Current status of genetic modulation of growth factors in wound repair (Review)

Riedel, K.; Riedel, Frank; Goessler, Ulrich Reinhart; Holle, Gisbert; Germann, Günter; Sauerbier, Michael

doi:10.3892/ijmm.17.2.183

Cited by 11 publications

(9 citation statements)

References 109 publications

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“…platelet‐derived growth factor, epidermal growth factor and transforming growth factor‐β) and the non healing phase [e.g. tumour necrosis factor alpha (TNFα), interleukin (IL)‐6 or IL‐1] of chronic ulcers are in the focus of pathophysiological investigations (6–11), no objective laboratory parameter is available to assess and monitor the status of inflammation for clinical practice. Therefore, the present pilot study aimed to prove the significance of levels of IL‐6 and TNFα in wound washouts for assessing potent inflammatory triggers like bacterial bioburden, specific pathogens and a polymicrobial infection.…”

Section: Introductionmentioning

confidence: 99%

Interleukin-6 concentrations in wound fluids rather than serological markers are useful in assessing bacterial triggers of ulcer inflammation

Ambrosch

Lobmann

Pott

et al. 2008

International Wound Journal

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Bacterial pathogenicity, microbial load and diversity are decisive for outcome and therapy of non healing ulcers. However, until now, no routine laboratory parameter is available to assess the inflammatory level caused by chronic wound infections. We thus investigated the usefulness of levels of interleukin (IL)-6 and tumour necrosis factor alpha (TNFalpha) in wound fluids for assessing ulcer inflammation in the presence or absence of microbial triggers. In addition, the predictive values of local cytokine analyses were compared with those of C-reactive protein (CRP) and lipopolysaccharide-binding protein (LBP) because serological markers are normally used to underline the suspicion of wound infections. The present data from chronic arterial and venous ulcers (n = 45) clearly show that mixed bacterial infections increased IL-6 and TNFalpha concentrations in wound fluids when compared with ulcers with a monomicrobial infection (P < 0.01 and P < 0.05, respectively). IL-6 was also significantly elevated when a high bacterial load [versus <10(5) colony-forming units (cfu)/ml: P = 0.04] or an infection with Pseudomonas was observed (versus isolation of non Pseudomonas strains: P = 0.05). Although distinct proinflammatory triggers may interfere with regard to cytokine levels, sensitivity and specificity were significant in predicting bacterial risk factors, particularly for IL-6 at a designated cut-off of 125 pg/ml (sensitivity and specificity for predicting a mixed infection: 70% and 64%, for predicting a bacterial load of >10(5) cfu/ml: 62% and 57% and for predicting an infection with Pseudomonas: 90% and 57%). In contrast to local cytokine levels, the serological markers CRP and LBP were not associated with the presence of any of the investigated bacterial triggers. Focusing on the aim of the study, IL-6 analysed in wound washouts seems to be a useful diagnostic marker for a sensitive and specific assessment of ulcers inflammation with regard to bacterial triggers.

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Section: Introductionmentioning

confidence: 99%

Interleukin-6 concentrations in wound fluids rather than serological markers are useful in assessing bacterial triggers of ulcer inflammation

Ambrosch

Lobmann

Pott

et al. 2008

International Wound Journal

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“…Growth factors as well as other soluble mediators are crucial in orchestrating all stages of wound healing, being both autocrine and paracrine mediators 124. Novel therapeutic approaches have been trying to attenuate the interactions between various wound cell types, extracellular matrix molecules, and soluble mediators 125.…”

Section: Resultsmentioning

confidence: 99%

Wound‐healing modulation in upper airway stenosis—Myths and facts

2008

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Wound healing plays a major role in the development of acquired subglottic stenosis. Pharmacologic treatment of subglottic stenosis must address both physiologic and pathologic healing processes. The relevant Pubmed and Ovid databases from 1960 to 2007 were systematically searched. Several modulating agents have been tested. Most of them were poorly investigated. Three modalities were thoroughly studied-steroids and antibiotics, mitomycin, and antireflux medications. However, there are conflicting data regarding their role in preventing and treating subglottic stenosis. Current data support to some extent the textbook suggestions of antibiotics, steroids, and antireflux treatment. As no other treatment options exist, we recommend using these modalities for pharmacologic modulation of subglottic stenosis. Mitomycin should still be considered as an unproven treatment; its use may be considered as an adjunct. V

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“…Antisense gene technology is a new method developed in recent years, which includes antisense DNA,antisense RNA, and ribozyme. These techniques have been widely studied recently because of good specificity of desired effect, and low cytotoxicity [14][15][16] . Zhang, et al .…”

Section: Discussionmentioning

confidence: 99%

T Cell Apoptosis Was Inhibited by Fas of Antisense Oligonucleotide

Zheng¹,

Jiang²,

Ge³

et al. 2010

2010 4th International Conference on Bioinformatics and Biomedical Engineering

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We investigate the effect of specific antisense oligodeoxynucleotide (ASO DN) inhibition of Fas expression on T cell apoptosis induced by gastric carcinoma cell . Fas receptor (Fas) and Fas ligand (FasL) expressed by the gastric carcinoma cell line SGC-7901 and Jurkat T cells were detected by flow cytometry (FCM) and the ability of FasL-inducing T cell apoptosis was tested by co-culture assay in vitro with SGC-7901 cells and Jurkat T cells. The Jurkat cells were transfected with Fas-ASODN using lipofectin, and the effects of Fas-ASODN on Fas mRNA level, Fas expression on T cells surface, and apoptosis were investigated by RT-PCR, FCM and co-culture assay, respectively. SGC-7901 cells expressing functional FasL could induce the apoptosis of Jurkat cells as demonstrated by co-culture assays. After the Jurkat cells were transfected with Fas ASODN, the level of Fas mRNA, the expression rate of Fas and the apoptotic rate induced by gastric carcinoma cells were all decreased. Gastric carcinoma cells expressing FasL can induce apoptosis in Fas-expressing T cells, indicating that transfection of Fas ASODN can partially convert the immune inhibitory condition induced by gastric carcinoma cells. Fas ASODN may be a useful tool in the armamentarium of tumor immune therapy.

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Current status of genetic modulation of growth factors in wound repair (Review)

Cited by 11 publications

References 109 publications

Interleukin-6 concentrations in wound fluids rather than serological markers are useful in assessing bacterial triggers of ulcer inflammation

Interleukin-6 concentrations in wound fluids rather than serological markers are useful in assessing bacterial triggers of ulcer inflammation

Wound‐healing modulation in upper airway stenosis—Myths and facts

T Cell Apoptosis Was Inhibited by Fas of Antisense Oligonucleotide

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