Current Progress in Sporothrix brasiliensis Basic Aspects

Gómez-Gaviria, Manuela; Martínez-Álvarez, José A.; Mora-Montes, Héctor M.

doi:10.3390/jof9050533

Cited by 9 publications

(5 citation statements)

References 131 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For decades, the murine model has been used as the in vivo gold standard model for pathogenicity mechanisms of distinct microorganisms [40], including Sporothrix spp [41]. However, recently, the scientific community has been showing ethical and social concerns, applied to the rationalization of animal model use [29,42,43].…”

Section: Discussionmentioning

confidence: 99%

Galleria mellonella Antimicrobial Peptides and Stress Management Gene Expression in Response to Deleterious Events Caused by Sporothrix brasiliensis

Reis,

De Jesus,

De Souza

et al. 2023

Preprint

View full text Add to dashboard Cite

Sporothrix brasiliensis is the most pathogenic species, responsible for the Brazilian cat-transmitted sporotrichosis hyperendemics. In this scenario, investigation of pathogen-host interaction can provide relevant information for future treatment strategies. To this end, the invertebrate Galleria mellonella has proven to be a suitable alternative to evaluate the virulence of pathogenic fungi since the insect immune system is similar to the mammalian innate immune response. The aim of this work was to investigate phenotypic and molecular aspects of the immune response of G. mellonella throughout the S. brasiliensis infection. Hemocyte density and the evolution of the fungal load were evaluated. In parallel, RT- qPCR expression analysis of genes encoding antimicrobial peptides (Gallerimycin and Galiomycin) and stress management genes (C7 Contig 15362 and C8 Contig 1910) was conducted. Fungal load and hemocyte densities were proportionally increasing simultaneously to the deleterious morphological events and larvae mortality. Gallerimycin, C7 Contig 15362, and C8 Contig 19101 genes were positively regulated (p&lt;0.05) at distinct moments of S. brasiliensis infection, characterizing a time-dependent and alternately modulated profile. Galiomycin gene expression remained unchanged. Our results contribute to the future proposal of potential alternative pathways to treat and, consequently, control S. brasiliensis zoonosis, a major public Health in Latin America.

show abstract

Section: Discussionmentioning

confidence: 99%

Galleria mellonella Antimicrobial Peptides and Stress Management Gene Expression in Response to Deleterious Events Caused by Sporothrix brasiliensis

Reis,

De Jesus,

De Souza

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…[41]. However, in recent times, the scientific community has been expressing ethical and social concerns towards the rationalization of animal model use [29,42,43].…”

Section: Discussionmentioning

confidence: 99%

Sporothrix brasiliensis Infection Modulates Antimicrobial Peptides and Stress Management Gene Expression in the Invertebrate Biomodel Galleria mellonella

Reis,

de Jesus,

de Souza

et al. 2023

JoF

Self Cite

View full text Add to dashboard Cite

Sporothrix brasiliensis is the most pathogenic species, responsible for the Brazilian cat-transmitted sporotrichosis hyperendemic. In this scenario, an investigation of the pathogen–host interaction can provide relevant information for future treatment strategies. To this end, the invertebrate Galleria mellonella has proven to be a suitable alternative for evaluating the virulence of pathogenic fungi, since the insect immune system is similar to the mammalian innate immune response. The aim of this work was to investigate phenotypic and molecular aspects of the immune response of G. mellonella throughout the S. brasiliensis infection. Hemocyte density and the evolution of the fungal load were evaluated. In parallel, RT-qPCR expression analysis of genes encoding antimicrobial peptides (Gallerimycin and Galiomycin) and stress management genes (C7 Contig 15362 and C8 Contig 19101) was conducted. The fungal load and hemocyte densities increased simultaneously and proportionally to the deleterious morphological events and larvae mortality. Gallerimycin, C7 Contig 15362 and C8 Contig 19101 genes were positively regulated (p < 0.05) at distinct moments of S. brasiliensis infection, characterizing a time-dependent and alternately modulated profile. Galiomycin gene expression remained unchanged. Our results contribute to the future proposal of potential alternative pathways for treating and consequently controlling S. brasiliensis zoonosis, a major public health issue in Latin America.

show abstract

“…Sporothrix spp. is a thermodimorphic, saprophytic fungus, which can contaminate humans and animals, causing sporotrichosis [1,2]. Infection occurs via traumatic inoculation by the bite or scratch of contaminated animals, mostly felines, or through inoculation of the fungus from contaminated organic material, such as soil, thorns, or wood [3,4].…”

Section: Introductionmentioning

confidence: 99%

Exploring Virulence Factors and Antifungal Susceptibility in<em> Sporothrix </em>Isolates: A Comparative Study between Feline and Human Hosts

Fernandes,

Caligiorne,

da Silva

et al. 2024

Preprint

View full text Add to dashboard Cite

Sporotrichosis’ clinical manifestations, lesions in the cutaneous or subcutaneous tissues, it is caused by dimorphic fungi of the Sporothrix complex, is a cosmopolitan mycosis that affects humans and animals. This study aimed to identify prevalent species in Minas Gerais, Brazil, and characterize virulence profiles and antifungal susceptibility associated with Sporothrix isolates. Isolates were identified using macro and micro-morphological traits as well molecular biology techniques. Antifungal susceptibility was determined using the Minimal Inhibitory Concentration test, while, virulence factors such as growth at different temperatures, biofilm formation, melanin production, and urease activity were assessed. Our findings revealed S. brasiliensis as a predominant species isolated. Urease activity was a crucial factor associated with growth at 50°C in S. brasiliensis isolates, highlighting their high thermotolerance. Furthermore, strains isolated from humans produced a more robust biofilm compare to those from felines. Strains of S. brasiliensis isolated from Felis catus exhibited faster melanin production, decreased susceptible to antifungal agents tested, and superior growth capacity at 50°C to strains isolated from humans. Therefore, our study highlights the prevalence of S. brasiliensis causing sporotrichosis in both human and felines. However, it underscores the circulation of different strains, with varying levels of virulence and susceptibility to antifungal agents.

show abstract

Current Progress in Sporothrix brasiliensis Basic Aspects

Cited by 9 publications

References 131 publications

Galleria mellonella Antimicrobial Peptides and Stress Management Gene Expression in Response to Deleterious Events Caused by Sporothrix brasiliensis

Galleria mellonella Antimicrobial Peptides and Stress Management Gene Expression in Response to Deleterious Events Caused by Sporothrix brasiliensis

Sporothrix brasiliensis Infection Modulates Antimicrobial Peptides and Stress Management Gene Expression in the Invertebrate Biomodel Galleria mellonella

Exploring Virulence Factors and Antifungal Susceptibility in<em> Sporothrix </em>Isolates: A Comparative Study between Feline and Human Hosts

Contact Info

Product

Resources

About