Current Status of and Perspectives on the Application of Marmosets in Neurobiology

Okano, Hideyuki

doi:10.1146/annurev-neuro-030520-101844

Cited by 74 publications

(60 citation statements)

References 148 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additional cortical surface projection maps and different gene combinations, which will be provided on the MGA site, have the potential to delineate further primate-specific cortical areas, providing insights into primate-specific cortical function and connectivity. Together with technologies such as microarrays, multiscale RNA-seq, and high-resolution human brain mapping by MRI, DTI, and DMN, the marmoset ISH database provides an invaluable reference tool that helps translate knowledge from rodents into primates and advance primate molecular neurobiology research ( 55 ).…”

Section: Discussionmentioning

confidence: 99%

Cellular-resolution gene expression profiling in the neonatal marmoset brain reveals dynamic species- and region-specific differences

Kita

Nishibe

Wang

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Precise spatiotemporal control of gene expression in the developing brain is critical for neural circuit formation, and comprehensive expression mapping in the developing primate brain is crucial to understand brain function in health and disease. Here, we developed an unbiased, automated, large-scale, cellular-resolution in situ hybridization (ISH)–based gene expression profiling system (GePS) and companion analysis to reveal gene expression patterns in the neonatal New World marmoset cortex, thalamus, and striatum that are distinct from those in mice. Gene-ontology analysis of marmoset-specific genes revealed associations with catalytic activity in the visual cortex and neuropsychiatric disorders in the thalamus. Cortically expressed genes with clear area boundaries were used in a three-dimensional cortical surface mapping algorithm to delineate higher-order cortical areas not evident in two-dimensional ISH data. GePS provides a powerful platform to elucidate the molecular mechanisms underlying primate neurobiology and developmental psychiatric and neurological disorders.

show abstract

Section: Discussionmentioning

confidence: 99%

Cellular-resolution gene expression profiling in the neonatal marmoset brain reveals dynamic species- and region-specific differences

Kita

Nishibe

Wang

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Non-human primate (NHP) neuroimaging provides a granular view of the evolution of the brain connectome (Thiebaut de Schotten et al, 2019) and overcomes constraints of human neuroimaging by accessing "ground truth" data from neuronal tracing (Buckner and Margulies, 2019). As one of the smallest NHP with many practical advantages and potentials in biomedical research, the common marmoset (Callithrix jacchus) has drawn considerable interest for NHP neuroimaging research (Eliades and Miller, 2017;Homman-Ludiye and Bourne, 2017;Matsuzaki and Ebina, 2020;Mitchell and Leopold, 2015;Okano, 2021;Okano and Mitra, 2015;Schultz-Darken et al, 2016;Walker et al, 2017). Via Marmoset Brain Mapping (marmosetbrainmapping.org) and the Marmoset Brain Connectivity Atlas (marmosetbrain.org), we have contributed the ultra-high-resolution ex-vivo diffusion MRI data , mesoscale neural tracing data (Majka et al, 2020), and structural atlases , which have enabled an unprecedented level of precision in analyses of the marmoset brain neuroanatomy.…”

Section: Introductionmentioning

confidence: 99%

An integrated resource for functional and structural connectivity of the marmoset brain

Tian

Chen

Majka

et al. 2021

Preprint

View full text Add to dashboard Cite

The comprehensive integration of structural and functional connectivity data is required for the accurate modeling of brain functions. While resources for studying structural connectivity of non-human primate marmoset brains already exist, their integration with functional connectivity data has remained unavailable. Therefore, we present a comprehensive resource for marmoset brain mapping, which integrates the largest awake resting-state fMRI dataset to date (39 marmosets, 709 runs, and 12053 mins), cellular- level neuronal-tracing dataset (52 marmosets and 143 injections), and multi-resolution diffusion MRI dataset. The combination of these data into the same MRI space allowed us to 1). map the fine-detailed functional networks and cortical parcellations; 2). develop a deep-learning-based parcellation generator to preserve the topographical organization of functional connectivity and reflect individual variabilities; 3). investigate the structural basis underlying functional connectivity by computational modeling. Our resource will broadly model the marmoset brain architecture and facilitate future comparative and translational studies of primate brains.

show abstract

“…To faithfully reproduce these neurological diseases with human-like behavioral symptoms, there are pressing needs to develop animal models using non-human primates (Verdier et al, 2015;Qiu and Li, 2017). The common marmosets (Callithrix jacchus), a new world monkey, has been proposed as a possible primate model for these diseases because of their availability in transgenic tools (Sasaki et al, 2009;Izpisua Belmonte et al, 2015;Park et al, 2016), their smooth brain surface for pinpoint intracranial injection (Lin et al, 2019;Mundinano et al, 2016) and their ability to be trained on behavioral paradigms (Okano, 2021). The marmosets can learn to perform a variety of visual and cognitive tasks under head unrestrained conditions (Spinelli et al, 2004;Clarke et al, 2011;Yamazaki et al, 2011;Jendritza et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Dissecting errors made in response to externally- and internally-driven visual tasks in the common marmosets and humans

Amly

Chen

Onoe

et al. 2021

Preprint

View full text Add to dashboard Cite

Various saccadic tasks traditionally used in oculomotor research, including both exogenously-driven and endogenously-driven saccades, have been proposed as clinical diagnostic for human movement disorders. Recently, common marmosets have been proven to be a good primate model for these movement disorders. However, whether similar saccadic measurements can be used for marmosets was not tested. Here, we trained three marmosets on the gap task, an exogenously-driven saccadic task, and the oculomotor delayed response (ODR) task, an endogenously-driven saccadic task. We demonstrated that with long-term training, they were able to learn and switch between the two tasks. The marmosets showed undershooting tendency in the gap and both under- and overshooting tendency in the ODR task when they made saccades to the target. We also categorized the error trails into distractive, impulsive, and visuomotor errors, depending on when a false saccade happened in relation to the task progression and interpreted possible causes of errors based on the saccade response time and the task history. Each error category may indicate specific failures in a particular aspect of cognitive or sensory-motor processes. Finally, and critically, we found that saccades from successful trials exhibited the highest saccade peak velocity and the shortest saccade duration comparing to the same saccade amplitude from error trials. Taken together, we showed the potential of training the same marmosets on conflicting oculomotor tasks simultaneously. We documented the baseline performance and compared successful and error trials in both gap and ODR tasks from the same animals. We propose that analyzing the error trials in sync with successful trials could provide a further understanding of the cognitive and sensorimotor processes of the marmosets, in both healthy and disease conditions.

show abstract

Current Status of and Perspectives on the Application of Marmosets in Neurobiology

Cited by 74 publications

References 148 publications

Cellular-resolution gene expression profiling in the neonatal marmoset brain reveals dynamic species- and region-specific differences

Cellular-resolution gene expression profiling in the neonatal marmoset brain reveals dynamic species- and region-specific differences

An integrated resource for functional and structural connectivity of the marmoset brain

Dissecting errors made in response to externally- and internally-driven visual tasks in the common marmosets and humans

Contact Info

Product

Resources

About