Customized hydrogel substrates for serum-free expansion of functional hMSCs

Abstract

Synthetic hydrogel arrays combined with a design of experiments approach identified hydrogel compositions for media-agnostic human mesenchymal stromal cell culture.

Development and characterization of Factor Xa‐responsive materials for applications in cell culture and biologics delivery

“…To assess MSC differentiation capacity following FXa‐mediated cell harvest, cells were differentiated along their osteogenic and adipogenic lineages using previously established protocols 30 . MSCs were seeded onto 48‐well plates (Fisher Scientific) at 5000 cells/cm 2 , where they were cultured for 5 days until reaching confluency.…”

“…To assess MSC differentiation capacity following FXa-mediated cell harvest, cells were differentiated along their osteogenic and adipogenic lineages using previously established protocols. 30 MSCs were seeded onto 48-well plates (Fisher Scientific) at 5000 cells/cm 2 , where they were cultured for 5 days until reaching confluency. For osteogenic differentiation, cells were cultured in osteogenic medium consisting of 0.1 μM dexamethasone (Sigma-Aldrich), 10 mM β-glycerol phosphate (Sigma-Aldrich), 50 μM ascorbic acid 2-phosphate (Sigma-Aldrich) in αMEM (Gibco) with 10% FBS and 1Â penicillin/streptomycin (Gibco) for 21 days, with media exchanges after every 3 days.…”

Tissue engineering modalities in skeletal muscles: focus on angiogenesis and immunomodulation properties

“…Hence, stimulation of an angiogenic response is parallel to functional tissue regeneration and efficient regenerative outcomes [ 102 ]. One reason that causes muscular mass atresia and injury is the lack of a suitable supporting vasculature system [ 258 ]. The term angiogenesis is defined as the formation of de novo vessels from the preexisting network in response to several signaling molecules during physiological and pathological conditions [ 259 ].…”

Biomaterials for protein delivery for complex tissue healing responses

“…Several studies have demonstrated the utility of statistical optimization techniques, such as design of experiments, to optimize biomaterial properties achieve specific outcomes, such as cell response and gelation kinetics. [187][188][189] Additionally, we and others have employed computational bio-transport models to predict protein release kinetics from biomaterials and tune biomaterial properties to achieve desired release rates in vitro and in vivo. 10,64,77 Limasale et al demonstrated that a reaction-diffusion model could be used to predict the effect of heparin content and sulphation pattern on VEGF release, as well as the spatial distribution of VEGF throughout the hydrogel.…”