We report a precise determination of the lifetime of the (4p) 2 P 3/2 state of 40 Ca + , τP 3/2 = 6.639(42) ns, using a combination of measurements of the induced light shift and scattering rate on a single trapped ion. Good agreement with the result of a recent high-level theoretical calculation, 6.69 (6) ns [Safronova et al., PRA 83, 012503 (2011)], but a 6-σ discrepancy with the most precise previous experimental value, 6.924(19) ns [Jin et al., PRL 70, 3213 (1993)] is found. To corroborate the consistency and accuracy of the new measurements, relativistically corrected ratios of reduceddipole-matrix elements are used to directly compare our result with a recent result for the P 1/2 state, yielding a good agreement. The application of the present method to precise determinations of radiative quantities of molecular systems is discussed.