CurvChip - chip platform for investigating cell responses to curved surface features

Kemkemer, Ralf; Frey, Kerstin; Fischer, Alena; Krastev, Rumen

doi:10.1515/cdbme-2018-0108

Cited by 2 publications

(3 citation statements)

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“…PDMS (10:1, curing agent:base, Sylgard®184 Silicone Elastomer Kit, Dow Corning) repetitive molding was fabricated as described in our previous work [3]. A Photoresist AZ ® 40XT (MircoChemicals GmbH, Ulm, Germany) was utilized to fabricate a chip with curvature structures.…”

Section: Materials and Methods 21 Materialsmentioning

confidence: 99%

“…A Photoresist AZ ® 40XT (MircoChemicals GmbH, Ulm, Germany) was utilized to fabricate a chip with curvature structures. This combined photolithography with a thermal reflow process [3]. PDMS microfluidic chip: For coating alginate on microfluidic chip surfaces, PDMS was poured into a 3D printed master with the negative chip structures, having channels of 500 × 20 µm (width × thickness) as well as reservoir and connection units.…”

Section: Materials and Methods 21 Materialsmentioning

confidence: 99%

“…Moreover, topography, especially smoothly curved features, is another relevant parameter in mimicking the native cellular environment. Topographies are omnipresent in situ and are such artificial surface features are important in cell research and development of improved implant surfaces [3]. To fabricate either microfluidic platforms or cell culture substrates with topography in lab environments, one of the most frequently used materials is poly(dimethylsiloxane) (PDMS).…”

Section: Introductionmentioning

confidence: 99%

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Surface modification of Polydimethylsiloxane by hydrogels for microfluidic applications

Kemkemer

Zhang

Linxiao

et al. 2019

Current Directions in Biomedical Engineering

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In vitro, hydrogel-based ECMs for functionalizing surfaces of various material have played an essential role in mimicking native tissue matrix. Polydimethylsiloxane (PDMS) is widely used to build microfluidic or organ-on-chip devices compatible with cells due to its easy handling in cast replication. Despite such advantages, the limitation of PDMS is its hydrophobic surface property. To improve wettability of PDMS-based devices, alginate, a naturally derived polysaccharide, was covalently bound to the PDMS surface. This alginate then crosslinked further hydrogel onto the PDMS surface in desired layer thickness. Hydrogel-modified PDMS was used for coating a topography chip system and in vitro investigation of cell growth on the surfaces. Moreover, such hydrophilic hydrogel-coated PDMS is utilized in a microfluidic device to prevent unspecific absorption of organic solutions. Hence, in both exemplary studies, PDMS surface properties were modified leading to improved devices.

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Section: Materials and Methods 21 Materialsmentioning

confidence: 99%

Section: Materials and Methods 21 Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Surface modification of Polydimethylsiloxane by hydrogels for microfluidic applications

Kemkemer

Zhang

Linxiao

et al. 2019

Current Directions in Biomedical Engineering

Self Cite

View full text Add to dashboard Cite

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Curvature Perception of Mesenchymal Cells on Mesoscale Topographies

Frey,

Brunner,

Curio

et al. 2024

Adv Healthcare Materials

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Cells can sense geometrical cues with sizes of several tens of micrometers in their vicinity. Recent in vitro studies show that cells can adapt their shape, align along specific directions, or regulate other cellular functions when grown on surfaces with curvatures larger than their size. Although possible mechanisms for such responses like the alignment along axial cues have been suggested, a detailed understanding of the involved cellular processes remains open. This work addresses this gap by systematically investigating mesenchymal cell and nucleus orientation responses using a low‐cost model surface platform, the CurvChip. Using an array of cylindrically curved topographies with radii of curvatures ranging from tens to hundreds of micrometers, the contact guidance response of cells and nuclei is quantified in dependence on substratum curvature and manipulation of cytoskeletal components. Results suggest a desired perceived curvature for the investigated cells, and a very sensitive and robust curvature perception mechanism, as the effect of pharmacological manipulation of cytoskeletal components is relatively small. Furthermore, a comparison with previously published work strengthens the hypothesis of an involvement of the nucleus in the cell response to three‐dimensional (3D) curvatures.

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CurvChip - chip platform for investigating cell responses to curved surface features

Cited by 2 publications

References 0 publications

Surface modification of Polydimethylsiloxane by hydrogels for microfluidic applications

Surface modification of Polydimethylsiloxane by hydrogels for microfluidic applications

Curvature Perception of Mesenchymal Cells on Mesoscale Topographies

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