Current progress in long-term and continuous cell metabolite detection using microfluidics

Shi, Jiaming; Tong, Lei; Tong, Wenqiang; Chen, Huaying; Lan, Minbo; Sun, Xiaoxi; Zhu, Yonggang

doi:10.1016/j.trac.2019.05.028

Cited by 45 publications

(38 citation statements)

References 142 publications

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“…[7][8][9] While most current in vitro monitoring methods detect individual metabolites or mitochondrial activity there are increasing efforts in detecting multiple metabolites in cell cultures. 10…”

Section: Introductionmentioning

confidence: 99%

Live monitoring of cellular metabolism and mitochondrial respiration in 3D cell culture system using NMR spectroscopy

Hertig

Maddah

Memedovski

et al. 2021

Analyst

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Section: Introductionmentioning

confidence: 99%

Live monitoring of cellular metabolism and mitochondrial respiration in 3D cell culture system using NMR spectroscopy

Hertig

Maddah

Memedovski

et al. 2021

Analyst

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“…Nevertheless, numerous papers have described the design of individual sensors for the detection of one or two of these parameters [23,24]. More recently, some papers have reported the development of a multiparametric sensing platform for the detection in vivo of metabolic species such as glucose, lactate, glutamate and oxygen [25][26][27]. However, the development of sensors for the detection of the selected metabolites (oxygen species (NO, H2O2) in addition to pH and lactate) in a multiparametric configuration combining the use of microfluidic technology have never been developed and reported.…”

Section: Introductionmentioning

confidence: 99%

Development of a multiparametric (bio)sensing platform for continuous monitoring of stress metabolites

Chmayssem

Verplanck

Tanase

et al. 2021

Talanta

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There is a growing need for real-time monitoring of metabolic products that could reflect cell damages over extended periods. In this paper, we report the design and development of an original multiparametric (bio)sensing platform that is tailored for the real-time monitoring of cell metabolites derived from cell cultures. Most attractive features of our developed electrochemical (bio)sensing platform are its easy manufacturing process, that enables seamless scale-up, modular and versatile approach, and low cost. In addition, the developed platform allows a multiparametric analysis instead of single-analyte analysis. Here we provide an overview of the sensors-based analysis of four main factors that can indicate a possible cell deterioration problem during cell-culture: pH, hydrogen peroxide, nitric oxide/nitrite and lactate. Herein, we are proposing a sensors platform based on thick-film coupled to microfluidic technology that can be integrated into any microfluidic system using Luer-lock connectors. This platform allows obtaining an accurate analysis of the secreting stress metabolites during cell/tissues culture.

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“…Classical methods for cell metabolite detection [116] such as cultivation of cells and intermittent cell or media culture collection present many disadvantages.…”

Section: Cell Metabolite Detectionmentioning

confidence: 99%

Progress in hydrogels for sensing applications: a review

Pinelli

Magagnin

Rossi

2020

Materials Today Chemistry

100

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There are several developments taking place in the field of sensors driven by the world today requirements. One of the most important novelties of the last two decades in the field is represented by the hydrogel-based sensors which constitute a wide family of innovative smart sensing devices relevant for many different applications. Hydrogels in fact are hydrophilic, biocompatible and highly water swellable polymer networks able to convert chemical energy into mechanical energy, with the great peculiarity to be able to respond to external stimuli. These characteristics have ensured them considerable recognition as valuable tool for smart sensing and diagnostics. The aim of this review is to focus on the advances obtained in the field in the last ten years.

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Current progress in long-term and continuous cell metabolite detection using microfluidics

Cited by 45 publications

References 142 publications

Live monitoring of cellular metabolism and mitochondrial respiration in 3D cell culture system using NMR spectroscopy

Live monitoring of cellular metabolism and mitochondrial respiration in 3D cell culture system using NMR spectroscopy

Development of a multiparametric (bio)sensing platform for continuous monitoring of stress metabolites

Progress in hydrogels for sensing applications: a review

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