles [8] were also employed for the analysis of peptides and proteins. The mass range could be extended to 24 KDa with the use of nanoparticles as matrices, and the detection limit for peptide is about 20 fmol. In addition, titanium dioxide [9], silver [10], and silicon nanoparticles [11] successfully desorbed and ionized enediol compound, olefin, and small molecules, respectively. The use of nanoparticles as SALDI matrices not only eliminates matrix ion interference but also improves sample homogeneity. Sweet-spot searching is not required, therefore better shot-to-shot and sampleto-sample reproducibility is obtained.To improve the sensitivity and minimize the interferences of sample matrix, the nanoparticles were also used as concentrating probes to separate and concentrate the analytes from complex biologic samples before SALDI MS analysis. The surface of the nanoparticle was modified for the selective retention of analyte while removing interferences through simple washing. The collected analyte-adsorbed nanoparticles were either analyzed directly by SALDI/MS or mixed with conventional organic matrices, such as sinapinic acid (SA) and ␣-cyano-4-hydroxycinnamic acid (CHCA), for their matrix-assisted laser desorption/ionization (MALDI) MS analysis.

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Current methodologies for the analysis of aminoglycosides

Cited by 205 publications

References 125 publications

Pharmacokinetics of Gentamicin C after IV, IM, SC and Oral Administration , C , C 1 1a and C 2 in Broiler Chickens 2a

Pharmacokinetics of Gentamicin C after IV, IM, SC and Oral Administration , C , C 1 1a and C 2 in Broiler Chickens 2a

Comparative study on the analytical performance of three waveforms for the determination of several aminoglycoside antibiotics with high performance liquid chromatography using amperometric detection

Silver-coated gold nanoparticles as concentrating probes and matrices for surface-assisted laser desorption/ionization mass spectrometric analysis of aminoglycosides

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