Current status of Pseudomonas cepacia typing systems

Rabkin, Charles S.; Jarvis, William R.; Martone, William J.

doi:10.1007/bf00145643

Cited by 11 publications

(5 citation statements)

References 21 publications

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“…However, bacteriocin typing does not provide the reproducibility required for epidemiologic assessments, and its greater technical complexity also limits its usefulness for characterization of epidemic strains of P. cepacia (27). A number of serotyping systems using either heat-killed or heat-stable antigens of P. cepacia have been reported (10,21,26,27). A French group (13) serotyped 285 isolates by using a combination of seven O (somatic) and five H (flagellar) antisera.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, epidemiological studies have successfully used DNA analysis. Plasmid analysis has been used in outbreaks of P. cepacia infections that have involved plasmid-bearing strains (27). However, the frequency of plasmid carriage in the overall P. cepacia population has been estimated to be as low as 10%, which seriously limits the use of this technique as a general analytic tool (7).…”

Section: Discussionmentioning

confidence: 99%

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Arbitrarily primed polymerase chain reaction as a rapid method to differentiate crossed from independent Pseudomonas cepacia infections in cystic fibrosis patients

et al. 1993

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We used DNA fingerprinting by the arbitrarily primed polymerase chain reaction (AP-PCR) technique for an epidemiological investigation of 23 Pseudomonas cepacia isolates obtained from 11 cystic fibrosis (CF) patients attending our CF center. This approach was compared with ribotyping, pulsed-field gel electrophoresis (PFGE), and conventional phenotypic typing. AP-PCR and ribotyping were identical in resolving power, since the two methods generated four different profiles and identified the same group of strains. Six patients on the one hand and four on the other harbored strains of the same genotype, thus raising the possibility of either patient-to-patient transmission or acquisition from a common hospital environmental source. PFGE results were in good agreement with those of the other two methods, but PFGE seems more discriminative since it

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Arbitrarily primed polymerase chain reaction as a rapid method to differentiate crossed from independent Pseudomonas cepacia infections in cystic fibrosis patients

et al. 1993

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“…Several papers have called for methods for subdivision of the species that are of epidemiological utility (Gilardi, 1983;Holmes, 1986;Rabkin, Jarvis & Martone, 1987;Rabkin et al, 1989). Many metabolic characteristics are found to be quite variable within the species and schemes have been constructed designating subdivisions based on this variability.…”

Section: Introductionmentioning

confidence: 99%

Distribution of Burkholderia cepacia phenotypes by niche, method of isolaiton and pathogenicity of onion

Yohalem

Lorbeer

1997

Annals of Applied Biology

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Several proposals have been made for the subdivision of the bacterial species Burkholderia cepacia. Data applying these schemes to a collection of 188 strains of B. cepacia, isolated using a variety of methods, from soils, decayed onions, and nosocomial sources are presented. None of the schemes could be shown to be associated with the method of isolation, niche from which a strain was isolated, pectolytic activity, or pathogenicity to onion. Strains isolated by serial dilution on onion slices were much more uniform than were strains isolated using semiselective media. The nutritionally stringent medium of Lumsden and Sasser (US Patent No. 4 588 584) was judged better for the collection of a phenotypically broad sample of B. cepacia than those of Hagedorn, Gould, Bardinelli & Gustavson (1987) and Wu & Thompson (1984), which are based on antibiotic insensitivity. Pectolytic activity and pathogenicity to onion were shown to be highly correlated characters. None of the strains of clinical origin was capable of macerating onion tissue. 277. LA: The Station. Wu B J, Thompson S T. 1984. Selective medium for Pseudomonas cepacia containing 9-chloro-9-(4-diethylaminopheny1)-10-phenylacridan and polymyxin B sulfate. Applied Environmental Microbiology 48:743-746. Yohalem D S. 1993. Characterization of diversity among Pseudomonas cepacia strains isolated from soils, decayed onions, and cEinical sources. Ph.D. Thesis. Cornell University, Ithaca, NY. Yohalem D S, Lorbeer J W. 1994a. Multilocus isoenzyme diversity among strains of Pseudomonas cepacia isolated from decayed onions, soils, and clinical sources. Systematic and Applied Microbiology 17: 116-124. Yohalem D S, Lorbeer J W. 1994. Intraspecific metabolic diversity among strains of Burkholderia cepacia isolated from decayed onions, soils, and the clinical environment. Antonie van Leeuwenhoek 399-1 5. 65: 1 1 1-1 3 1.

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“…A number of methods have been used to establish relationships between B. cepacia complex isolates, including phenotypic assays, such as serotyping, antimicrobial susceptibility typing, bacteriocin typing, and biotyping (33,34). In recent years, phenotypic methods have been largely replaced by genotypic methods, including macrorestriction digestion of chromosomal DNA followed by pulsed-field gel electrophoresis (PFGE) and various PCR-based fingerprinting techniques (2,31,43,44,48).…”

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Comparative Assessment of Genotyping Methods for Epidemiologic Study of Burkholderia cepacia Genomovar III

et al. 2002

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We analyzed a collection of 97 well-characterized Burkholderia cepacia genomovar III isolates to evaluate multiple genomic typing systems, including pulsed-field gel electrophoresis (PFGE), BOX-PCR fingerprinting and random amplified polymorphic DNA (RAPD) typing. The typeability, reproducibility, and discriminatory power of these techniques were evaluated, and the results were compared to each other and to data obtained in previous studies by using multilocus restriction typing (MLRT). All methods showed excellent typeability. PFGE with SpeI was more reproducible than RAPD and BOX-PCR fingerprinting. The discriminatory power of the methods was variable, with PFGE and RAPD typing having a higher index of discrimination than BOX-PCR fingerprinting. In general, the results obtained by PFGE, BOX-PCR fingerprinting, and MLRT were in good agreement. Our data indicate that different genomic-based methods can be used to type B. cepacia genomovar III isolates depending on the situation and the epidemiologic question being addressed. PFGE and RAPD fingerprinting are best suited to addressing small-scale studies (i.e., local epidemiology), whereas BOX-PCR fingerprinting is more appropriate for large-scale studies (i.e., global epidemiology). In this regard, BOX-PCR fingerprinting can be considered a rapid and easy alternative to MLRT.Cystic fibrosis (CF) is the most common hereditary disease in Caucasian populations. Clinical manifestations of CF result from a disturbance in electrolyte transport that primarily affects the respiratory and digestive systems. The CF lung is particularly susceptible to infection with a variety of opportunistic bacteria (9, 14), and exacerbations of chronic infection cause significant morbidity and mortality (36). Among the bacterial species capable of causing infection in CF are those belonging to the Burkholderia cepacia complex, which is currently comprised of nine closely related genomic species or genomovars (11,21,45). Recent work has demonstrated that the majority of infected CF patients harbor either B. cepacia genomovar III or Burkholderia multivorans (genomovar II) (1,25,38). Furthermore, limited data suggest that B. cepacia genomovar III (or perhaps certain specific strains within genomovar III) may be relatively more virulent than other species in this complex (3,12,19).The broad-spectrum antimicrobial resistance, absence of a vaccine, and virulence of certain strains have made prevention of B. cepacia complex infection an important goal in CF patient care (21, 22). However, much still remains unknown regarding the epidemiology of infection in CF. A number of previous studies have demonstrated transmission of B. cepacia complex strains between persons with CF (for reviews, see references 15, 16, 21, and 22). More recent studies indicate that the natural environment is also a likely reservoir for acquisition of B. cepacia complex strains (4, 24). Better risk assessment of potential sources of infection and the development of optimal infection control policies rely on a more complet...

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Current status of Pseudomonas cepacia typing systems

Cited by 11 publications

References 21 publications

Arbitrarily primed polymerase chain reaction as a rapid method to differentiate crossed from independent Pseudomonas cepacia infections in cystic fibrosis patients

Arbitrarily primed polymerase chain reaction as a rapid method to differentiate crossed from independent Pseudomonas cepacia infections in cystic fibrosis patients

Distribution of Burkholderia cepacia phenotypes by niche, method of isolaiton and pathogenicity of onion

Comparative Assessment of Genotyping Methods for Epidemiologic Study of Burkholderia cepacia Genomovar III

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