Custom-tailored adsorbers: A molecular dynamics study on optimal design of ion exchange chromatography material

Lang, K.; Kittelmann, Jörg; Pilgram, Florian; Osberghaus, Anna; Hubbuch, Jürgen

doi:10.1016/j.chroma.2015.08.021

Cited by 3 publications

(1 citation statement)

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“…The stationary phase used in IE chromatography has charged ligands that get involved in an electrostatic interaction with biomolecules in the mobile phase. The strength of the interaction between protein molecules and the IE column is based on the Z number, which is an indication of the number of binding sites on the protein with respect to ligands [ 76 ]. The mobile phase is maintained at a low–medium ionic strength in the beginning of the separation run.…”

Section: Isolation Purification and Recovery Of Enzymesmentioning

confidence: 99%

Microbial Enzyme Production Using Lignocellulosic Food Industry Wastes as Feedstock: A Review

Ravindran¹,

Jaiswal²

2016

Bioengineering

121

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Enzymes are of great importance in the industry due to their substrate and product specificity, moderate reaction conditions, minimal by-product formation and high yield. They are important ingredients in several products and production processes. Up to 30% of the total production cost of enzymes is attributed to the raw materials costs. The food industry expels copious amounts of processing waste annually, which is mostly lignocellulosic in nature. Upon proper treatment, lignocellulose can replace conventional carbon sources in media preparations for industrial microbial processes, such as enzyme production. However, wild strains of microorganisms that produce industrially important enzymes show low yield and cannot thrive on artificial substrates. The application of recombinant DNA technology and metabolic engineering has enabled researchers to develop superior strains that can not only withstand harsh environmental conditions within a bioreactor but also ensure timely delivery of optimal results. This article gives an overview of the current complications encountered in enzyme production and how accumulating food processing waste can emerge as an environment-friendly and economically feasible solution for a choice of raw material. It also substantiates the latest techniques that have emerged in enzyme purification and recovery over the past four years.

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Section: Isolation Purification and Recovery Of Enzymesmentioning

confidence: 99%