Current status of the metabolic engineering of microorganisms for biohydrogen production

From the Ancient Tribes to Modern Societies, Microalgae Evolution from a Simple Food to an Alternative Fuel Source

“…(2) the relation of inhibitors with hydrogen production mechanism; (3) microalgae speciesspecific hydrogen production and screening of certain strains as hydrogen-producing machineries; (4) genetic basis of hydrogen production both in terms of enzymes and mechanistic models; (5) regulation of cell metabolism to shift their storage metabolism; (6) the effect of nutrition to PSII system; (7) evaluation of biophotolysis; (8) genetic engineering tools for an efficient production; (9) commercialization and scale-up; (10) immobilization for sustainable biohydrogen production; and (11) evolutionary development of hydrogen-producing machinery, which were highlighted in a series of comprehensive reports (Ghirardi et al, 1997;Wykoff et al, 1998;Melis et al, 2000;Kosourov et al, 2002;Forestier et al, 2003;Posewitz et al, 2004;Ghirardi et al, 2005;Kruse et al, 2005;Meyer, 2007;Laurinavichene et al, 2008;Kosourov and Seibert, 2009;Matthew et al, 2009;Faraloni and Torzillo, 2010;Gaffron, 1939;Kruse and Hankamer, 2010;Oh et al, 2011;Srirangan et al, 2011;Meuser et al, 2012;Oncel and Sabankay, 2012;Scoma et al, 2012;Oncel, 2013;Torzillo and Seibert, 2013;Oncel and Kose, 2014).…”

“…The biohydrogen metabolism within the cell is composed of complex reactions, and according to the previous studies, each approach in molecular metabolism gives positive results to upgrade biohydrogen studies. The following studies have been done: starch metabolism (Kruse et al, 2005;Esquível et al, 2006;Chochois et al, 2009); introduction of foreigner genes to enhance carbon utilization (Doebbe et al, 2007); role of electron transport chain and electron pools, the role of D1 protein (Faraloni and Torzillo, 2010); the effect of light-harvesting antenna size and the photosynthetic light utilization efficiency (Tetali et al, 2007); the role of Rubisco in the accumulation of electron pool (Hemschemeier et al, 2008); the molecular evolution of hydrogenase enzymes (Meyer, 2007;Kim and Kim, 2011;Oh et al, 2011); and the oxygen sensitivity of hydrogenase enzymes (Ghirardi et al, 2006;Nagy et al, 2007;Boyd et al, 2009;Meyer, 2007).…”

Photofermentive hydrogen production by Rhodobacter sphaeroides S10 using mixed organic carbon: Effects of the mixture composition

“…Although much effort is underway for understanding and engineering of metabolic pathways involved in the production of hydrogen [21][22][23][24][25][26], the need for suitably formulating a mixed substrate remains for attaining optimal production kinetics. In the presence of multiple sugars such as glucose and xylose, bacteria generally consume glucose preferentially and leave the other substrate in the medium for later use [27][28][29].…”

Modeling of a Cell-Free Synthetic System for Biohydrogen Production

“…Biological production is based on exploitation of micro-organisms with different biochemical pathways proficient at hydrogen production. Different pathways exist to produce hydrogen: dark fermentation from sugar, biophotolysis from absorption of light and water, and photo fermentation from absorption of light and organic acid [1][2][3]. Each pathway has a specific enzymes set but they suffer of a low yield hydrogen production.…”