Customized platelet-rich plasma with transforming growth factor β1 neutralization antibody to reduce fibrosis in skeletal muscle

Li, Hongshuai; Hicks, Justin J.; Wang, Ling; Oyster, Nick; Philippon, Marc J.; Hurwitz, Shepard R.; Hogan, MaCalus V.; Huard, Johnny

doi:10.1016/j.biomaterials.2016.02.017

Cited by 95 publications

(86 citation statements)

References 59 publications

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“…For example, delivery of PRP post-neutralizing TGF-β1 enhances healing and reduces fibrosis upon skeletal muscle injury. 31 These studies indicate that preparations, which can sustain release and tailor PRP components can enhance therapy efficacy.…”

Section: Discussionmentioning

confidence: 97%

Platelet‐Rich Plasma Released From Polyethylene Glycol Hydrogels Exerts Beneficial Effects on Human Chondrocytes

Jain

Chinzei

Blanco

et al. 2019

Journal Orthopaedic Research

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Osteoarthritis (OA) is a debilitating joint disease resulting from chronic joint inflammation and erosion of articular cartilage. A promising biological treatment for OA is intra‐articular administration of platelet‐rich plasma (PRP). However, immediate bolus release of growth factors limits beneficial therapeutic effects of PRP, thus necessitating the demand for sustained release platforms. In this study, we evaluated the therapeutic value of PRP released from a polyethylene glycol (PEG) hydrogel on articular chondrocytes/cartilage explants derived from OA patients. Lyophilized PRP (PRGF) was encapsulated in PEG hydrogels at 10% w/v and hydrogel swelling, storage modulus and degradation and PRGF release kinetics were determined. PRGF releasate from the hydrogels was collected on day 1, 4, and 11. Encapsulation of PRGF at 10% w/v in PEG hydrogels had minimal effect on hydrogel properties. PRGF was released with an initial burst followed by sustained release until complete hydrogel degradation. Effect of PRGF releasates and bolus PRGF (1% w/v PRGF) on patient‐derived cartilage explants or chondrocytes was assessed by chondrocyte proliferation (pico‐green assay), gene expression for COL1A1, COL2A1, MMP13, COX2, and NFKB1 (real‐time polymerase chain reaction), and measurement of nitric oxide concentration (Griess’ assay). Compared to bolus PRGF, PRGF releasates enhanced chondrocyte proliferation, suppressed the expression of genes like MMP13, NFKB1, COL1A1, and COL2A1 and reduced levels of nitric oxide. Taken together, these results indicate that release of PRGF from PEG hydrogels may improve the therapeutic efficacy of PRP and merits further investigation in an animal model of OA. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 37:2401–2410, 2019

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Section: Discussionmentioning

confidence: 97%

Platelet‐Rich Plasma Released From Polyethylene Glycol Hydrogels Exerts Beneficial Effects on Human Chondrocytes

Jain

Chinzei

Blanco

et al. 2019

Journal Orthopaedic Research

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“…Science concerning PRP is improving, and PRP subtypes with increased concentration in leukocytes or in specific growth factors may be the key for further developments of this therapy. Since neovascularisation of the tendon seems to be associated with impairment and pain, control of vascular proliferation by subtracting or adding specific growth factors to PRP is at present a most important scientific challenge 92. At present, no modality or conservative intervention can guarantee the resolution of symptoms of IAT.…”

Section: Discussionmentioning

confidence: 99%

Achilles insertional tendinopathy: state of the art

Maffulli

Saxena²,

Wagner

et al. 2019

Journal of ISAKOS

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“…TA muscles were flash‐frozen in 2‐methylbutane precooled with liquid nitrogen and transverse cryo‐sections were obtained. Sections were stained with hematoxylin and eosin (H&E) and Masson modified trichrome stainings (IMEB, Chicago, IL) as described previously …”

Section: Methodsmentioning

confidence: 99%

“…Sections were stained with hematoxylin and eosin (H&E) and Masson modified trichrome stainings (IMEB, Chicago, IL) as described previously. 20 Micro-Computed Tomography. Lumbar spine and femur were collected at sacrifice and fixed in 10% neutral buffered formalin for 24 h. The samples were then scanned in 70% ethanol using Viva CT 40 (SCANCO Medical, Switzerland) with settings: energy 70 kv, intensity 114 μA, integration time 200 ms, and isotropic voxel size of 35 μm.…”

Section: Methodsmentioning

confidence: 99%

Altered bone‐regulating myokine expression in skeletal muscle Of Duchenne muscular dystrophy mouse models

et al. 2018

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Customized platelet-rich plasma with transforming growth factor β1 neutralization antibody to reduce fibrosis in skeletal muscle

Cited by 95 publications

References 59 publications

Platelet‐Rich Plasma Released From Polyethylene Glycol Hydrogels Exerts Beneficial Effects on Human Chondrocytes

Platelet‐Rich Plasma Released From Polyethylene Glycol Hydrogels Exerts Beneficial Effects on Human Chondrocytes

Achilles insertional tendinopathy: state of the art

Altered bone‐regulating myokine expression in skeletal muscle Of Duchenne muscular dystrophy mouse models

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