Current status of genetic transformation technology developed in cucumber (Cucumis sativus L.)

Wang, Shunli; Ku, Seong Sub; Ye, Xingguo; He, Congfen; Kwon, Suk‐Yoon; Choi, Pil Son

doi:10.1016/s2095-3119(14)60899-6

Cited by 40 publications

(42 citation statements)

References 70 publications

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“…However, the exact functions and biological significance of these TPS genes in cucumber will need to be elucidated by gain-or loss-offunction approaches like those used in model plants such as Arabidopsis (Tholl et al, 2005). Recently reported transformation protocols for cucumber should facilitate the investigation of the enzymatic hypotheses generated from this study (Nanasato et al, 2013;Wang et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Integrative Analyses of Nontargeted Volatile Profiling and Transcriptome Data Provide Molecular Insight into VOC Diversity in Cucumber Plants (Cucumis sativus)

et al. 2016

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Plant volatile organic compounds, which are generated in a tissue-specific manner, play important ecological roles in the interactions between plants and their environments, including the well-known functions of attracting pollinators and protecting plants from herbivores/fungi attacks. However, to date, there have not been reports of holistic volatile profiling of the various tissues of a single plant species, even for the model plant species. In this study, we qualitatively and quantitatively analyzed 85 volatile chemicals, including 36 volatile terpenes, in 23 different tissues of cucumber (Cucumis sativus) plants using solid-phase microextraction combined with gas chromatography-mass spectrometry. Most volatile chemicals were found to occur in a highly tissue-specific manner. The consensus transcriptomes for each of the 23 cucumber tissues were generated with RNA sequencing data and used in volatile organic compound-gene correlation analysis to screen for candidate genes likely to be involved in cucumber volatile biosynthetic pathways. In vitro biochemical characterization of the candidate enzymes demonstrated that TERPENE SYNTHASE11 (TPS11)/TPS14, TPS01, and TPS15 were responsible for volatile terpenoid production in the roots, flowers, and fruit tissues of cucumber plants, respectively. A functional heteromeric geranyl(geranyl) pyrophosphate synthase, composed of an inactive small subunit (type I) and an active large subunit, was demonstrated to play a key role in monoterpene production in cucumber. In addition to establishing a standard workflow for the elucidation of plant volatile biosynthetic pathways, the knowledge generated from this study lays a solid foundation for future investigations of both the physiological functions of cucumber volatiles and aspects of cucumber flavor improvement.

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Section: Discussionmentioning

confidence: 99%

Integrative Analyses of Nontargeted Volatile Profiling and Transcriptome Data Provide Molecular Insight into VOC Diversity in Cucumber Plants (Cucumis sativus)

et al. 2016

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“…In general, target genes are transferred into dicotyledonous plant genomes using the Agrobacterium-mediated transformation approach (Wang et al, 2015). Success of Agrobacterium-mediated transformation depends upon the regeneration frequency and number of shoots, which, in turn, depend upon the explant types, pre-cultivation time, acetosyringone concentration, infection time and cocultivation time.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, genetic engineering has some clear advantages such as a short breeding time and no changes in the desirable characteristics of plants, therefore, it is particularly valuable in genetic improvement of cucumber (Wang et al, 2015). During the last three decades, cucumber transformation techniques have undergone advances.…”

Section: Introductionmentioning

confidence: 99%

Establishment of a High-efficiency Genetic Transformation System of Cucumber (Cucumis sativus) using Csexpansin 10 (CsEXP10) Gene

Sun¹,

Luo²,

Li³

et al. 2017

IJAB

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Cucumber is one of the most important vegetable crops in the world. To establish an efficient genetic transformation system in cucumber, the role of different factors such as pre-cultivation time, acetosyringone concentration, infection time and cocultivation time that can influence the transformation, was evaluated. In addition, Csexpansin 10 (CsEXP10) gene was transformed into the cucumber genome to produce transgenic lines. The results showed that various optimal parameters such as 100 mg L -1 of kanamycin concentration for selection of transformants, 2 d of pre-cultivation time, 100 μmol L -1 of acetosyringone concentration, 15 min of infection time and 2 d of co-cultivation time were obtained using cotyledonary node explants. The rooting frequency observed on Murashige and Skoog (MS) medium supplemented with 0.2 mg L -1 indole acetic acid and 400 mg L -1 cefotaxime was found to be 100.00%. The positive transgenic cucumber lines were identified using PCR analysis and GUS staining assay. It suggests that the genetic transformation system developed using cotyledonary node explants is efficient and successful in cucumber.

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“…In the present study, multiple shoots developed on BA-containing media failed to elongate in the same medium. This result additionally suggested that genotype is considered as one of the main factors influencing the shoot bud regeneration rate and various genotypes needed different growth regulator combinations for plantlet development (Wang et al, 2015). The number of multiple shoots was enhanced in a medium supplemented with BA alone and/or in combination with a lower dose of auxins to the highest value of 94%.…”

Section: Effect Of Ms Strength and Ga 3 Concentration On Shoot Elongamentioning

confidence: 87%

Lofty frequency and reproducible plant regeneration from mature nodal explants of ‘‘Egusi’’melon (Citrullus colocynthis L.)

Thangavel¹,

Natarajan²,

Shanmugam³

et al. 2019

bta

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The present study attempts to explain a mass culture protocol for large scale multiple shoot bud regeneration from nodal explants of Citrullus colocynthis L. The highest shoot bud induction (94.9%) without attaining callus was observed in Murashige and Skoog's (MS) medium supplemented with benzyl amino purine (BA)in which the combination of benzyl amino purine BAP (1.0 mg/l), kinetin (0.5 mg/l), gibberellic acid (1.5 mg/l) produced more shoots/explants. This study strongly suggests that the pre-eminence of BA and other cytokinins in combination with GA 3 was found to be essential for a swift multiple shoot bud induction as well as an enhanced rate of shoot proliferation in C. colocynthis. A full-strength MS medium, supplemented with indole 3 butyric acid IBA (1.5 mg/l) combined with BA (1.5 mg/l), had the maximum rooting response and the regenerated plantlets were successfully established in the field with an 80% survival rate. : +91 9994112073 P. Thangavel, S. Natarajan, V. Shanmugam et al.

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Current status of genetic transformation technology developed in cucumber (Cucumis sativus L.)

Cited by 40 publications

References 70 publications

Integrative Analyses of Nontargeted Volatile Profiling and Transcriptome Data Provide Molecular Insight into VOC Diversity in Cucumber Plants (Cucumis sativus)

Integrative Analyses of Nontargeted Volatile Profiling and Transcriptome Data Provide Molecular Insight into VOC Diversity in Cucumber Plants (Cucumis sativus)

Establishment of a High-efficiency Genetic Transformation System of Cucumber (Cucumis sativus) using Csexpansin 10 (CsEXP10) Gene

Lofty frequency and reproducible plant regeneration from mature nodal explants of ‘‘Egusi’’melon (Citrullus colocynthis L.)

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