Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification

Saragusty, Joseph; Arav, Amir

doi:10.1530/rep-10-0236

Cited by 431 publications

(325 citation statements)

References 187 publications

(168 reference statements)

Supporting

Mentioning

314

Contrasting

Unclassified

Order By: Relevance

“…Currently, slow freezing (classic) and vitrification (ultra-rapid) are the two main methods used commercially for IVEP embryo cryopreservation (Saragusty and Arav, 2011). Vitrification is the predominant technique used for IVEP (Dode et al, 2013) due to being a simple, fast and low-cost method (Sanches et al, 2016).…”

Section: Cryopreservation Of Bovine Embryosmentioning

confidence: 99%

See 1 more Smart Citation

Cryopreservation of in vitro-produced embryos: challenges for commercial implementation

Sanches¹,

Zangirólamo²,

Silva³

et al. 2017

Anim Reprod

View full text Add to dashboard Cite

In the last several years, the high demand for embryo production has resulted in the need to study new methods to make the cryopreservation of bovine embryos produced in vitro more efficient. Despite the advantages offered by in vitro embryo production (IVEP), the major challenge to its greater dissemination is to improve embryonic survival after cryopreservation. Embryos that are produced in vitro are less resistant to cryopreservation compared to those produced in vivo, which is due to the higher accumulation of lipids in their cells, among other factors. In this context, changes in the culture conditions such as the addition of lipolytic chemical substances and the adjustment of fetal calf serum in the medium have been proposed to decrease the lipid amount within the embryos. Several years ago, vitrification allowed good results for in vitro produced (IVP) embryos because of its simplicity, speed and low cost. More recently, another technique applied to simplify the embryo post-thawing rehydration step in vivo, direct transfer (DT), is a strategy that has proven to be of interest in helping to overcome limitations to the cryopreservation of in vitro produced embryos. DT has been performed by commercial laboratories, ensuring good embryo viability after thawing. However, commercial and operational limitations prevent the large-scale use of these techniques. Thus, this review aims to discuss the use of strategies to improve the postcryopreservation survival capacity and the aspects to be overcome so that the cryopreservation of IVP embryos becomes a well-established and commercially applicable technique in addition to presenting new guidelines for embryo transfer (ET) programs from a better selection of recipients.

show abstract

Section: Cryopreservation Of Bovine Embryosmentioning

confidence: 99%

“…Examples include the need for a qualified person to perform all stages of IVEP and the cryopreservation process, logistics between laboratory and recipients, as well as a trained technician in the field due the particularities of warming those cryopreserved embryos before transfer (Hasler, 2010;Saragusty and Arav, 2011).…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of in vitro-produced embryos: challenges for commercial implementation

Sanches¹,

Zangirólamo²,

Silva³

et al. 2017

Anim Reprod

View full text Add to dashboard Cite

show abstract

“…In this case, the short time of vitrification and elimination of costly programmable freezing equipment is of great importance. There are many modifications of freezing and vitrification procedures (Vajta and Kuwayama, 2006;Ledda et al, 2007;Pereira and Marques, 2008;Prentiz and Anzar, 2011;Saragusty and Arav, 2011).…”

Section: Cryopreservation Of Embryos and Oocytesmentioning

confidence: 99%

“…In the 1980s and 1990s, progeny was obtained after freezing and thawing of farm animal embryos. Also after vitrification of in vitro embryos, progeny of many farm animals has been obtained (Dobrinsky, 2002;Vajta and Kuwayama, 2006;Saragusty and Arav, 2011).…”

Section: Cryopreservation Of Embryos and Oocytesmentioning

confidence: 99%

Obtaining farm animal embryos <i>in vitro</i>

Duszewska¹,

Rapala²,

Trzeciak³

et al. 2012

J. Anim. Feed Sci.

View full text Add to dashboard Cite

This paper reviews the basic knowledge about obtaining farm animal embryos in vitro with special focus on differences among species and application of this procedure in the future. In vitro production of farm animal embryos consists of in vitro maturation (IVM) of oocytes, in vitro fertilization (IVF) of matured oocytes, and in vitro culture (IVC) of embryos. Oocytes can be collected from live animals (by laparotomy, laparoscopy, Ovum Pick Up) or from slaughtered ones (by puncture, sectioning). Usually immature oocytes are isolated, and during IVM they reach maturity. Matured oocytes are cultured with sperm (IVF), leading to the formation of zygotes. In the case of fertilization problems (horse, pig), intracytoplasmic sperm injection is used. The zygotes are usually cultured (IVC) to the morula and blastocyst stages. These embryos can be transferred to recipients or frozen/vitrified. Offspring have been obtained after transfer of cattle, sheep, goat, pig and horse embryos. This procedure can be used in animal breeding, biotechnology, medicine, and basic research.

show abstract

“…This method tends to be more effective than slow freezing for cryopreservation of in vitro produced (IVP) mammalian embryos, which are known to be more cryosensitive than embryos obtained in vivo (Nedambale et al, 2004;Saragusty and Arav, 2011). Introduction of the minimal volume cooling (MVC) principle further improved the survival rates after vitrification and led to the emergence of various opentype or surface method carriers based on the principle (Saragusty and Arav, 2011). Yet, vitrification is not routinely used in the livestock production.…”

Section: Introductionmentioning

confidence: 99%

A new simple and reliable vitrification device based on Hollow Fiber Vitrification (HFV) method evaluated using IVP bovine embryos

et al. 2017

View full text Add to dashboard Cite

A new vitrification device based on hollow fiber vitrification (HFV) was constructed using a glass capillary, which lead to simplified construction process and increased practicality of the device. The hollow fiber was attached to heat-pulled tip of the glass capillary using forceps. A protective sheath fitted on the capillary provided protection for the cellulose triacetate hollow fiber with loaded embryos and allowed safe storage in liquid nitrogen for long periods of time (2-12 month), transfer between tanks with liquid nitrogen and transportation within these tanks. No embryos were lost in the process. The device was tested using seven-dayold and eight-day-old IVP bovine blastocysts and expanded blastocysts as a model. Obtained survival (90% at 24 h post warming) and hatching rates (62% at 72 h post warming) of day 7 blastocysts and expanded blastocysts were comparable to those gained using various vitrification carriers. Vitrified embryos did not show an increase in the number of cells with damaged membrane or a decrease in total cell number per embryos in comparison to their non-vitrified counterparts. Day 7 and 8 expanded blastocysts did not differ significantly in terms of survival at 24 (97.01 vs. 97.50%) and 48 h post warming (95.52 vs. 95%), but showed significantly higher survival and hatching rates than day 7 and 8 blastocysts. These results indicated that high and repeatable survival rates can be obtained by selection of IVP bovine embryos at the developmental stage of expanded blastocyst for HFV. Further modification of the method may be required to achieve high and stable results with different developmental stages of IVP bovine embryo. The vitrification device presented in the current article may contribute to wider application of HFV method in livestock production.

show abstract

Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification

Cited by 431 publications

References 187 publications

Cryopreservation of in vitro-produced embryos: challenges for commercial implementation

Cryopreservation of in vitro-produced embryos: challenges for commercial implementation

Obtaining farm animal embryos <i>in vitro</i>

A new simple and reliable vitrification device based on Hollow Fiber Vitrification (HFV) method evaluated using IVP bovine embryos

Contact Info

Product

Resources

About