Customizing biohybrid cryogels to serve as ready-to-use delivery systems of signaling proteins

Sievers, Jana; Zimmermann, Ralf; Friedrichs, Jens; Pette, Dagmar; Limasale, Yanuar Dwi Putra; Werner, Carsten; Welzel, Petra B.

doi:10.1016/j.biomaterials.2021.121170

Cited by 9 publications

(12 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These cryogels are promising biomaterials for in vitro reconstruction of 3D bone marrow niches, as they are characterized by a highly interconnected macroporous structure and a high porosity (≈80%) comparable to that of trabecular bone. [62][63][64][65] They also have a high resistance to compressive loads. [65] After secreting ECM molecules onto the scaffold surface over 12 days, the MSCs were removed by decellularization (Figure 1a).…”

Section: Resultsmentioning

confidence: 99%

“…[62][63][64][65] They also have a high resistance to compressive loads. [65] After secreting ECM molecules onto the scaffold surface over 12 days, the MSCs were removed by decellularization (Figure 1a). With this strategy, we aimed to provide a customized 3D culture system that mimics the natural microenvironment of the human bone marrow niche and could ultimately be used for the ex vivo expansion of HSCs.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Combining Cryogel Architecture and Macromolecular Crowding‐Enhanced Extracellular Matrix Cues to Mimic the Bone Marrow Niche

Martínez‐Vidal

Magno

Welzel

et al. 2022

Macro Chemistry & Physics

Self Cite

View full text Add to dashboard Cite

Transplantation of hematopoietic stem cells (HSCs)—rare cells that can self‐renew and differentiate into cells of all hematopoietic lineages—is an important treatment option for patients with hematologic diseases. Key to the regenerative potential of HSCs is their natural in vivo microenvironment—the bone marrow niche—but in vitro engineering approaches to recapitulate the biochemical and biophysical properties of the bone marrow niche are still insufficiently explored. Herein, modular macroporous cryogels, mimicking the natural 3D architecture of trabecular bone, are seeded with human bone marrow‐derived mesenchymal stromal cells and decellularized to generate tissue‐specific extracellular matrix (ECM)‐decorated scaffolds. To recapitulate the physiological concentration of biomolecules in bone marrow, the cell culture medium is supplemented with macromolecular crowders that modulate the composition, ultrastructure, and mechanical properties of the cell‐deposited ECM. The ECM‐coated cryogel scaffolds generated under crowding conditions exhibit physicochemical properties similar to those of the endosteal niche of the bone marrow. The ECM‐functionalized cryogels presented could be used in the future for the ex vivo expansion of HSCs for transplantation and may also aid in the development of more realistic hematological disease models.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Combining Cryogel Architecture and Macromolecular Crowding‐Enhanced Extracellular Matrix Cues to Mimic the Bone Marrow Niche

Martínez‐Vidal

Magno

Welzel

et al. 2022

Macro Chemistry & Physics

Self Cite

View full text Add to dashboard Cite

show abstract

“…Arginine–glycine–aspartate peptide (RGD) is a peptide derived from fibronectin or collagen, which provides cells with integrin sites and links to integrins that activate cells, thereby allowing cells to adhere to the ECM [ 213 ]. Modification of macroporous hydrogels with RGD is widely used, which successfully upregulates MSCs, GBM (which overexpresses the associated αvβ3 and αvβ5 binding integrins) [ 214 ], chondrocytes [ 193 ], PC12 cells [ 215 ], HEK 293 cells [ 208 ], and adipose-derived stem cells (ADSCs) [ 216 ]. Notably, a small amount of RGD was sufficient to support the adhesion of HUVECs to dextran macroporous hydrogels [ 217 ], suggesting that the presence or absence of adhesion molecules affects cell adhesion more than the concentration.…”

Section: Biophysical and Biochemical Factors Of Macroporous Hydrogels...mentioning

confidence: 99%

Recent Advances in Macroporous Hydrogels for Cell Behavior and Tissue Engineering

Wang

et al. 2022

Gels

View full text Add to dashboard Cite

Hydrogels have been extensively used as scaffolds in tissue engineering for cell adhesion, proliferation, migration, and differentiation because of their high-water content and biocompatibility similarity to the extracellular matrix. However, submicron or nanosized pore networks within hydrogels severely limit cell survival and tissue regeneration. In recent years, the application of macroporous hydrogels in tissue engineering has received considerable attention. The macroporous structure not only facilitates nutrient transportation and metabolite discharge but also provides more space for cell behavior and tissue formation. Several strategies for creating and functionalizing macroporous hydrogels have been reported. This review began with an overview of the advantages and challenges of macroporous hydrogels in the regulation of cellular behavior. In addition, advanced methods for the preparation of macroporous hydrogels to modulate cellular behavior were discussed. Finally, future research in related fields was discussed.

show abstract

“…Biomimetic cryogel design, resembling natural tissue architectures, may enhance tissue engineering and regenerative medicine [ 22 ]. Additionally, long-term stability, biodegradability, combination therapies, and drug delivery systems are areas that require further research [ 22 , 23 , 24 ]. In vivo evaluation and clinical trials are crucial to ascertain the safety and efficacy of cryogels [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Cryogels: Advancing Biomaterials for Transformative Biomedical Applications

2023

View full text Add to dashboard Cite

Cryogels, composed of synthetic and natural materials, have emerged as versatile biomaterials with applications in tissue engineering, controlled drug delivery, regenerative medicine, and therapeutics. However, optimizing cryogel properties, such as mechanical strength and release profiles, remains challenging. To advance the field, researchers are exploring advanced manufacturing techniques, biomimetic design, and addressing long-term stability. Combination therapies and drug delivery systems using cryogels show promise. In vivo evaluation and clinical trials are crucial for safety and efficacy. Overcoming practical challenges, including scalability, structural integrity, mass transfer constraints, biocompatibility, seamless integration, and cost-effectiveness, is essential. By addressing these challenges, cryogels can transform biomedical applications with innovative biomaterials.

show abstract

Customizing biohybrid cryogels to serve as ready-to-use delivery systems of signaling proteins

Cited by 9 publications

References 75 publications

Combining Cryogel Architecture and Macromolecular Crowding‐Enhanced Extracellular Matrix Cues to Mimic the Bone Marrow Niche

Combining Cryogel Architecture and Macromolecular Crowding‐Enhanced Extracellular Matrix Cues to Mimic the Bone Marrow Niche

Recent Advances in Macroporous Hydrogels for Cell Behavior and Tissue Engineering

Cryogels: Advancing Biomaterials for Transformative Biomedical Applications

Contact Info

Product

Resources

About